Interações entre fungos micorrízicos arbusculares e a microbiota de solos

Ferreira, Dorotéia Alves

doi:10.11606/t.11.2016.tde-27092016-163610

Cited by 1 publication

(1 citation statement)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, different mycorrhizae related to P solubilization were aplied in coinoculation with 33.1: Rhizophagus intraradices, from commercial product Rootella® BR, Rhizophagus clarus and Dentiscutata heterogama from Soil Microbiology Laboratory, (ESALQ/USP). R. clarus and D. heterogama have been previously identified as colonizers of sugarcane roots (Ferreira, 2016).…”

Section: Microbial Strainsmentioning

confidence: 99%

Unraveling the molecular mechanisms involved in plant growth promotion and soil bacterial community modulation by >i<Pantoea agglomerans>/i< 33.1

Ferreira

View full text Add to dashboard Cite

Protein Secretion Systems (PSS) are essential molecular machineries to bacterial survival and biotic and abiotic interactions. The PSS have been reported as an important system in beneficial plant/bacteria interactions, delivering beneficial substrates to the host. Pantoea agglomerans 33.1 is a phosphate solubilizing bacterium, with the capacity to promote plant growth described for several crops. Considering the agricultural potential of 33.1, a comprehensive understanding of its mechanisms to promote plant growth is vital. Thus, this research aimed to investigate the role of PSS in plant growth promotion by 33.1 through gene knockout via CRISPR-Cas9. To achieve this knowledge, the whole-genome of 33.1 was sequenced and assembled, genes related to the main growth-promoting pathways were annotated, and those related to PSS were mining and manually annotated, and compared to those found in other P. agglomerans strains. Based on this annotation, two genes of different PSS were selected for knockout through CRISPR-Cas9, employing the two-plasmid strategy. We obtained a total genome of 4,844,324 base-pairs (bp) divided into the chromosome and four plasmids, that harbor genes of processes related to plant growth promotion, such as P metabolism and indole-3-acetic-acid (IAA) production. The classical secretion systems 1, 5a, 5b, and 6, the secretion pathways Sec and Tat, and the appendages and accessories Bam Complex and T4P were found in 33.1. Our PSS prediction is in line with what was observed in the comparative genomics results, where systems 1 and 5 were shown to be the most conserved among the different strains of P. agglomerans. Concerning the gene editing, we obtained the 33.1.:pCas strain and the bepC and prn genes were properly cloned into the pTarget series. Despite the two-plasmid system required for CRISPR-Cas9 have been correctly assembled, the system failed to knock out the Outer Membrane Protein (OMP) and the Autotransporter (AT) of the 33.1, from T1SS and T5aSS, respectively. Regardless of the unsuccessful efforts, our research provides important insights about the 33.1 biology and topics to be improved for knockout assays that will be continued by our group, until the mutants are obtained.

show abstract

Section: Microbial Strainsmentioning

confidence: 99%

Unraveling the molecular mechanisms involved in plant growth promotion and soil bacterial community modulation by >i<Pantoea agglomerans>/i< 33.1

Ferreira

View full text Add to dashboard Cite

show abstract

Interações entre fungos micorrízicos arbusculares e a microbiota de solos

Cited by 1 publication

References 57 publications

Unraveling the molecular mechanisms involved in plant growth promotion and soil bacterial community modulation by >i<Pantoea agglomerans>/i< 33.1

Unraveling the molecular mechanisms involved in plant growth promotion and soil bacterial community modulation by >i<Pantoea agglomerans>/i< 33.1

Contact Info

Product

Resources

About