Inter- and intraspecific variation of chloroplast mini- and microsatellites DNA in the four closed related Acacia species

Abstract

Mini-and microsatellites of four Acacia species, A. aulacocarpa, A. auriculiformis, A. crassicarpa and A. mangium were investigated on four non-coding regions of cpDNA, the intron of trnL, and the intergenic spacers of trnL -trnP, trnD -trnY, and trnP -trnW. Nine single base substitutions and six informative miniand microsatellites were detected in the the four cpDNA non-coding regions. Based on the substitutions and mini-and microsatellites, ten cpDNA haplotypes (A -J) could be distinguished. Acacia auriculiformis possessed fi ve haplotypes, A. aulacocarpa, four haplotypes, and A. crassicarpa, three haplotypes. All samples of A. mangium possessed the same haplotype. Mini-and microsatellites recognized in this study can be used for species identifi cation of the four Acacia species. The ten haplotypes could divided the four species into 2 groups, A. aulacocarpa-A.crassicarpa group and A. auriculiformis-A. mangium group. By developing the PCR-based markers based on the sequence information, many experiments can be carried out for the Acacia improvement programs.