Inter-test reliability of the anti-RESA indices based on ELISA tests using eluates from whole blood spots dried on filter paper

Abstract

The ring-infected erythrocyte surface antigen (RESA), is one of the falciparum malaria vaccine candidates rarely studied in Brazil. Fieldwork logistics to conduct serology studies is simplified when eluates from whole blood dried on filter paper can be used. Therefore, this study aimed to assess the inter-test reliability for the anti-RESA ELISA-based indices using eluates from filter paper and from serum samples. The study population consisted of 210 individuals (Brazil) from whom matched samples were collected. Anti-RESA ELISA-based index means (+/- S.D.) were 15.29% (+/-28.13%) for filter paper and 11.79% (+/-23.67%) for serum samples. The intra-class correlation coefficient was estimated to be 82.38%, indicating high test reliability. However, there was a significant tendency for filter paper test results to have higher values than serum sample test results (P < 0.001). Explanations for this finding may be the presence of haemoglobin in the eluates from filter paper, which may interfere with ELISA testing.

Self‐reported compliance with last malaria treatment and occurrence of malaria during follow‐up in a Brazilian Amazon population

“…§ Anti-RESA ELISA-based index ¼ [(sample OD-negative control OD)/(positive control OD-negative control OD)] · 100; OD ¼ optical density; Anti-RESA ELISA-based indices derived from filter paper tests were corrected based on their linear relationship to the serumbased results (corrected index ¼ 0.7236 · observed index) (Duarte et al 2002).…”

“…-Anti-RESA ELISA-based index ¼ [(sample OD-negative control OD)/(positive control OD-negative control OD)] · 100; OD ¼ optical density; Anti-RESA ELISA-based indices derived from filter paper tests were corrected based on their linear relationship to the serum-based results (corrected index ¼ 0.7236 · observed index) (Duarte et al 2002). ** Other interaction terms tested were between compliance with last malaria treatment and: malaria episodes during lifetime (P ¼ 0.079), months lived in mining environment (P ¼ 0.650), anti-RESA ELISA-based index (P ¼ 0.517), spleen size (P ¼ 0.851) and months since last malaria episode (P ¼ 0.581).…”

Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review

“…Overall, DBS and serum samples from 16,255 individuals were included: 13,742 (84.5%) adults, 560 (3.4%) 5-to 17-year old's and 2,082 (12.8%) less than five years old. Two studies reported antibodies against hepatitis A [14,15], nine hepatitis B [16][17][18][26][27][28]46,48,51], ten hepatitis C [19][20][21][22][26][27][28]37,46,48], eight HIV [23][24][25][26][27][28]46,50], three human papillomavirus (HPV) [29,30,37], three measles [31,32,42], three rubella [33,34,42], two syphilis [40,46], two H. pylori [35,47] and two malaria [38,49]. Twelve papers reported on Chagas disease [35], Epstein-Barr virus [36], HPV, H. pylori, hepatitis C and polyomavirus [37], Strong...…”

“…Twelve papers reported on Chagas disease [35], Epstein-Barr virus [36], HPV, H. pylori, hepatitis C and polyomavirus [37], Strongyloidiasis [39], pertussis [41], hepatitis E [56], Vibrio cholera [2], measles, mumps and rubella [42], tuberculosis and cytomegalovirus [43], toxoplasmosis [44], trypanosoma [45], Covid-19 [52], respectively. Twelve studies were conducted in Europe [14,18,21,27,29,30,35,[41][42][43]50,52], four in North America [19,20,32,36], eleven in South America [15][16][17]22,24,34,38,39,48,49,51], seven in Africa [23,25,26,31,40,44,45] and five in Asia [28,33,…”

Dried blood spots as a source of anti-malarial antibodies for epidemiological studies

“…More recently blood spots have been used as a source of DNA for screening for genetic abnormalities in newborns, for example for cystic fibrosis and haemoglobinopathies [ 2 , 3 ]. Blood spots have been used for monitoring antibodies against several viral [ 4 - 8 ], bacterial [ 9 , 10 ] and other [ 11 - 16 ] pathogens, storage of monoclonal antibodies[ 17 ] and, increasingly, for screening for HIV infection [ 18 - 20 ], both as a source of antibodies [ 9 , 21 , 22 ] and for virus detection by PCR. Dried blood spots have been particularly useful for isolating parasite DNA in mapping the spread of drug resistance in malaria parasites [ 23 , 24 ]…”