Inter-Relationship between Platelet-Derived Microparticles and Oxidative Stress in Patients with Venous Thromboembolism

Signorelli, Salvatore Santo; Conti, Gea Oliveri; Fiore, Maria; Elfio, Maria Grazia; Cristaldi, Antonio; Nicolosi, Ilenia; Zuccarello, Pietro; Zanoli, Luca; Gaudio, Agostino; Raimondo, Domenico Di

doi:10.3390/antiox9121217

Cited by 6 publications

(6 citation statements)

References 34 publications

(40 reference statements)

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“…The term thrombophilia is increasingly used to describe deep vein thrombosis (DVT) in individuals with genetic or acquired disorders [91] . Compared with controls, PMPs and phospholipid generation were higher and the prothrombinase-induced clotting time assay velocity was lower in patients with DVT [92] . Women with polycystic ovary syndrome (PCOS) are often obese and thought to have increased cardiovascular risk.…”

Section: Applicationsmentioning

confidence: 72%

Platelet-derived microparticles and their cargos: The past, present and future

Guo,

Cui,

Zheng

et al. 2024

Asian Journal of Pharmaceutical Sciences

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Section: Applicationsmentioning

confidence: 72%

Platelet-derived microparticles and their cargos: The past, present and future

Guo,

Cui,

Zheng

et al. 2024

Asian Journal of Pharmaceutical Sciences

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“…In the research, to reveal the mechanism of Y15, we investigated the effect of Y15 on FAK and Akt phosphorylation. The imbalance between oxidation and anti-oxidation is one of the important factors in the occurrence and development of oxidative damage [ 44 , 45 ]. Oxidative stress markers ROS and NO are essential regulators of endothelial function [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Focal Adhesion Kinase Inhibitor Inhibits the Oxidative Damage Induced by Central Venous Catheter via Abolishing Focal Adhesion Kinase-Protein Kinase B Pathway Activation

Wang

Lin

Jiang

et al. 2021

BioMed Research International

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The vascular injury induced by central venous catheter (CVC) indwelling is the basis for the occurrence and development of CVC-related complications, such as phlebitis, venous thrombosis, and catheter-related infections. Focal adhesion kinase (FAK) and FAK-protein kinase B (AKT) signaling pathway are of great significance in tissue repair after trauma. Here, we investigated the role and mechanism of the FAK inhibitor (1,2,4,5-phenyltetramine tetrahydrochloride (Y15)) in oxidative damage caused by CVC. EA.hy926 cells were divided into the control group (normal control), CVCs+scratches group (the intercepted CVC segments coculturing with scratched EA.hy926 cells), and CVCs+scratches+Y15 group (Y15 was added to the cell culture supernatant with CVCs + scratches at a final concentration of 50 μmol·L-1). New Zealand rabbits were randomly divided into the control group (normal control), CVC group (CVC was inserted through the rabbit’s right jugular vein to the junction of the right atrium and superior vena cava), and CVC+Y15 group (CVC was immersed in a 50 μmol·L-1 Y15 solutions before insertion). The levels of markers and proteins related to oxidative damage in cells, cell culture supernatant, serum, and external jugular vein were measured by commercial kits and western blot, respectively. We found that Y15 treatment significantly decreased ROS and MDA levels and increased cell viability, NO, and SOD levels in a time-dependent manner in rabbit serum and cell culture supernatant. In addition, Y15 effectively reduced the CVC-induced pathological changes of damaged vascular tissues. Y15 also downregulated the levels of p-FAK Tyr 397 and p-Akt Ser 473 in damaged external jugular vein and EA.hy926 cells. These findings suggest that Y15 alleviated CVC-induced oxidative damage to blood vessels by suppressing focal FAK-Akt pathway activation.

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“…The methodology for determining both MDA and 4-HNE has already been described [ 6 , 15 , 16 , 17 , 18 ].…”

Section: Study Methodologmentioning

confidence: 99%

“…MDA and 4-HNE were determined as complexes with thiobarbituric acid (TBA) [ 15 , 17 , 18 ]. Plasma samples were treated as described above, with the exception of the incubation temperature which was 90.0 °C.…”

Section: Study Methodologmentioning

confidence: 99%

Oxidative Status, Iron Plasma Levels in Venous Thrombosis Patients

Signorelli,

Barbagallo,

Oliveri Conti

et al. 2024

Antioxidants

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Exaggerated clot induces venous thrombosis (VTE); oxidative stress (OxS) can to be postulated as additional risk factor. This study evaluates firstly OxS by measuring surrogate biomarkers (malondialdehyde-MDA, 4-hydroxinonenal-4-HNE, superoxide desmutase enzyme (SOD)), secondly the iron (Fe) plasma level and thirdly the hepcidin protein (Hep) level in patients with VTE. A case control study was performed enrolling twenty hospitalized patients and an equal number of healthy individuals. In VTE patients, the following results were found. The MDA was 8.38 ± 0.5 µM/L, the 4-HNE measured 2.75 ± 0.03 µM/L and the SOD was 0.025 ± 0.01 U/mL. The I was 73.10 ± 10 µg/dL and the He was 4.77 ± 0.52 ng/mL. In the control group, the MDA measured 5.5 ± 0.6 µM/L, the 4-HNE 2.24 ± 0.021 µM/L and the SOD 0.08 ± 0.12 U/mL. The Hep was 2.1 ± 0.55 ng/mL and the Fe was 88.2 ± 9.19 µg/dL. Differences were statistically significant. Results suggest that in VTE there is activated OxS, Fe deregulation and over-production of Hep. Fe deregulation induces OxS, leading both to inflammation in the clot activator and stimulation of the pro-thrombotic status. The study highlights OxS and Fe and their regulation as intriguing indicators for risk of VTE.

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Inter-Relationship between Platelet-Derived Microparticles and Oxidative Stress in Patients with Venous Thromboembolism

Cited by 6 publications

References 34 publications

Platelet-derived microparticles and their cargos: The past, present and future

Platelet-derived microparticles and their cargos: The past, present and future

Focal Adhesion Kinase Inhibitor Inhibits the Oxidative Damage Induced by Central Venous Catheter via Abolishing Focal Adhesion Kinase-Protein Kinase B Pathway Activation

Oxidative Status, Iron Plasma Levels in Venous Thrombosis Patients

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