Inter-Isolate Heterokaryosis in Pyricularia oryzae

Fatemi, Javad

doi:10.1094/phyto-68-1791

Cited by 14 publications

(13 citation statements)

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“…Many researchers have reported heterokaryosis and a parasexual cycle in this fungus 9,11,14,42,45,47 . Auxotrophic mutants have been used to study vegetative compatibility, heterokaryosis and parasexuality in Pyricularia oryzae 6,9,11 .…”

Section: Introductionmentioning

confidence: 77%

“…Auxotrophic mutants have been used to study vegetative compatibility, heterokaryosis and parasexuality in Pyricularia oryzae 6,9,11 . While many researchers have postulated that changes in pathogenicity occur through parasexual recom-JARQ 41 (2) 2007 M. T. Noguchi, N. Yasuda & Y. Fujita bination, only a few reports have dealt with the fitness of parasexual recombinants 31,32 , and experimental field data on parasexual recombinants are lacking.…”

Section: Introductionmentioning

confidence: 99%

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Fitness Characters in Parasexual Recombinants of the Rice Blast Fungus, Pyricularia oryzae

Noguchi

Yasuda

Fujita

2007

JARQ

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We examined the fitness characters in parasexual recombinants derived from cocultures of two isolates, NAO-02 (race 133.1) and TH77-1 (race 047.0), of the rice blast fungus, Pyricularia oryzae, in liquid yeast extract medium. The cocultured isolates were transferred to oatmeal agar to produce a conidial inoculum. Then, the conidial suspension was sprayed on the japonica rice cultivar Akiyutaka, which has two resistance genes to rice blast: Pik and Piz. Three pathogenic variants were isolated from the typical leaf blast lesions that were subsequently produced. We used randomly amplified polymorphic DNA (RAPD) to examine whether the variants were derived from parasexual recombination between the parents. Eleven RAPDs indicated that the variants possessed genomic DNA from both parents. After inoculating Akiyutaka 7 times in succession, the variants maintained their original pathogenicity. The parasexual recombinants produced more conidia and larger lesions on the host than did parent TH77-1, but fewer conidia and smaller lesions than parent NAO-02. Disease development on plants inoculated with either variant or a parental isolate was also midway between the disease caused by each of the parents in a field containing a single cultivar, but the variant caused the most severe disease in a plot with a mixture of cultivars. These results suggest that parasexual recombination produces variation in fitness.

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Section: Introductionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Fitness Characters in Parasexual Recombinants of the Rice Blast Fungus, Pyricularia oryzae

Noguchi

Yasuda

Fujita

2007

JARQ

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“…Heterokaryosis and a parasexual cycle have been reported in other filamentous fungi 3,5,6,8,13,14,17,18,22,25 , and parasexual recombination has proven to be a valuable tool in genetic analyses of filamentous fungi, particularly imperfect fungi. For example, the placement of adenine, lysine and white conidium gene markers in the same linkage group in Aspergillus niger was revealed through the parasexual cycle 10 .…”

Section: Discussionmentioning

confidence: 99%

“…Although many comprehensive studies on parasexuality in the rice blast fungus have been conducted 3,5,6,8,13,14,20,22,25 , little direct evidence exists of the parasexual cycle in nature. Nevertheless, parasexuality must be able to make a considerable contribution to genetic diversity in field populations of the rice blast fungus based on some observations 2,25 .…”

Section: Discussionmentioning

confidence: 99%

“…While numerous studies on the parasexual cycle have been conducted in the rice blast fungus 3,5,6,8,13,14,22,25 , no genetic analyses have been performed on parasexual recombinants that included avirulence genes. Therefore, we carried out a genetic study after obtaining parasexual recombinants from co-cultures of two different antibiotic-resistant isolates of the fungus: isolate Y90-71BI derived from introducing plasmid pBARKS1, a bialaphos (BI)-resistance vector that contains the Ignite/Basta-resistance (bar) gene 1,16 , into isolate Y90-71, and isolate 3514-R-2BS derived from introducing plasmid pBF101, a blasticidin S (BS)-resistance vector that contains the blasticidin S deaminase gene (BSD) 9 , into isolate 3514-R-2 15 .…”

Section: Introductionmentioning

confidence: 99%

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