Integrity matters: Linking nuclear architecture to lifespan

Wilson, Katherine L.

doi:10.1073/pnas.0509224102

Cited by 13 publications

(12 citation statements)

References 21 publications

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“…Mutations in the genes encoding lamin A and its binding partners have been associated with a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, limb girdle muscular dystrophy, Dunnigan-type familial partial lipodystrophy and Hutchinson-Gilford progeria syndrome (Bonne et al, 1999;Cao and Hegele, 2000;Eriksson et al, 2003;Fatkin et al, 1999;Muchir et al, 2000). Lamin A/C deficiency and other kinds of mutation may considerably alter the nuclear lamina, jeopardizing the structural integrity of the nucleus (Fidzianska et al, 1998;Hutchison et al, 2001;Lammerding et al, 2004Lammerding et al, , 2005Worman and Courvalin, 2004;Wilson, 2005).…”

Section: Discussionmentioning

confidence: 99%

Mechanics and deformation of the nucleus in micropipette aspiration experiment

Vaziri

Mofrad

2007

Journal of Biomechanics

128

113

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Section: Discussionmentioning

confidence: 99%

Mechanics and deformation of the nucleus in micropipette aspiration experiment

Vaziri

Mofrad

2007

Journal of Biomechanics

128

113

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“…It has also been suggested that premature aging processes could be result of alteration in the structural and functional properties of nuclear lamins and their binding proteins. Wilson [2005] reported that vertebrate Lamins and lamin associated proteins are involved in gene regulation, TGF-b signaling, DNA replication, mRNA transcription and also have fundamental roles in chromatin organization, nuclear architecture and nuclear assembly.…”

Section: Discussionmentioning

confidence: 99%

“…Data from studies with [Wilson et al, 2001[Wilson et al, , 2005 C. elegance, fruitful, xenopus eggs, and cultured mammalian cells suggest that gene expression, cell signaling, chromatin organization are directly or indirectly depend on the nuclear lamina organization.…”

Section: Discussionmentioning

confidence: 99%

“…It has been proposed that [Wilson, 2005] nuclear lamina filaments provide Scaffolds for the assembly, and sequestration of many proteins in the nucleus that regulate gene expression. We believe that the two most abundant nuclear matrix protein of lens epithelial cells, Lamin C and HSF-1 are probably associated with each other for their activities.…”

Section: Discussionmentioning

confidence: 99%

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Lamins of ocular lens epithelial cells

Bagchi

Petrov

Maisel

2006

J of Cellular Biochemistry

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Experiments were performed to characterize a prominent nuclear matrix (NM) protein isolated from tissue cultured mouse lens epithelial cells. This NM protein was separated by SDS-PAGE and the stained gel band was analyzed by mass spectroscopy. Blast analysis of the amino acid sequence derived by mass spectroscopy revealed the presence of Lamin C in the NM of the mouse lens epithelial cells. We also examined nuclear proteins of adult and fetal human lenses. Data collected from these experiments showed the presence of Lamin C in both adult and fetal lens cells. However fetal lens cells only show Lamin C dimers, whereas adult human lens contained dimers, monomers and degraded Lamin C. Early and late passaged tissue cultured mouse lens epithelial cells also contained Lamin C in the nucleus with a preponderance of the dimer in the early passaged cells. The biological significance of the presence of dimers in human fetal lens cells and early passaged mouse lens cells is not known. However, it could suggest an enhanced docking capability of Lamin C dimers for other physiologically important nuclear proteins.

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“…Initiation and progression of many diseases are associated with alterations in the structure and function of subcellular and subnuclear components which in turn modify the function of the cell [Cornhill and Roach 1974;Sinzinger et al 1980;Athanasiou and Shieh 2002;Trickey et al 2000; [Lammerding et al 2004;Wilson 2005;Mattout et al 2006]. As another example, various cancer cells have altered nuclear structure and irregularity in shapes and spatial organization of subnuclear structures [Zink et al 2004].…”

Section: Implications In Understanding the State Of Human Health And mentioning

confidence: 99%

Continuum-based computational models for cell and nuclear mechanics

Vaziri

Gopinath

Deshpande

2007

JOMMS

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Deciphering the relationship between cellular processes and the structure of living cells is a key step toward understanding and predicting cell functions with direct implications for understanding human health and disease. The active nature of these cellular processes, which span several decades of spatial and temporal scales, pose significant challenges to unraveling this complex structure-function paradigm.Complementing novel experimental techniques with robust computational approaches capable of modeling mechanical response at varying scales provides new avenues to resolving this paradigm. We provide an overview of continuum-based computational approaches used in studying and interpreting responses of individual cells and nuclei, we outline techniques used for measuring the mechanical characteristics of living cells, and we discuss some of the key insights provided by these approaches.

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Integrity matters: Linking nuclear architecture to lifespan

Cited by 13 publications

References 21 publications

Mechanics and deformation of the nucleus in micropipette aspiration experiment

Mechanics and deformation of the nucleus in micropipette aspiration experiment

Lamins of ocular lens epithelial cells

Continuum-based computational models for cell and nuclear mechanics

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