Intellectual property and assisted reproductive technology

Cyranoski, David; Contreras, Jorge L.; Carrington, Victoria T

doi:10.1038/s41587-022-01592-9

Cited by 15 publications

(4 citation statements)

References 40 publications

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“…Recently, follicles have been generated entirely from mouse PSCs without the use of donor tissues in vitro (Yoshino et al ., 2021). Thus, wider application for mechanistic studies as well as new avenues in assisted reproduction are anticipated (Cyranoski et al, 2023; Saitou and Hayashi, 2021). Similar technology is already considered for obtaining human germ cells (Hwang et al, 2020; Irie et al, 2015; Sasaki et al ., 2015; Yamashiro et al, 2018), and PGCLCs from endangered species to rescue animals from extinction (Hayashi et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive comparison of female germ cell developmentin vitroandin vivoidentifies epigenetic gene regulation crucial for oocyte development and embryonic competence

Aizawa

Ozonov

Kawamura

et al. 2023

Preprint

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Germ cells are the origin of new individuals. Hence, specifying germ cell identity is crucial for reproduction. The recent establishment of in vitro culture systems for generating oocytes from mouse pluripotent stem cells provides a basis for progress in studies of oogenesis and reproductive technology. However, currently the developmental competence of in vitro generated oocytes is low compared to in vivo grown oocytes. The causes underlying poor oocyte quality remain to be determined. By reconstituting germ cell development in culture from different developmental starting points within gametogenesis, we show that the differentiation of primordial germ cells (PGCs) and primordial germ cell-like cells (PGCLCs) to growing oocytes (GROs), as well as the subsequent growth of follicles are critical culture steps for specifying competence of fully-grown oocytes (FGOs) for preimplantation development. A systematic comparison of transcriptomes of single oocytes having undergone different in vitro culture trajectories identifies genes normally upregulated during oocyte growth to be susceptible for mis-regulation during in vitro oogenesis. Many of such genes have been described as targets of Polycomb repressive complexes (PRCs). Deregulation of Polycomb repression therefore likely perturbs the accumulation of cytoplasmic factors and/or setting of chromatin states in FGOs that are required for embryonic development after fertilization. Conversely, in vitro derived oocytes often displayed failure of zygotic genome activation (ZGA) and abnormal acquisition of 5-hydroxymethylcytosine (5hmC) on maternal chromosomes after activation. In addition, subcellular delocalization of pyruvate dehydrogenase (PDH) and of STELLA were observed suggesting new molecular markers for defective oocyte development. Our study identifies epigenetic regulation at an early stage of oogenesis as crucial for developmental competence and suggests specific in vitro culture steps as targets for improving oocyte quality.

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Section: Discussionmentioning

confidence: 99%

Comprehensive comparison of female germ cell developmentin vitroandin vivoidentifies epigenetic gene regulation crucial for oocyte development and embryonic competence

Aizawa

Ozonov

Kawamura

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Also, functional oocytes were generated from PSCs of male mice by the in vitro culture (Murakami et al, 2023). Thus, wider application for mechanistic studies as well as new avenues in assisted reproduction is anticipated (Saitou & Hayashi, 2021;Cyranoski et al, 2023). Similar technology is already considered for obtaining human germ cells (Irie et al, 2015;Sasaki et al, 2015;Yamashiro et al, 2018;Hwang et al, 2020), and PGCLCs from endangered species to rescue animals from extinction (Hayashi et al, 2022).…”

Section: Transcriptome Comparison and Modeling Identifies Differentia...mentioning

confidence: 99%

Epigenetic regulation limits competence of pluripotent stem cell‐derived oocytes

Aizawa,

Ozonov,

Kawamura

et al. 2023

The EMBO Journal

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Recent studies have reported the differentiation of pluripotent cells into oocytes in vitro. However, the developmental competence of in vitro‐generated oocytes remains low. Here, we perform a comprehensive comparison of mouse germ cell development in vitro over all culture steps versus in vivo with the goal to understand mechanisms underlying poor oocyte quality. We show that the in vitro differentiation of primordial germ cells to growing oocytes and subsequent follicle growth is critical for competence for preimplantation development. Systematic transcriptome analysis of single oocytes that were subjected to different culture steps identifies genes that are normally upregulated during oocyte growth to be susceptible for misregulation during in vitro oogenesis. Many misregulated genes are Polycomb targets. Deregulation of Polycomb repression is therefore a key cause and the earliest defect known in in vitro oocyte differentiation. Conversely, structurally normal in vitro‐derived oocytes fail at zygotic genome activation and show abnormal acquisition of 5‐hydroxymethylcytosine on maternal chromosomes. Our data identify epigenetic regulation at an early stage of oogenesis limiting developmental competence and suggest opportunities for future improvements.

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“…In assisted reproductive technology (ART), in vitro fertilization (IVF) is usually considered the most productive approach. 12 in vitro fertilization (IVF) is widely recognized as a very effective treatment for infertility, yet it sometimes requires numerous rounds of egg retrieval and embryo transfer. 13 This treatment has shown efficacy in addressing unexplained infertility and conditions such as blocked or damaged fallopian tubes, hereditary abnormalities, uterine fibroids in women, ovulation difficulties, early ovarian failure, and cases where ovaries have been surgically removed.…”

Section: Introductionmentioning

confidence: 99%

The assessment of educational and supportive care to the infertile females undergoes In Vitro Fertilization procedure by clinical pharmacist: a randomized clinical trial

Abbas Ibrahim,

Abdulhadi Rashid

2023

F1000Res

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Background Infertility affects approximately 10-15% of married individuals, and may trigger various emotional reactions. Females undergoing in vitro fertilization (IVF) are more susceptible to experiencing depression. This study aims to assess the influence of pharmacist counseling on quality of life, depression scores, and pregnancy rates among infertile females undergoing IVF treatment. Method The research examined women who visited the infertility clinic at Kamal Al-Samaraie Hospital in Baghdad, Iraq to achieve pregnancy. These individuals were separated into a control group and an intervention group. The intervention group received an educational program consisting of five visits, while the control group got a conventional hospital procedure. The present research evaluated the quality of life-related to fertility using the FertiQoL (Fertility Quality of Life) questionnaire. It examined the participants’ depression levels using the CES-D (Center for Epidemiologic Studies Depression) scale. These assessments were conducted at the commencement and conclusion of the in vitro fertilization (IVF) cycle. Results The present research included 150 women diagnosed with infertility, whose average age was 30.32 ± 5.64 years. The participants had a mean body mass index (BMI) of 27.12 ± 3.57. No statistically significant difference was seen in the FertiQoL evaluation scores between the control and intervention groups at the beginning of the study. The FertiQoL, assessed at the end of the trial, exhibited a statistically significant increase in the interventional group, with a P-value of 0.025. The prevalence of Depression risk patients decreased from 83% to 69%, and the interventional group exhibited a substantially greater proportion of favorable pregnancy outcomes (48%) compared to the control group (29.3%). Conclusion Infertile women who undergo IVF operations have higher rates of depression. Depression may be reduced, and quality of life improved with education and supportive counseling. Pregnancy rates rise as a result of clinical pharmacist counseling. Trial registration This study was registered on Clinical Trials.gov (NCT06022640).

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Intellectual property and assisted reproductive technology

Cited by 15 publications

References 40 publications

Comprehensive comparison of female germ cell developmentin vitroandin vivoidentifies epigenetic gene regulation crucial for oocyte development and embryonic competence

Comprehensive comparison of female germ cell developmentin vitroandin vivoidentifies epigenetic gene regulation crucial for oocyte development and embryonic competence

Epigenetic regulation limits competence of pluripotent stem cell‐derived oocytes

The assessment of educational and supportive care to the infertile females undergoes In Vitro Fertilization procedure by clinical pharmacist: a randomized clinical trial

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