Integrons and β-lactamases—a novel perspective on resistance

Weldhagen, Gerhard F.

doi:10.1016/j.ijantimicag.2004.03.007

Cited by 83 publications

(39 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recent studies have indicated that integrons play a particularly important role in the spread of multidrug resistance in Gram-negative bacteria due to their ability to capture and exchange resistance genes via site-specific recombination (Ploy et al 2000;Weldhagen 2004). The detection and characterization of integrons and gene cassettes may be critical in understanding the resistance dissemination pathways and in evaluating the potential of an environment to act as an antibiotic resistance reservoir (Henriques et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

Wang

Dang

Ding

2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Environmental microbiology investigation was performed to determine the molecular diversity of b-lactamase genes among ampicillin-resistant bacteria from Jiaozhou Bay. b-lactamase genes were detected in 93.8% of the bacterial isolates identified as Enterobacteriaceae. The most frequently detected gene was bla TEM , followed by bla SHV , bla OAX-1 , bla MOX and bla CMY . Most of the isolates (68.8%) were positive for the intI1 integrase gene, and two isolates were also found for the intI2 gene. The dfr and aadA gene cassettes were predominant. Anthropogenic contamination from onshore sewage processing plants might contribute predominantly to the b-lactamase gene reservoir in the studied coastal waters. Environmental antibiotic-resistant bacteria and resistance genes may serve as bioindicators of coastal environmental quality or biotracers of the potential contamination sources. This is the first report of the prevalence and characterization of blactamase genes and integrons in coastal Enterobacteriaceae from China.

show abstract

Section: Introductionmentioning

confidence: 99%

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

Wang

Dang

Ding

2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Previous research has shown that chromosomally acquired resistance to streptomycin is frequently due to mutation or other molecular changes in the genes encoding ribosomal protein S12 (rpsL) and 16S rRNA (rrs) (Wu et al 2006). The strA-strB genes (Sundin 2000(Sundin , 2002 and aadA gene (Li and Nikaido 2004;Poole 2004;Weldhagen 2004) are also involved in streptomycin resistance. In the current research, seven streptomycin-resistant mutants were obtained by induction in the laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…syringae (Sundin and Bender 1993), and Salmonella typhimurium (Madsen et al 2000). The third mechanism involves the aadA gene, which had also been found to be associated with streptomycin resistance in many clinical bacteria (Li and Nikaido 2004;Poole 2004;Weldhagen 2004) and S. typhimurium (Madsen et al 2000).…”

Section: Introductionmentioning

confidence: 99%

A molecular mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzicola

Zhang

Zhu

et al. 2011

Phytoparasitica

View full text Add to dashboard Cite

Xanthomonas oryzae pv. oryzicola, the causal agent of rice leaf streak disease, was found to be sensitive to streptomycin (an aminocyclitol glycoside antibiotic), by inhibition of protein synthesis resulting from interference with translational proofreading. This study aimed to determine the molecular resistance mechanism of X. oryzae pv. oryzicola to streptomycin. Seven streptomycin-resistant mutants were obtained by UV induction or streptomycin selection. These mutants can grow at 100 μg ml −1 of streptomycin while the wild-type strain (RS105) cannot grow at 5 μg ml −1 . Sequencing indicated that the rpsL gene encoding ribosomal protein S12 has 375 bp encoding 125 amino acid residues. In all resistant strains, a mutation in which AAG was substituted for AGG (Lys→Arg) occurred either at codon 43 or 88. Two plasmids, pUFRRS and pUFRRX, were constructed by ligating the rpsL gene into the cosmid pUFR034. The plasmids pUFRRS and pUFRRX containing the Lys→Arg mutation of the rpsL gene conferred streptomycin resistance to the sensitive wild-type strain by electroporation. Both transformants, RS1 and RS2, could grow in the medium containing 50 μg ml −1 of streptomycin. A mutation at codon 43 or 88 in rpsL can result in resistance of Xanthomonas oryzae pv. oryzicola to streptomycin.

show abstract

“…Chromosomally acquired resistance to streptomycin is often due to mutations or other molecular changes in the genes encoding ribosomal protein S12 (rpsL) and 16S rRNA (rrs) (Wu et al, 2006). The strA-strB genes (Sundin et al, 2000(Sundin et al, , 2002 and aadA gene (Li et al, 2004;Poole et al, 2004;Weldhagen et al, 2004) are also involved in streptomycin resistance. In our previous research, laboratory-induced streptomycin resistance in X. oryzae pv.…”

Section: Introductionmentioning

confidence: 99%

Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP

Zhang¹,

Yang²,

Zhou³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP ABSTRACT. The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycinresistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of the rpsL gene in X. oryzae pv. oryzicola and X. oryzae pv. oryzae. The 304-bp PCR product from the rpsL gene was digested by MboII to form two fragments (201 and 103 bp) if there was a mutation at codon 43, or three fragments (146, 103, and 55 bp) if there was no mutation. Compared with the results from nucleotide sequencing, the PCR-RFLP method was accurate in detecting the point mutation at codon 43 of the rpsL gene in streptomycin-resistant strains of X. oryzae pv. oryzicola and X. oryzae pv. oryzae. These results indicate that the PCR-RFLP is a simple, rapid and reliable method for detecting the point mutation at codon 43 of the rpsL gene.

show abstract

Integrons and β-lactamases—a novel perspective on resistance

Cited by 83 publications

References 59 publications

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

A molecular mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzicola

Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP

Contact Info

Product

Resources

About