Inter-laboratory validation study of the skin2 dermal model ZK1100 and MTT cytotoxicity assay kits.

Taniguchi, Yuzo; Suzuki, Keiji; Nakajima, Keiya; Nakajima, Madoka; Miwa, Yoshihisa; Yumura, Yasushi; Satoh, Matoshi; Takeyoshi, Masahiro; Akie, Yasuki; Moriyasu, Matsuko; Yamamoto, Hiroshi; Katagiri, M.; Kazama, Akemi; Watanabe, Masami

doi:10.2131/jts.19.37

Cited by 7 publications

(3 citation statements)

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“…Basal keratinocytes formed a characteristic columnar layer with several suprabasal squamous cell layers covered by a thin stratum corneum. The extent of epidermal differentiation and functional properties of KFCs are detailed elsewhere (13,50,51,56,63).…”

Section: Methodsmentioning

confidence: 99%

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

et al. 1998

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Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. Predominantly a cutaneous pathogen, H. ducreyi is present in chancroid ulcers that are characterized by extensive neutrophil accumulation in intraepidermal lesions accompanied by a mononuclear infiltrate in the dermis. We used an in vitro human skin model composed of foreskin fibroblasts and keratinocytes to examine host skin cell interactions with H. ducreyi 35000. Bacteria replicated and persisted in artificial skin for at least 14 days. We observed H. ducreyi inside suprabasal keratinocytes using transmission electron microscopy. Although no bacteria were seen in the basal keratinocyte region, these cells were disrupted in infected cocultures.H. ducreyi infection stimulated increased secretion of interleukin-6 (IL-6) and IL-8 by skin cells. Conversely, tumor necrosis factor alpha and IL-1α levels were not elevated. IL-8 produced in response to H. ducreyi infection may be involved in recruiting polymorphonuclear leukocytes and other inflammatory cells, thereby contributing to the tissue necrosis and ulcer formation characteristic of chancroid.

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Section: Methodsmentioning

confidence: 99%

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

et al. 1998

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“…In Japan, the Japanese Society of Alternatives to Animals Experiments (JSAAE) is responsible for carrying out validation assays. In recent years, different studies have been developed to validate alternatives to the eye19–23 and skin24, 25 irritation in vivo test. However, despite the quantity and quality of the work carried out, few alternative methods to replace the classical Draize test have been accepted by the regulatory authorities.…”

Section: Validation Of Alternative Methodsmentioning

confidence: 99%

Alternative Methods for Eye and Skin Irritation Tests: An Overview

Vinardell

Mitjans

2008

Journal of Pharmaceutical Sciences

162

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“…Cells were incubated for 60 minutes. Culture medium with 1% DMSO (acting as an irritant [10] was used as a positive control for cell viability after irritation. All results are expressed as relative viability compared to cells grown in.…”

Section: Mtt Cell Viability Assaymentioning

confidence: 99%

Cytotoxic Effect on Corneal Surface of Multipurpose Soft Contact Lens Solution Which Contains Aloe Vera

Pintor

Martn-Gil²,

Carracedo³

et al. 2014

Biochem & Pharmacol

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Inter-laboratory validation study of the skin2 dermal model ZK1100 and MTT cytotoxicity assay kits.

Cited by 7 publications

References 0 publications

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

Haemophilus ducreyiInfection Causes Basal Keratinocyte Cytotoxicity and Elicits a Unique Cytokine Induction Pattern in an In Vitro Human Skin Model

Alternative Methods for Eye and Skin Irritation Tests: An Overview

Cytotoxic Effect on Corneal Surface of Multipurpose Soft Contact Lens Solution Which Contains Aloe Vera

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