Inter- and intraspecific hypervariability in interstitial telomeric-like repeats (TTTAGGG)n in Anacyclus (Asteraceae)

Rosato, Marcela; Álvarez, Inés; Feliner, Gonzalo Nieto; Rosselló, Josep A.

doi:10.1093/aob/mcy079

Cited by 15 publications

(36 citation statements)

References 51 publications

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“…The majority of the species of the Anthemideae tribe have basic chromosome number x = 9, while x = 8-based species have been found in the Artemisia genus suggesting that Robertsonian translocations might have participated in the evolution of some species of this tribe (Garcia et al, 2006; Mas de Xaxars et al, 2016). However, this hypothesis of ITRs origin in the Anthemidae is not the most parsimonious as there was no change in chromosome number neither in Anacyclus , nor in Tanacetum (both at the ploidy level) (Olanj et al, 2015; Rosato et al, 2018). On the other hand, Anacyclus species are characterized by striking variability of ITR sites (Rosato et al, 2018).…”

Section: Discussionmentioning

confidence: 97%

“…However, this hypothesis of ITRs origin in the Anthemidae is not the most parsimonious as there was no change in chromosome number neither in Anacyclus , nor in Tanacetum (both at the ploidy level) (Olanj et al, 2015; Rosato et al, 2018). On the other hand, Anacyclus species are characterized by striking variability of ITR sites (Rosato et al, 2018). Furthermore, in T. cinerariifolium ITRs are present not only in pericentric regions but also in interstitial positions, while in the sister genus Anacyclus ITRs are present exclusively in the interstitial positions (this study and Rosato et al, 2018).…”

Section: Discussionmentioning

confidence: 97%

“…In contrast, no ITRs were detected in four species of Argyranthemum (Borgen et al, 2003), a genus also belonging in the tribe Anthemideae, but distantly related to Tanacetum (Oberprieler et al, 2009). In order to explain scattered distribution of ITRs among the Anthemideae, Rosato et al (2018) proposed a hypothesis that ITRs originated early in the diversification of the Eurasian clade of Anthemideae (Oberprieler et al, 2009) and were followed by complex and recurrent cycles of amplification, genomic spread and contraction.…”

Section: Discussionmentioning

confidence: 99%

“…Their origin largely remains unknown, however, it was suggested that ITRs might originate from ancestral intrachromosomal rearrangements involving telomeric regions (inversions and fusions), unequal crossing-over or from the repair of DSBs. ITRs have been reported in various organisms, including vertebrates (Meyne et al, 1990) and plants (Cox et al, 1993; Fuchs et al, 1995; Rosato et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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The Repetitive DNA Composition in the Natural Pesticide Producer Tanacetum cinerariifolium: Interindividual Variation of Subtelomeric Tandem Repeats

et al. 2019

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Dalmatian pyrethrum ( Tanacetum cinerariifolium (Trevir.) Sch. Bip.), a plant species endemic to the east Adriatic coast, is used worldwide for production of the organic insecticide, pyrethrin. Most studies concerning Dalmatian pyrethrum have focused on its morphological and biochemical traits relevant for breeding. However, little is known about the chromosomal evolution and genome organization of this species. Our study aims are to identify, classify, and characterize repetitive DNA in the T. cinerariifolium genome using clustering analysis of a low coverage genomic dataset. Repetitive DNA represents about 71.63% of the genome. T. cinerariifolium exhibits linked 5S and 35S rDNA configuration (L-type). FISH reveals amplification of interstitial telomeric repeats (ITRs) in T. cinerariifolium . Of the three newly identified satellite DNA families, TcSAT1 and TcSAT2 are located subterminally on most of T. cinerariifolium chromosomes, while TcSAT3 family is located intercalary within the longer arm of two chromosome pairs. FISH reveals high levels of polymorphism of the TcSAT1 and TcSAT2 sites by comparative screening of 28 individuals. TcSAT2 is more variable than TcSAT1 regarding the number and position of FISH signals. Altogether, our data highlights the dynamic nature of DNA sequences associated with subtelomeres in T. cinerariifolium and suggests that subtelomeres represent one of the most dynamic and rapidly evolving regions in eukaryotic genomes.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Repetitive DNA Composition in the Natural Pesticide Producer Tanacetum cinerariifolium: Interindividual Variation of Subtelomeric Tandem Repeats

et al. 2019

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“…Moreover, some genotoxic compounds were shown to induce ITSs amplification and accumulation [246]. Hypervariability of ITSs within a single species was recently reported in plants [284]. Telomeric repeats are highly recombinogenic in yeast [293], rodents [294,295,296] and humans [297].…”

Section: Interstitial Telomeric Sequences (Itss)mentioning

confidence: 99%

At the Beginning of the End and in the Middle of the Beginning: Structure and Maintenance of Telomeric DNA Repeats and Interstitial Telomeric Sequences

Aksenova

Mirkin

2019

Genes

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Tandem DNA repeats derived from the ancestral (TTAGGG)n run were first detected at chromosome ends of the majority of living organisms, hence the name telomeric DNA repeats. Subsequently, it has become clear that telomeric motifs are also present within chromosomes, and they were suitably called interstitial telomeric sequences (ITSs). It is well known that telomeric DNA repeats play a key role in chromosome stability, preventing end-to-end fusions and precluding the recurrent DNA loss during replication. Recent data suggest that ITSs are also important genomic elements as they confer its karyotype plasticity. In fact, ITSs appeared to be among the most unstable microsatellite sequences as they are highly length polymorphic and can trigger chromosomal fragility and gross chromosomal rearrangements. Importantly, mechanisms responsible for their instability appear to be similar to the mechanisms that maintain the length of genuine telomeres. This review compares the mechanisms of maintenance and dynamic properties of telomeric repeats and ITSs and discusses the implications of these dynamics on genome stability.

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Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna

Waminal

Nguyen

et al. 2021

Genes Genom

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Background DNA tandem repeats (TRs) are often abundant and occupy discrete regions in eukaryotic genomes. These TRs often cause or generate chromosomal rearrangements, which, in turn, drive chromosome evolution and speciation. Tracing the chromosomal distribution of TRs could therefore provide insights into the chromosome dynamics and speciation among closely related taxa. The basic chromosome number in the genus Senna is 2n = 28, but dysploid species like Senna tora have also been observed. Objective To understand the dynamics of these TRs and their impact on S. tora dysploidization. Methods We performed a comparative fluorescence in situ hybridization (FISH) analysis among nine closely related Senna species and compared the chromosomal distribution of these repeats from a cytotaxonomic perspective by using the ITS1-5.8S-ITS2 sequence to infer phylogenetic relationships. Results Of the nine S. tora TRs, two did not show any FISH signal whereas seven TRs showed similar and contrasting patterns to other Senna species. StoTR01_86, which was localized in the pericentromeric regions in all S. tora, but not at the nucleolar organizer region (NOR) site, was colocalized at the NOR site in all species except in S. siamea. StoTR02_7_tel was mostly localized at chromosome termini, but some species had an interstitial telomeric repeat in a few chromosomes. StoTR05_180 was distributed in the subtelomeric region in most species and was highly amplified in the pericentromeric region in some species. StoTR06_159 was either absent or colocalized in the NOR site in some species, and StoIGS_463, which was localized at the NOR site in S. tora, was either absent or localized at the subtelomeric or pericentromeric regions in other species. Conclusions These data suggest that TRs play important roles in S. tora dysploidy and suggest the involvement of 45S rDNA intergenic spacers in “carrying” repeats during genome reshuffling.

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Inter- and intraspecific hypervariability in interstitial telomeric-like repeats (TTTAGGG)n in Anacyclus (Asteraceae)

Cited by 15 publications

References 51 publications

The Repetitive DNA Composition in the Natural Pesticide Producer Tanacetum cinerariifolium: Interindividual Variation of Subtelomeric Tandem Repeats

The Repetitive DNA Composition in the Natural Pesticide Producer Tanacetum cinerariifolium: Interindividual Variation of Subtelomeric Tandem Repeats

At the Beginning of the End and in the Middle of the Beginning: Structure and Maintenance of Telomeric DNA Repeats and Interstitial Telomeric Sequences

Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna

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