Inter-plot competition in hybrid maize multi-environment yield trials in Ethiopia can reduce rate of genetic gain

Abstract

Conducting large-scale multi-environment trials (METs) is practically challenging to breeding programs in Sub-Saharan Africa due to limitations of land, seed, and associated costs of phenotyping. As a result, early-generation hybrid maize trials are usually planted with single-row plots. In trials with single-row plots, genotypes compete with their neighbors for resources, and this biases the prediction of the genotype value. We demonstrate the impact of inter-plot competition in single-row multi-environment hybrid maize trials in Ethiopia through a motivating example assessing subsets of 478 maize hybrids in six trials in the 2019 and 2020 main season. The trials were planted in partially replicated design with single-row plots. Field spatial variability and inter-plot competition were jointly modelled in a linear mixed model framework to partition the total genetic effect into direct and neighbour effects. Both direct and neighbour genetic effects differed across the trials confirming the presence of genotype by environment interaction (GEI) for these genetic components. There was a rank change in genotype performance between the selections from the competition model in comparison to the standard MET model. The proportion of mismatch in the top 20% of the selected genotypes from the competition model and the standard MET model ranged from 20 – 70 % across the optimum nitrogen sites and up to 90 % in the low nitrogen site. The results demonstrated that inter-plot competition biases grain yield predictions in single-row plot hybrid maize METs and thus reduces the rate of genetic gain, if not accounted for using appropriate statistical models.