Interacting Effects of Cell Size and Temperature on Gene Expression, Growth, Development and Swimming Performance in Larval Zebrafish

Pol, Iris L. E. van de; Hermaniuk, Adam; Verberk, W.C.E.P.

doi:10.3389/fphys.2021.738804

Cited by 6 publications

(6 citation statements)

References 63 publications

(85 reference statements)

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“…When an activity of the Na + /K + ATPase was inhibited, the metabolic difference between diploids and triploids was abolished, suggesting that less energy is required to maintain plasma membrane potential in triploids due to the lower cell surface area to volume ratio. Also, the similar low metabolic phenotypes of polyploid cells were consistent with the data in yeast [88] and zebrafish [93]. It would be important to confirm whether the metabolic rate alteration contributes to the higher stress resistance of polyploid cells especially to radiation tolerance.…”

Section: The Protein Synthesis and Metabolism In Polyploid Cellssupporting

confidence: 80%

Polyploid Cancer Cell Models in Drosophila

Wang,

Tamori

2024

Genes

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Cells with an abnormal number of chromosomes have been found in more than 90% of solid tumors, and among these, polyploidy accounts for about 40%. Polyploidized cells most often have duplicate centrosomes as well as genomes, and thus their mitosis tends to promote merotelic spindle attachments and chromosomal instability, which produces a variety of aneuploid daughter cells. Polyploid cells have been found highly resistant to various stress and anticancer therapies, such as radiation and mitogenic inhibitors. In other words, common cancer therapies kill proliferative diploid cells, which make up the majority of cancer tissues, while polyploid cells, which lurk in smaller numbers, may survive. The surviving polyploid cells, prompted by acute environmental changes, begin to mitose with chromosomal instability, leading to an explosion of genetic heterogeneity and a concomitant cell competition and adaptive evolution. The result is a recurrence of the cancer during which the tenacious cells that survived treatment express malignant traits. Although the presence of polyploid cells in cancer tissues has been observed for more than 150 years, the function and exact role of these cells in cancer progression has remained elusive. For this reason, there is currently no effective therapeutic treatment directed against polyploid cells. This is due in part to the lack of suitable experimental models, but recently several models have become available to study polyploid cells in vivo. We propose that the experimental models in Drosophila, for which genetic techniques are highly developed, could be very useful in deciphering mechanisms of polyploidy and its role in cancer progression.

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Section: The Protein Synthesis and Metabolism In Polyploid Cellssupporting

confidence: 80%

Polyploid Cancer Cell Models in Drosophila

Wang,

Tamori

2024

Genes

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“…However, it remains unclear whether the observed CS activity following stress exposure is attributable to ploidy, as studies of CS activity within other taxa have produced mixed results. For example, triploid zebrafish displayed the same CS enzyme activity following temperature stress (van de Pol et al, 2021), while triploid white sturgeon displayed a marked decrease with respect to diploids (Leal et al, 2019). Nevertheless, meaningful differences in standard metabolic rate were observed across ploidy within this study, with triploids decreasing and diploids increasing oxygen consumption following multiple stress exposure (Figure 3F).…”

Section: Discussionmentioning

confidence: 99%

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

George

Cattau

Middleton

et al. 2023

Preprint

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Polyploidy has been shown to negatively impact environmental stress tolerance, resulting in increased susceptibility to extreme climate events such as marine heatwaves (MHWs). In this study, we used the response of the Pacific oysterCrassostrea gigasto MHWs as a model system to identify key ploidy-specific differences in the physiological and transcriptomic response of oysters to environmental stress. In this study, adult diploid (2n) and triploid (3n) oysters were exposed to elevated seawater temperature (single stressor; 30°C), elevated temperature followed by acute desiccation stress (multiple stressor; 30°C + emersion at an aerial temperature of 44°C for 4h), or a control (17°C) within a hatchery setting. Oyster mortality rate was elevated within stress treatments with respect to the control and was significantly higher in triploids than diploids following multiple stress exposure (36.4% vs. 14.8%). Triploids within the multiple stressor treatment exhibited signs of energetic limitation, including metabolic depression, a significant reduction in ctenidium Na+/K+ATPase activity, and the dysregulated expression of key genes associated with heat tolerance, the inhibition of apoptosis, and mitochondrial function. Functional enrichment analysis of ploidy-specific gene sets identified that biological processes associated with metabolism, stress tolerance, and immune function were overrepresented within triploids across stress treatments. Our results demonstrate that triploidy impacts the transcriptional regulation of key metabolic processes that underly the environmental stress response of Pacific oysters, resulting in downstream shifts in physiological tolerance limits that may be detrimental to survival. The impact of chromosome set manipulation on the climate resilience of marine populations has important implications for the adaptability of marine populations and domestic food security within future climate scenarios, especially as triploidy induction becomes an increasingly popular tool to elicit reproductive control across a wide range of marine organisms used within marine aquaculture.

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“…In addition, the cells were significantly reduced in size when the temperature was increased by both 5 • C and 8 • C. Cell size is an important feature for understanding the thermal biology of ectotherms, as the regulation and consequences of cell size appear to be temperature-dependent [30][31][32][33]. Thus, the surface-to-volume ratio is greater in smaller cells than in larger cells of the same shape.…”

Section: Discussionmentioning

confidence: 99%

Effects of Temperature Adaptation on the Metabolism and Physiological Properties of Sturgeon Fish Larvae Cell Line

Lutze,

Brenmoehl,

Tesenvitz

et al. 2024

Cells

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This study investigated how Atlantic sturgeon cells respond to elevated temperatures, shedding light on the potential impacts of climate change on fish. Atlantic sturgeon (Acipenser oxyrinchus), an IUCN (International Union for Conservation of Nature) Red List species and evolutionarily related to paleonisiform species, may have considerable physiological adaptability, suggesting that this species may be able to cope with changing climatic conditions and higher temperatures. To test this hypothesis, the AOXlar7y cell line was examined at 20 °C (control) and at elevated temperatures of 25 °C and 28 °C. Parameters including proliferation, vitality, morphology, and gene expressions related to proliferation, stemness, and stress were evaluated. Additionally, to achieve a comprehensive understanding of cellular changes, mitochondrial and metabolic activities were assessed using Seahorse XF96. AOXlar7y cells adapted to 28 °C exhibited enhanced mitochondrial adaptability, plasticity, heightened cell proliferation, and increased hsp70 expression. Increased baseline respiration indicated elevated ATP demand, which is potentially linked to higher cell proliferation and heat stress defense. Cells at 28 °C also displayed elevated reserve respiration capacity, suggesting adaptation to energy demands. At 25 °C, AOXlar7y cells showed no changes in basal respiration or mitochondrial capacity, suggesting unchanged ATP demand compared to cells cultivated at 20 °C. Proliferation and glycolytic response to energy requirements were diminished, implying a connection between glycolysis inhibition and proliferation suppression. These research results indicate sturgeon cells are capable of withstanding and adapting to an 8 °C temperature increase. This cellular analysis lays a foundation for future studies aimed at a deeper understanding of fish cell physiological adaptations, which will contribute to a better knowledge of environmental threats facing Atlantic sturgeon and fish populations amid climate change.

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Interacting Effects of Cell Size and Temperature on Gene Expression, Growth, Development and Swimming Performance in Larval Zebrafish

Cited by 6 publications

References 63 publications

Polyploid Cancer Cell Models in Drosophila

Polyploid Cancer Cell Models in Drosophila

Triploid Pacific oysters exhibit stress response dysregulation and elevated mortality following marine heatwaves

Effects of Temperature Adaptation on the Metabolism and Physiological Properties of Sturgeon Fish Larvae Cell Line

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