Intensity Enhanced Cerenkov Luminescence Imaging Using Terbium-Doped Gd₂O₂S Microparticles

Abstract

Weak intensity and poor penetration depth are two big obstacles toward clinical use of Cerenkov luminescence imaging (CLI). In this proof-of-concept study, we overcame these limitations by using lanthanides-based radioluminescent microparticles (RLMPs), called terbium doped Gd2O2S. The characterization experiment showed that the emission excited by Cerenkov luminescence can be neglected whereas the spectrum experiment demonstrated that the RLMPs can actually be excited by γ-rays. A series of in vitro experiments demonstrated that RLMPs significantly improve the intensity and the penetration capacity of CLI, which has been extended to as deep as 15 mm. In vivo pseudotumor study further prove the huge potential of this enhancement strategy for Cerenkov luminescence imaging in living animal studies.

Intrinsically Zirconium-89 Labeled Gd2 O2 S:Eu Nanoprobes for In Vivo Positron Emission Tomography and Gamma-Ray-Induced Radioluminescence Imaging

“…Radioluminescence imaging is an emerging innovative optical imaging modality that utilizes ionizing radiation of high energy rays (such as X‐rays, gamma rays or beta particles) to excite nanophorphors to visualize biological features with an improved signal‐to‐noise ratio and a deeper tissue penetration depth (when compared with conventional optical imaging approaches) . Radioluminescence nanophosphors have recently shown great promise for biomedical imaging due to their unique optical properties, including exceptional photochemical stability, tunable emission spectrum with large Stokes shifts, negligible photobleaching, and bright radioluminescence .…”

“…Radioluminescence imaging is an emerging innovative optical imaging modality that utilizes ionizing radiation of high energy rays (such as X-rays, gamma rays or beta particles) to excite nanophorphors to visualize biological features with an improved signal-to-noise ratio and a deeper tissue penetration depth (when compared with conventional optical imaging approaches). [1][2][3][4][5][6] Radioluminescence nanophosphors have recently shown great promise for biomedical imaging small 2016, 12, No. 21, 2872-2876 saw a slight optical quenching effect and reduced fl uorescent intensity in the water soluble Gd 2 O 2 S:Eu@PEG nanoparticles (Figure 1 c, inset), possibly due to the optical quenching in aqueous solution.…”

Scintillating Nanoparticles as Energy Mediators for Enhanced Photodynamic Therapy

“…131 Terbium doped Gd 2 O 2 S (Gd 2 O 2 S:Tb) nanomaterials were reported as X-ray nanophosphors and synthesized using complex precipitation methods. 132 In this study, Gd 2 O 2 S:Tb was activated by 18 FDG, resulting in the enhancement of luminescence excited by γ-rays.…”

Radionuclide‐Activated Nanomaterials and Their Biomedical Applications

“…In another proof-of-concept study,C ao et al [55] investigated the scintillation of terbium-doped Gd 2 O 2 Se xcited by 18 F-FDG g rays.Results demonstrated that 1) there is asaturation limitation for exciting ac ertain amount of NPS, 2) there is an exponential attenuation of the radioluminescence intensity with the increase in the distance,a nd 3) the intensity of the radioluminescence present in the site containing the 18 F-FDG combined with the NPs was significantly greater than that found for the radionuclide alone.…”