Inter-species hemorheologic differences in arterial and venous blood

Abstract

Background: Hemorheologic factors such as red blood cell (RBC) aggregation and deformability differ according to species. In many comparative hemorheologic studies, only venous blood samples have been used for measurements. There is little published information comparing arterial and venous blood differences between species. We compared hemorheologic factors in arterial and venous blood in rats, dogs and humans.Methods: Nineteen dogs and 12 rats were used. Human blood was obtained from 12 healthy volunteers. Blood gas analysis, hematocrit and elongation index which represents RBC deformability were measured in arterial and venous blood samples. The critical shear stress and aggregation index, both of which represent RBC aggregation, were also measured in arterial and venous blood.Results: There were no arterial or venous differences in hematocrit, critical shear stress, or elongation index in dogs. In rats, RBC aggregation was not measurable. The hematocrit and elongation index of arterial blood were significantly lower than those of venous blood in rats. There were no arterial or venous differences in hematocrit, aggregation index, or elongation index in humans.Conclusion: Arterial and venous hemorheologic factors differed depending on the species. Further standardization is necessary for the measurement of hemorheologic variables.

Sampling Site Matters When Counting Lymphocyte Subpopulations

“…Importantly, we calculated cell counts both with and without normalization accounting for the observed hemoconcentration in the venous circulation compared to the arterial circulation. Our observation of an increase in red blood cell count and hematocrit is supported by some [4]–[6] but not all published studies [1], [3], [7].…”

“…However, both in clinical practice and scientific studies on hematological and immunological diseases, physicians and researchers routinely rely on venipuncture as the common source of blood collection. Until now, only a few studies (Table S1) have looked at the differences in cell counts between the venous and arterial circulations, but no study has done this for lymphocyte subpopulations [1]–[7]. In this exploratory study we made within-individual analyses in 18 individuals comparing cell counts for different blood cell populations collected via the radial artery, a dorsal hand vein and an antecubital vein.…”

Comparison of Enterprise Point‐of‐Care and Nova Biomedical Critical Care Xpress analyzers for determination of arterialpH, blood gas, and electrolyte values in canine and equine blood

“…17 Species-specific differences in the HCT and the rheologic properties of red blood cells have been reported and could potentially lead to differences in these measurements. 20,21 In our study, HCT CV% did meet the quality goals for HCT based on canine HCT values. Because the HCT CV% was the same for both units, we concluded that precision was good for HCT for both analyzers.…”

Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging

“…Here we mostly used canine platelets to test the performance of the ITS because dog blood is more accessible than human blood while dog platelets bear similar biological traits to human platelets (LeVine et al 2014). Dogs have sizes close to humans, resulting in similar rheology and metabolic rates in blood (Son et al 2010). Dogs and humans have similar platelet counts, signaling pathways, and platelet sizes (Boudreaux et al 2007).…”