Inter-comparison of stable iron, copper and zinc isotopic compositions in six reference materials of biological origin

Sauzéat, Lucie; Costas‐Rodríguez, Marta; Albalat, Emmanuelle; Mattielli, Nadine; Vanhaecke, Frank; Balter, Vincent

doi:10.1016/j.talanta.2020.121576

Cited by 16 publications

(18 citation statements)

References 67 publications

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“…The two full replicates measured for NIST1515, which underwent identical procedures in separate beakers from the digestion process, present identical values (−0.71 ± 0.06%0 and −0.71 ± 0.04%0). Two samples (ERC-CE464, tuna fish and ERM-BB186, pig kidney) had previously been analysed ( 51 , 52 ) and our data are in very good agreement with previously reported values ( Figure 2 ). The isotopic composition of BHVO-2 is within error of previously published values [0.17 ± 0.03%0 for δ 57 Fe and 0.10 ± 0.04%0 for δ 56 Fe, e.g., ( 56 )].…”

Section: Resultssupporting

confidence: 92%

“…Quality control of stable isotopic measurements necessitates well-characterised and widely available reference materials, with a matrix similar to the matrix of the sample of interest. Although research fields such as geochemistry benefit from numerous extensively characterised international reference rock materials, metal isotope compositions of certified reference materials of biological origin are very scarce [e.g., ( 50 – 52 )]. In this context, we have determined the Fe isotope composition in nine widely available international biological standards.…”

Section: Introductionmentioning

confidence: 99%

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Iron Isotopic Composition of Biological Standards Relevant to Medical and Biological Applications

et al. 2021

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Iron isotopes are fractionated by multiple biological processes, which offers a novel opportunity to study iron homeostasis. The determination of Fe isotope composition in biological samples necessitates certified biological reference materials with known Fe isotopic signature in order to properly assess external reproducibility and data quality between laboratories. We report the most comprehensive study on the Fe isotopic composition for widely available international biological reference materials. They consist of different terrestrial and marine animal organs (bovine, porcine, tuna, and mussel) as well as apple leaves and human hair (ERC-CE464, NIST1515, ERM-DB001, ERM-BB186, ERM-BB184, ERM-CE196, BCR668, ERM-BB185, ERM-BB124). Previously measured Fe isotopic compositions were available for only two of these reference materials (ERC-CE464 tuna fish and ERM-BB186 pig kidney) and these literature data are in excellent agreement with our data. The Fe isotopic ratios are reported as the permil deviation of the 56Fe/54Fe ratio from the IRMM-014 standard. All reference materials present δ56Fe ranging from −2.27 to −0.35%0. Combined with existing data, our results suggest that animal models could provide useful analogues of the human body regarding the metabolic pathways affecting Fe isotopes, with many potential applications to medicine.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Iron Isotopic Composition of Biological Standards Relevant to Medical and Biological Applications

et al. 2021

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“…Iron was isolated by ion-exchange chromatography as described in details in previous study (27). Briefly, the dried residue was taken up in HCl 6N and evaporated to dryness twice to guarantee total removal of remaining nitrate from the digestion procedure.…”

Section: Elemental and Isotopic Analysismentioning

confidence: 99%

“…Iron solutions were diluted to 0.9 mg/L and doped with Ni at the same concentration to monitor and correct for instrumental mass fractionation. The instrument settings and analytical conditions are described in previous work (27). All the Fe isotope compositions are given in the delta annotation (expressed in ‰) and reported relative to the international isotopic standard NIST IRMM-014 with the following formula:…”

Section: Elemental and Isotopic Analysismentioning

confidence: 99%

Hfe Gene Knock-Out in a Mouse Model of Hereditary Hemochromatosis Affects Bodily Iron Isotope Compositions

Albalat

Cavey²,

Leroyer³

et al. 2021

Front. Med.

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Hereditary hemochromatosis is a genetic iron overload disease related to a mutation within the HFE gene that controls the expression of hepcidin, the master regulator of systemic iron metabolism. The natural stable iron isotope composition in whole blood of control subjects is different from that of hemochromatosis patients and is sensitive to the amount of total iron removed by the phlebotomy treatment. The use of stable isotopes to unravel the pathological mechanisms of iron overload diseases is promising but hampered by the lack of data in organs involved in the iron metabolism. Here, we use Hfe−/− mice, a model of hereditary hemochromatosis, to study the impact of the knock-out on iron isotope compositions of erythrocytes, spleen and liver. Iron concentration increases in liver and red blood cells of Hfe−/− mice compared to controls. The iron stable isotope composition also increases in liver and erythrocytes, consistent with a preferential accumulation of iron heavy isotopes in Hfe−/− mice. In contrast, no difference in the iron concentration nor isotope composition is observed in spleen of Hfe−/− and control mice. Our results in mice suggest that the observed increase of whole blood isotope composition in hemochromatosis human patients does not originate from, but is aggravated by, bloodletting. The subsequent rapid increase of whole blood iron isotope composition of treated hemochromatosis patients is rather due to the release of hepatic heavy isotope-enriched iron than augmented iron dietary absorption. Further research is required to uncover the iron light isotope component that needs to balance the accumulation of hepatic iron heavy isotope, and to better understand the iron isotope fractionation associated to metabolism dysregulation during hereditary hemochromatosis.

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“…We first evaluated the quality of the dissolution method by comparing the results of element concentration (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) in all the reference materials (SRM‐1577c, BCR‐383, BCR‐380R, ERM‐CE464, DORM‐2, DORM‐4, TORT‐3, and FBS), with those published in Sauzéat et al 16 Our data strongly correlate with certified values ( R = 0.99; P ‐value <2.2 × 10 −16 ; n = 37) according to a linear regression with a slope of 0.96 (±0.01) and an offset at origin of –163 (±742), showing that the dissolution step was efficient at solubilizing all the elements. We also calculated the yields of Mg for the whole elution method and found quantitative yields (from 80% to 106%) for all the reference materials (SRM‐1577c, BCR‐383, BCR‐380R, ERM‐CE464, DORM‐2, DORM‐4, and TORT‐3), except for FBS (where the Mg yield was 77%).…”

Section: Resultsmentioning

confidence: 99%

Determination of magnesium isotopic ratios of biological reference materials via multi‐collector inductively coupled plasma mass spectrometry

Goff

Albalat

Dosseto

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale Despite a wide range of potential applications, magnesium (Mg) isotope composition has been so far sparsely measured in reference materials with a biological matrix, which is important for the quality control of the results. We describe a method enabling the chemical separation of Mg in geological and biological materials and the determination of its stable isotope composition. Methods Different geological (BHVO‐1, BHVO‐2, BCR‐1, and IAPSO) and biological (SRM‐1577c, BCR‐383, BCR380R, ERM‐CE464, DORM‐2, DORM‐4, TORT‐3, and FBS) reference materials were used to test the performance of a new sample preparation procedure for Mg isotopic analysis. The procedure consisted of a simple three‐stage elution method to separate Mg from the matrix. Mg isotopic analyses were performed in two different laboratories and with three different multi‐collector inductively coupled plasma mass spectrometry instruments. Results The biological reference materials show a wide range of δ26Mg values (relative to DSM3 standard), spanning over 2‰, from 0.52 ± 0.29‰ (2SD, n = 7) in bovine liver (SRM‐1577c) to −1.45 ± 0.20‰ (2SD, n = 5) in tuna fish (ERM‐CE464), with an external precision of 0.03‰ (2SD, n = 85). Conclusions This study indicates that isotopic measurements of Mg in biological reference materials show good performance, with the results being within the accepted range. We confirmed that δ26Mg values in liver are the most positive of all biological materials reported so far.

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Inter-comparison of stable iron, copper and zinc isotopic compositions in six reference materials of biological origin

Cited by 16 publications

References 67 publications

Iron Isotopic Composition of Biological Standards Relevant to Medical and Biological Applications

Iron Isotopic Composition of Biological Standards Relevant to Medical and Biological Applications

Hfe Gene Knock-Out in a Mouse Model of Hereditary Hemochromatosis Affects Bodily Iron Isotope Compositions

Determination of magnesium isotopic ratios of biological reference materials via multi‐collector inductively coupled plasma mass spectrometry

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