Intensification of total phenolic compounds extraction from Azadirachta indica (Neem) leaves by ultrasound

Shewale, Sandeep P; Kapadia, Miraj; Rathod, Virendra K.

doi:10.1016/j.cep.2022.109099

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…The TPC and flavonoid contents were used to characterize the obtained extracts. The results presented in Figure 1 are in accordance with Arsul et al [33], which also reported lower TFC and TPC in the HEs compared to the EAE. Arsul et al [33] found the TFC (mg QE/g extract) of HEs ranging from 5.83 ± 0.10 (red varieties of bajakah) to 20.07 ± 1.26 (white varieties of bajakah) and TPC (mg GAE/g extract) from 84.57 ± 1.26 (red varieties of bajakah) to 172.49 ± 3.35 (white varieties of bajakah).…”

Section: Discussionsupporting

confidence: 90%

“…The results presented in Figure 1 are in accordance with Arsul et al [33], which also reported lower TFC and TPC in the HEs compared to the EAE. Arsul et al [33] found the TFC (mg QE/g extract) of HEs ranging from 5.83 ± 0.10 (red varieties of bajakah) to 20.07 ± 1.26 (white varieties of bajakah) and TPC (mg GAE/g extract) from 84.57 ± 1.26 (red varieties of bajakah) to 172.49 ± 3.35 (white varieties of bajakah). The TFC (mg QE/g extract) of EAEs were ranging from 15.17 ± 0.17 (red varieties of bajakah) to 34.25 ± 0.36 (white varieties of bajakah) and TPC (mg GAE/g extract) from 217.98 ± 7.95 (red varieties of bajakah) to 653.53 ± 6.00 (white varieties of bajakah).…”

Section: Discussionsupporting

confidence: 90%

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Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage

Santos,

Costa,

Bessa

et al. 2023

Explor Foods Foodomics

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Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 µg/mL of EAE (11.29%) in comparison to 15.0 µg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 µg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. Conclusions: The extraction residue from neem leaf after hexane extraction is a source important of cytotoxic and genotoxic molecules against TK6 cells, the results also can suggest that the toxic effects in TK6 cells can be provided most likely due to the presence of high content of TPC from neem extracts.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage

Santos,

Costa,

Bessa

et al. 2023

Explor Foods Foodomics

View full text Add to dashboard Cite

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“…Silva et al [45] indicated higher values for the effective diffusion coefficient with the increase in the amount of solvent in the extraction medium. Shewale et al [46] reported that increasing the sample to ethanol ratio from 1:10 to 1:20 favors the extraction of phenolic compounds, which was evidenced by the extraction kinetics and calculation of the process parameters (rate constant and capacity constant).…”

Section: Effect Of Sample To Solvent Ratiomentioning

confidence: 99%

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

de Mello,

Hoscheid,

Raspe

et al. 2024

AppliedChem

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This work evaluated the green extraction of oleoresin from pink pepper fruits (ORPPF) using ultrasound-assisted extraction (UAE) and ethanol as a solvent. The effects of temperature, ultrasound power intensity, sample to solvent ratio and time on the global extraction yield (YGE) and phenolic compounds yield (YPC) were evaluated. The oleoresin samples were characterized and its antimicrobial activity determined, and the obtained results were compared to conventional extraction in Soxhlet. From the results it was found that the application of the highest levels of the independent variables favored the extraction process. The maximum values of YGE and YPC were 28.60 wt% and 6.37 mg GAE per g fruit, respectively, obtained at 60 °C, 100% of ultrasound power (165 W), 1:20 g mL−1 (sample:solvent) and 45 min. Under maximized conditions, the ORPPF obtained by UAE showed a content of phenolic compounds and antioxidant activity inferior to soxhlet–ethanol extraction. However, the time and solvent consumption were reduced. Oleic and linoleic acids predominated in the fatty acid composition of ORPPF, in addition to sesquiterpenes and gallic and syringic acids. The ORPPF presented weak antibacterial activity, with minimum inhibitory concentration ranging from 31.25 to 125 mg mL−1.

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Numerical modeling and evaluation of solid-liquid extraction with pressurized hot water extraction applied to Robinia Pseudoacacia wood

Sangaré,

Caré,

Buron

et al. 2024

Chemical Engineering and Processing - Process Intensification

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Intensification of total phenolic compounds extraction from Azadirachta indica (Neem) leaves by ultrasound

Cited by 6 publications

References 45 publications

Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage

Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

Numerical modeling and evaluation of solid-liquid extraction with pressurized hot water extraction applied to Robinia Pseudoacacia wood

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