Inter‐Individual Variability in Insulin Response after Grape Pomace Supplementation in Subjects at High Cardiometabolic Risk: Role of Microbiota and miRNA

Ramos-Romero, Sara; Léniz, Asier; Martínez-Maqueda, Daniel; Amézqueta, Susana; Fernández‐Quintela, Alfredo; Hereu, Mercè; Torres, Josep Luís; Portillo, Marı́a P.; Pérez‐Jiménez, Jara

doi:10.1002/mnfr.202000113

Cited by 21 publications

(22 citation statements)

References 59 publications

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“…First, First, De Araujo et al [ 40 ] defined short-term and long-term HIIT programs in function of their duration (<6 weeks and ≥12 weeks, respectively). Furthermore, nutritional supplementation-induced gut microbiota modulation is observed after at least 2 weeks of treatment and generally lasts less than 24 weeks [ 41 , 42 , 43 , 44 ]. Therefore, 12 weeks seemed a good compromise to obtain significant body composition and gut microbiota alterations, and was recently chosen also by Plissonneau et al [ 45 ] with the same HIIT protocol.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the metabolic adaptations to nutritional interventions also show variability, particularly upon exposure to polyphenolic compounds [ 80 ]. Moreover, metabolic adaptations induced by nutritional interventions might be influenced by the gut microbiota composition before the supplementation/diet [ 44 , 81 ]. Altogether, these findings suggest that the greater results of the HIIT + T63 combination on body composition and glucose metabolism in our study might be explained by its effect on the gut microbiota composition.…”

Section: Discussionmentioning

confidence: 99%

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The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

Dupuit

Chavanelle²,

Chassaing

et al. 2021

Nutrients

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Obesity and prediabetes are the two strongest risk factors of type 2 diabetes. It has been reported that TOTUM-63, a polyphenol-rich plant extract, has beneficial effects on body weight (BW) and insulin resistance in mice fed a high fat diet (HFD). The study aim was to determine whether high-intensity interval training (HIIT) and/or TOTUM-63 supplementation improved body composition and glycemic control and gut microbiota composition in a Western diet-induced obesity rat model. Wistar rats received a standard diet (CTRL; control; n = 12) or HFD (HFD; n = 48) for 16 weeks. Then, HFD rats were divided in four groups: HFD, HFD + TOTUM-63 (T63), HFD + HIIT (HIIT), and HFD + HIIT +T63 (HIIT + T63). Training was performed 4 days/week for 12 weeks. TOTUM-63 was included in diet composition (2%). The HIIT + T63 combination significantly limited BW gain, without any energy intake modulation, and improved glycemic control. BW variation was correlated with increased α-diversity of the colon mucosa microbiota in the HIIT + T63 group. Moreover, the relative abundance of Anaeroplasma, Christensenellaceae and Oscillospira was higher in the HIIT + T63 group. Altogether, these results suggest that the HIIT and TOTUM-63 combination could be proposed for the management of obesity and prediabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

Dupuit

Chavanelle²,

Chassaing

et al. 2021

Nutrients

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“…and this was repeated every other day thereafter for 10 weeks. DATS was synthesized using Bunte salts [25,45] and purified with high-performance liquid chromatography following our previously described method. [4] DATS was precisely administered at Zeitgeber time 0.…”

Section: Methodsmentioning

confidence: 99%

“…Nutrients and related bioactive compounds including fatty acids, resveratrol, curcumin, quercetin, and catechins modulate miRNA activity and influence mRNA expression. [21][22][23][24][25][26] DATS also has a miRNAmodulating effect. DATS promotes neovasculogenesis through the regulation of miR-211 in human endothelial progenitor cells [27] ; DATS also inhibits cell proliferation, invasion, and angiogenesis through the regulation of miR-34a, miR-143, miR-145, and miR-200b/c in osteosarcoma cells.…”

Section: Introductionmentioning

confidence: 99%

Diallyl Trisulfide Prevents Obesity and Decreases miRNA‐335 Expression in Adipose Tissue in a Diet‐Induced Obesity Rat Model

Miura

Ikeda

Abe

et al. 2021

Molecular Nutrition Food Res

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Scope: Diallyl trisulfide (DATS), an organosulfur compound generates in crushed garlic, has various beneficial health effects. A growing body of evidence indicates that miRNAs are involved in the pathology of lifestyle diseases including obesity. The anti-obesogenic effect of garlic is previously reported; however, the effects of DATS on obesity, and the relationship between garlic compounds and the involvement of miRNA remains unclear. Here, the anti-obesogenic activity of DATS and the potential role of miRNA in a diet-induced obesity rat model are investigated. Methods and Results: Oral administration of DATS suppressed body and white adipose tissue (WAT) weight gain in rats fed a high-fat diet compared with vehicle-administered rats. DATS lowered the plasma and liver triglyceride levels in obese rats, and decreased lipogenic mRNA levels including those of Srebp1c, Fasn, and Scd1 in the liver. DATS also suppressed de novo lipogenesis in the liver. Transcriptomic analyses of miRNA and mRNA in the epididymal WAT of obese rats using microarrays revealed that DATS decreased miRNA-335 expression and normalized the obesity-related mRNA transcriptomic signatures in epididymal WAT. Conclusion: The potent anti-obesogenic effects of DATS and its possible mechanism of action was clearly demonstrated in this study.

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“…For this purpose, volunteers were provided with a detailed list of foods to be avoided. At the end of the experimental period, 23 participants were classified as responder subjects and 26 as non-responders, based on a decrease in fasting insulin ≥10% [ 21 ]. Although the level of glucose remained unchanged, a strong reduction in insulin level was found in responder subjects (−40%) and, as a consequence, HOMA-IR was also significantly reduced [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

Léniz

Martínez-Maqueda

Quintela

et al. 2021

Foods

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MicroRNAs (miRNAs) represent important tools in medicine and nutrition as new biomarkers, and can act as mediators of nutritional and pharmacological interventions. The aim of the present study was to analyse the effect of grape pomace supplementation on the expression of seven selected miRNAs and their potential relationship with the observed positive effect on glycaemic control, in order to shed light on the mechanism underlying the beneficial effect of this dietary intervention. For this purpose, plasma samples were obtained from 49 subjects with metabolic syndrome. After supplementation with grape pomace (6 weeks), these subjects were categorised as responders (n = 23) or non-responders (n = 26) according to the changes in their fasting insulin rate. MiRNA expression at baseline and at the end of the supplementation was analysed by RT-PCR, and the MiRecords Database was used to identify potential target genes for the studied miRNAs. The increase observed in miR-23a in the whole cohort was present in both subgroups of participants. The increase in miR-181a was significant among non-responders but not responders. The decrease in miR-30c and miR-222 was found in the responders, but not in the non-responders. No changes were observed in miR-10a, miR-151a, miR-181a, and miR-let-7a expressions. After analysing these results, a potential involvement of the reduced expression of miR-30c and miR-222, two microRNAs associated with insulin resistance and diabetes, in the improvement of glycaemic control produced by grape pomace administration, can be proposed. Further research is needed to confirm the involvement of glycolytic enzymes, PI3K, AMPK, and IRS-1 in the effect of grape pomace, as suggested by the changes induced in microRNAs.

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Inter‐Individual Variability in Insulin Response after Grape Pomace Supplementation in Subjects at High Cardiometabolic Risk: Role of Microbiota and miRNA

Cited by 21 publications

References 59 publications

The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

Diallyl Trisulfide Prevents Obesity and Decreases miRNA‐335 Expression in Adipose Tissue in a Diet‐Induced Obesity Rat Model

Potential Relationship between the Changes in Circulating microRNAs and the Improvement in Glycaemic Control Induced by Grape Pomace Supplementation

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