Inter- and intraspecific evolutionary relationships of the rice frog Rana limnocharis and the allied species R. cancrivora inferred from crossing experiments and mitochondrial DNA sequences of the 12S and 16S rRNA genes

Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

“…The primers were also used for DNA sequencing. We used the following additional primers for sequencing when necessary: F51 and R51 (Sumida et al, 2002), 16S_R530 and 16S_R723 (Hasan et al, 2012a), R1051 (5´-ATGTTTTTGGTAAACAGGCGGG-3´), FR60 (5´-AAGCAAGTCGTAACATGGTA-3´), 16S_Rev1_SMA (5´-GGTTATTGTTAAGCTTTAACGC-3´), and 16S_Rev2_ CANCRI (5´-GAGAAATTAAGCTTTAACGC-3´). The length of the resultant 16S fragments varied from 1287 to 1354 bp between taxa.…”

Complete nucleotide sequence and gene arrangement of the mitochondrial genome of the crab-eating frog Fejervarya cancrivora and evolutionary implications

“…In many cases, the members of this genus possess relatively few diagnostics; thus, the genus becomes one of the most problematic anuran taxa taxonomically (e.g., Kotaki et al, 2008). Indeed, F. cancrivora has occasionally been mistaken as its closely related species, F. limnocharis (e.g., Inger, 1954; but see Sumida et al, 2002). For the above reasons, usable molecular information, such as mt genomic data, and detailed phylogenetic analyses on this taxon have been expected.…”

Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident

“…Total genomic DNA of each individual was extracted from clipped toes using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. PCR amplification of the partial 16S rRNA gene was performed in a 20-mL volume containing 10-mL of EmeraldAmp MAX PCR Master Mix (TaKaRa), 1 mL of genomic DNA solution, and 2 mL of the 10 mM primer pair F51 (5 0 -CCCGCCTGTTTACCAAAAACAT-3 0 ) and R51 (5 0 -GGTCTGAACTCAGATCACGTA-3 0 ) (Sumida et al, 2002). Thermal cycling was performed under the following conditions: 95 C for 3 min, followed by 35 cycles of 95 C for 30 s, 55 C for 30 s, and 72 C for 30 s. PCR products were digested with SpeI and HphI separately and visualized by electrophoresis on 2.0% agarose gel.…”