Intelligent Photosensitive Mesenchymal Stem Cells and Cell-Derived Microvesicles for Photothermal Therapy of Prostate Cancer

Huang, Liqun; Xu, Chang; Xu, Peng; Yu, Qiang; Chen, Mengwei; Feng, Qishuai; Pan, Jing; Cheng, Qian; Liang, Feng; Wen, Xiaofei; Wang, Ying; Shi, Yufang; Cheng, Yu

doi:10.7150/ntno.28450

Cited by 34 publications

(33 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar approach to the one described by the Aboody group was performed on modified MSCs by loading them with gold nanoparticles (nanostarts) to improve phototherapy. Although studied in a heterotopic model of prostate cancer, the results support the use of MSC to maximize clinically relevant gold nanoparticles’ optical–electronic properties by increasing nanoparticle intratumor distribution ( Huang L. et al, 2019 ).…”

Section: Applications Of Nanoparticles In Stem Cell Glioma Therapymentioning

confidence: 71%

Nanoparticles for Stem Cell Therapy Bioengineering in Glioma

Ruiz‐Garcia

Alvarado-Estrada

Krishnan

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Gliomas are a dismal disease associated with poor survival and high morbidity. Current standard treatments have reached a therapeutic plateau even after combining maximal safe resection, radiation, and chemotherapy. In this setting, stem cells (SCs) have risen as a promising therapeutic armamentarium, given their intrinsic tumor homing as well as their natural or bioengineered antitumor properties. The interplay between stem cells and other therapeutic approaches such as nanoparticles holds the potential to synergize the advantages from the combined therapeutic strategies. Nanoparticles represent a broad spectrum of synthetic and natural biomaterials that have been proven effective in expanding diagnostic and therapeutic efforts, either used alone or in combination with immune, genetic, or cellular therapies. Stem cells have been bioengineered using these biomaterials to enhance their natural properties as well as to act as their vehicle when anticancer nanoparticles need to be delivered into the tumor microenvironment in a very precise manner. Here, we describe the recent developments of this new paradigm in the treatment of malignant gliomas.

show abstract

Section: Applications Of Nanoparticles In Stem Cell Glioma Therapymentioning

confidence: 71%

Nanoparticles for Stem Cell Therapy Bioengineering in Glioma

Ruiz‐Garcia

Alvarado-Estrada

Krishnan

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In particular, several authors have shown that MSCs easily internalize different types of nanoparticles [27] and can reach the tumor, showing limited or no toxicity effect of NPs to MSCs [58,59]. Additionally, other authors have investigated whether MSCs can transport nanoparticles for therapeutic purposes [22,60,61].…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stromal cells mediated delivery of photoactive nanoparticles inhibits osteosarcoma growth in vitro and in a murine in vivo ectopic model

Lenna

Bellotti

Duchi

et al. 2020

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Background: Osteosarcoma (OS) is an aggressive malignant neoplasm that still suffers from poor prognosis in the case of distal metastases or occurrence of multi-drug resistance. It is therefore crucial to find novel therapeutic options able to go beyond these limitations and improve patients' survival. The objective of this study is to exploit the intrinsic properties of mesenchymal stromal cells (MSCs) to migrate and infiltrate the tumor stroma to specifically deliver therapeutic agents directly to cancer cells. In particular, we aimed to test the efficacy of the photoactivation of MSCs loaded with nanoparticles in vitro and in a murine in vivo ectopic osteosarcoma model. Methods: AlPcS 4 @FNPs were produced by adding tetra-sulfonated aluminum phthalocyanine (AlPcS 4) to an aqueous solution of positively charged poly-methyl methacrylate core-shell fluorescent nanoparticles (FNPs). The photodynamic therapy (PDT) effect is achieved by activation of the photosensitizer AlPcS 4 in the near-infrared light with an LED source. Human MSCs were isolated from the bone marrow of five donors to account for inter-patients variability and used in this study after being evaluated for their clonogenicity, multipotency and immunophenotypic profile. MSC lines were then tested for the ability to internalize and retain the nanoparticles, along with their migratory properties in vitro. Photoactivation effect was evaluated both in a monolayer (2D) co-culture of AlPcS 4 @FNPs loaded MSCs with human OS cells (SaOS-2) and in tridimensional (3D) multicellular spheroids (AlPcS 4 @FNPs loaded MSCs with human OS cells, MG-63). Cell death was assessed by AnnexinV/PI and Live&Dead CalceinAM/EthD staining in 2D, while in the 3D co-culture, the cell killing effect was measured through ATP content, CalceinAM/EthD staining and TEM imaging. We also evaluated the effectiveness of AlPcS 4 @FNPs loaded MSCs as delivery systems and the ability of the photodynamic treatment to kill cancer cells in a subcutaneous mouse model of OS by bioluminescence imaging (BLI) and histology. Results: MSCs internalized AlPcS 4 @FNPs without losing or altering their motility and viability in vitro. Photoactivation of AlPcS 4 @FNPs loaded MSCs induced high level of OS cells death in the 2D co-culture. Similarly, in the 3D co-culture (MSCs:OS ratios 1:1 or 1:3), a substantial decrease of both MSCs and OS cells viability was observed. Notably, when increasing the MSCs:OS ratio to 1:7, photoactivation still caused more than 40% cells death. When tested in an in vivo ectopic OS model, AlPcS4@FNPs loaded MSCs were able to decrease OS growth by 68% after two cycles of photoactivation.

show abstract

“…Another valuable tool for targeted PTT of prostate cancer is so-called mesenchymal stem cell-derived gold star nanoparticles produced directly in the cells and secreted in a form of microvesicles based upon a light irradiation stimulus. The main advantage of such nanoparticles is the fact that they exhibit up to 90% efficacy of the photothermal transformation [62,63].…”

Section: Photothermal Therapymentioning

confidence: 99%

Quo Vadis Advanced Prostate Cancer Therapy? Novel Treatment Perspectives and Possible Future Directions

et al. 2021

View full text Add to dashboard Cite

Prostate cancer is a very common disease, which is, unfortunately, often the cause of many male deaths. This is underlined by the fact that the early stages of prostate cancer are often asymptomatic. Therefore, the disease is usually detected and diagnosed at late advanced or even metastasized stages, which are already difficult to treat. Hence, it is important to pursue research and development not only in terms of novel diagnostic methods but also of therapeutic ones, as well as to increase the effectiveness of the treatment by combinational medicinal approach. Therefore, in this review article, we focus on recent approaches and novel potential tools for the treatment of advanced prostate cancer; these include not only androgen deprivation therapy, antiandrogen therapy, photodynamic therapy, photothermal therapy, immunotherapy, multimodal therapy, but also poly(ADP-ribose) polymerase, Akt and cyclin-dependent kinase inhibitors.

show abstract

Intelligent Photosensitive Mesenchymal Stem Cells and Cell-Derived Microvesicles for Photothermal Therapy of Prostate Cancer

Cited by 34 publications

References 65 publications

Nanoparticles for Stem Cell Therapy Bioengineering in Glioma

Nanoparticles for Stem Cell Therapy Bioengineering in Glioma

Mesenchymal stromal cells mediated delivery of photoactive nanoparticles inhibits osteosarcoma growth in vitro and in a murine in vivo ectopic model

Quo Vadis Advanced Prostate Cancer Therapy? Novel Treatment Perspectives and Possible Future Directions

Contact Info

Product

Resources

About