Integrity Profiling of High Throughput Screening Hits Using LC-MS and Related Techniques

Abstract

Integrity profiling of HTS hits is valuable for verification of the hit identity and purity. This provides early discovery researchers with more confident SAR theories. Methodology for integrity profiling of HTS hits must be high throughput, consume little material, and selectively provide structure-based data. Analytical techniques that can be utilized for integrity profiling methods are reviewed for their appropriateness in sample preparation, component separation, detection, purity quantitation, identity confirmation, and follow-up.

Epigenetic assays for chemical biology and drug discovery

“…This has been a longstanding technique and used as a secondary assay due to its relatively low throughput, or for screening modest libraries of compounds [98, 99]. It is a label-free approach as it relies upon the separation and subsequent quantification of typically a substrate and product that has undergone modification that the mass spectrometer is capable of detecting [100].…”