Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity

Meij, Anne van der; Willemse, Joost; Schneijderberg, Martinus; Geurts, René; Raaijmakers, Jos M.; Wezel, Gilles P. van

doi:10.1007/s10482-018-1014-z

Cited by 58 publications

(38 citation statements)

References 52 publications

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“…Particularly, colonization of A. thaliana roots by Streptomyces lividans was shown to provide protection of the former against fungal infection [8]. Recently A. thaliana colonization by endophytic streptomycetes has been reported as well as their responses to plant hormones [9]. These and other studies support the assumption that Streptomyces spp.…”

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confidence: 63%

Microbe-plant interactions between Streptomyces and model agricultural plants – Hordeum vulgare and Lycopersicon esculentum (Microtom)

Mutenko¹,

Ostash²,

Rothballer³

et al. 2019

Fakt. eksp. evol. org.

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Aim. Microbe-plant interactions (MPI) constitute an important aspect of ecology because of their significant influence on plant’s ability to withstand abiotic stress and infection. In comparison to proteobacteria and bacilli, the roles of streptomycetes in MPI remain poorly studied. Here, we elucidate some aspects of MPI between two model plant species, Hordeum vulgare and Lycopersicon esculentum, and several strains of Streptomyces lividans 1326 and S. ghanaensis ATCC14672. Methods. Microbiology, microscopy and molecular genetics were combined to reveal the MPI. Results. We demonstrate the colonization of H. vulgare and L. esculentum roots by different strains of S. ghanaensis deficient in production of either the antibiotic moenomycin or signaling molecule of the γ-butyrolactone type. The treatment of H. vulgare seeds with S. lividans spores increased the root biomass. Plants treated with 1,4-butyrolactone had no positive influence on plants, at milimolar concentrations this compound inhibited the root and shoot growth of L. esculentum. Conclusions. Roots of two mono- and dicot plants are colonized by Streptomyces; reporter gene uidA is useful to monitor the colonization. Under our experimental conditions the ability to colonize plants by streptomycetes was not affected by the deficiency in antibiotic or butenolide production. Keywords: Streptomyces ghanaensis, moenomycinA, low-molecular weight signal compounds, root colonization.

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confidence: 63%

Microbe-plant interactions between Streptomyces and model agricultural plants – Hordeum vulgare and Lycopersicon esculentum (Microtom)

Mutenko¹,

Ostash²,

Rothballer³

et al. 2019

Fakt. eksp. evol. org.

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“…Agrobacterium will naturally colonize plants and form benign bio-films on root surfaces ( Heindl et al., 2014 ) and it is generally accepted that antimicrobial substances released at the wound can limit colonization ( Akinsulire et al., 2008 ; Taye et al., 2011 ; Meij et al., 2018 ). We show here that Agrobacterium cells are able to colonize wounded mature tobacco explants ( Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

Mapping Reaction-Diffusion Networks at the Plant Wound Site With Pathogens

et al. 2020

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The rich collection of microbes colonizing the plant root making up the rhizosphere function as a multigenomic organ for nutrient distribution. The extent to which its dynamic mutualistic cellular order depends on morphogenic signaling, while likely, remains unknown. We have shown that reaction-diffusion chemical networks constructed with model plant and bacterial metabolites can mimic processes ranging from oxidative burst kinetics to traveling waves and extracellular stationary state reaction-diffusion networks for spatiotemporal ordering of the rhizosphere. Plant parasites and pathogens can be limited by host attachment require dynamic informational networks and continue to provide insight into what controls the rhizosphere. Here we take advantage of Agrobacterium tumefaciens, a plant pathogen with a gated receptor that requires simultaneous perception of two plant metabolites. Genetic manipulations have created receptors allowing each metabolite concentration to be correlated with pathogen behavior. The development of the florescent strains used here provide initial maps of the reactiondiffusion dynamics existing in the rhizosphere, revealing significant differences in the signaling landscape of host and non-host plants before and after wounding, specifically highlighting networks that may inform rhizosphere organization.

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“…As soil-dwelling bacteria, filamentous actinomycetes are often found in association with eukaryotic hosts, including plants 30 . The root systems of plants are highly dynamic in their oxygen availability and can, depending on their abiotic influences, transition to an anoxic state 31 .…”

Section: Discussionmentioning

confidence: 99%

Formation of wall-less cells inKitasatospora viridifaciensrequires cytoskeletal protein FilP in oxygen-limiting conditions

Ultee

Briegel

Claessen

2020

Preprint

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13The cell wall is considered an essential component for bacterial survival, providing structural support 14 and protection from environmental insults. Under normal growth conditions, filamentous 15 actinobacteria insert new cell wall material at the hyphal tips regulated by the coordinated activity of 16 cytoskeletal proteins and cell wall biosynthetic enzymes. Despite the importance of the cell wall, some 17 filamentous actinobacteria can produce wall-deficient S-cells upon prolonged exposure to 18 hyperosmotic stress. Here we performed cryo-electron tomography and live cell imaging to further 19 characterize S-cell extrusion in Kitasatospora viridifaciens. We show that exposure to hyperosmotic 20 stress leads to DNA compaction, membrane and S-cell extrusion and thinning of the cell wall at 21 hyphal tips. Additionally, we find that the extrusion of S-cells is abolished in a cytoskeletal mutant 22 strain that lacks the intermediate filament-like protein FilP. Furthermore, micro-aerobic culturing 23 promotes the formation of S-cells in the wild-type, but the limited oxygen still impedes S-cell 24 formation in the ΔfilP mutant. These results demonstrate that S-cell formation is stimulated by 25 oxygen-limiting conditions and dependent on the presence of an intact cytoskeleton. 26 27

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Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity

Cited by 58 publications

References 52 publications

Microbe-plant interactions between Streptomyces and model agricultural plants – Hordeum vulgare and Lycopersicon esculentum (Microtom)

Microbe-plant interactions between Streptomyces and model agricultural plants – Hordeum vulgare and Lycopersicon esculentum (Microtom)

Mapping Reaction-Diffusion Networks at the Plant Wound Site With Pathogens

Formation of wall-less cells inKitasatospora viridifaciensrequires cytoskeletal protein FilP in oxygen-limiting conditions

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