Integrons in Enterobacteriaceae : diversity, distribution and epidemiology

Kaushik, Megha; Kumar, Sanjay; Kapoor, Rajeev Kumar; Virdi, Jugsharan Singh; Gulati, Pooja

doi:10.1016/j.ijantimicag.2017.10.004

Cited by 85 publications

(59 citation statements)

References 150 publications

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“…Integrons are genetic elements found in bacteria, which can capture and express antimicrobial resistance gene cassettes [1]. Integrons are strongly associated with the dissemination of antimicrobial genes among different genera of bacteria [2].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. e purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods. Eighty-three E. coli ST131 isolates were used in this study. e antibiotic susceptibility test was performed by the disk diffusion method. e presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. Clonal relatedness of E. coli ST131 isolates was analyzed by PFGE. Results. Overall, 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. Class 2 and 3 integrons were not found in this study. Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Conclusions. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.

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Section: Introductionmentioning

confidence: 99%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…An integron includes a gene that codes for an integrase (Intl) and a site of specific recombination (attI) [31]. The sequences of the integrase enzymes allow integron classification in different classes (I-III) [32].…”

Section: Integrons and Cassette Systemmentioning

confidence: 99%

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

Mancilla-Becerra¹,

Lías-Macías²,

Ramírez-Jiménez³

et al. 2020

Pathogenic Bacteria

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The drug abuse known to occur during growth of animals intended for food production, because of their use as either a prophylactic or therapeutic treatment, promotes the emergence of bacterial drug resistance. It has been reported that at least 25% of the foodborne isolates show drug resistance to one or more classes of antimicrobials (FAO 2018). There are diverse mechanisms that promote drug resistance. It is known that the use of sub-therapeutic doses of antibiotics in animals intended for food production promotes mutations of some chromosomal genes such as gyrA-parC and mphA, which are responsible for quinolone and azithromycin resistance, respectively. Also, the horizontal transfer of resistance genes as groups ("cassettes") or plasmids makes the spread of resistance to different bacterial genera possible, among which there could be pathogens. The World Health Organization considers the emergence of multidrug-resistant pathogenic bacteria as a health problem, since the illnesses caused by them complicate the treatment and increase the morbidity and mortality rates. The complication in the illness treatment caused by a multidrug-resistant pathogen causes economic losses to patients for the payment of long stays in hospitals and also causes economic losses to companies due to the absenteeism of their workers.

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“…Интегроны -рекомбинационная система, способная захватывать гены в форме кассет. Хотя интегроны не являются мобильными элементами сами по себе, их связь с транспозонами, IS и конъюгативными плазмидами делает их основными векторами для распространения генов устойчивости к антибиотикам внутри и между различными видами бактерий [65]. Структура интегрона изображена на рис.…”

Section: элементы осуществляющие горизонтальный перенос геновunclassified

Mechanisms of bacterial multiresistance to antibiotics

Zemlyanko

Михайловна²,

Rogoza

et al. 2018

Ecological genetics

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Multiple drug resistance (MDR) to widening range of antibiotics emerging in increasing variety of pathogenic bacteria is a serious threat to the health of mankind nowadays. This is partially due to an uncontrolled usage of antibiotics not only in clinical practice, but also in various branches of agriculture. MDR is affected by two mechanisms: (1) accumulation of resistance genes as a result of intensive selection caused by antibiotics, and (2) active horizontal transfer of resistance genes. To unveil the reasons of bacterial multiresistance to antibiotics, it is necessary to understand the mechanisms of antibiotics action as well as the ways how either resistance to certain antibiotics emerge or resistance genes accumulate and transfer among bacterial strains. Current review is devoted to all these problems.

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Integrons in Enterobacteriaceae : diversity, distribution and epidemiology

Cited by 85 publications

References 150 publications

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Multidrug-Resistant Bacterial Foodborne Pathogens: Impact on Human Health and Economy

Mechanisms of bacterial multiresistance to antibiotics

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