Integrin trafficking in cells and tissues

Moreno-Layseca, Paulina; Icha, Jaroslav; Hamidi, Hellyeh; Ivaska, Johanna

doi:10.1038/s41556-018-0223-z

Cited by 296 publications

(301 citation statements)

References 148 publications

(184 reference statements)

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“…Several studies have shown that nociceptive sensory terminals share a close relationship with specific extracellular structures, most notably epidermal cells and extracellular matrix (ECM), yet whether paclitaxel impact this relationship is unknown. Integrins are a key mediator of the interaction between cells and the ECM, and impact neuronal maintenance in both vertebrate and invertebrate systems (Han et al, 2012;Kim et al, 2012;Moreno-Layseca et al, 2019;Nieuwenhuis et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Conceivably, these changes could include reduction in de novo integrin synthesis, reduced integrin recycling, and/or increased integrin degradation. Prior studies in other systems indicate that integrin levels are maintained by continuous recycling via tight regulation of the endosomal pathway rather than degradation and de novo synthesis (Moreno-Layseca et al, 2019). We therefore investigated potential changes caused by paclitaxel in endosomal and lysosomal pathways in nociceptive neurons in Drosophila.…”

Section: Paclitaxel Disrupts Endosomal-lysosomal Pathways and Changesmentioning

confidence: 99%

“…identified paclitaxel-mediated morphological changes in nociceptive neurons, and report quantitative changes in branch pattern arising from paclitaxel exposure. We report that overexpressing integrins, cell surface receptors critical during development and maintenance of non-neuronal cells and neurons(Hynes, 1992;Moreno-Layseca et al, 2019), protects nociceptive neuron morphology and function in Drosophila, and reduces axon degeneration in adult DRG neurons in vitro. We combined live imaging, high resolution and superresolution microscopy to identify the intracellular mechanisms that underlie integrin-mediated protection.…”

mentioning

confidence: 99%

“…Arjonen et al, 2012;Moreno-Layseca et al, 2019;Roberts et al, 2001). Perturbation of Rab4 motility and availability would likely lead to reduced integrin turnover.…”

mentioning

confidence: 99%

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Integrins protect nociceptive neurons in models of paclitaxel-mediated peripheral sensory neuropathy

et al. 2019

Preprint

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Section: Resultsmentioning

confidence: 99%

Section: Paclitaxel Disrupts Endosomal-lysosomal Pathways and Changesmentioning

confidence: 99%

mentioning

confidence: 99%

“…Arjonen et al, 2012;Moreno-Layseca et al, 2019;Roberts et al, 2001). Perturbation of Rab4 motility and availability would likely lead to reduced integrin turnover.…”

mentioning

confidence: 99%

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Integrins protect nociceptive neurons in models of paclitaxel-mediated peripheral sensory neuropathy

et al. 2019

Preprint

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“…AP-2 is well known for its role in facilitating clathrin-mediated endocytosis of integrins (De Franceschi et al, 2016;Moreno-Layseca et al, 2019). Thus we wondered, if AP-1B would colocalize with AP-2.…”

Section: Ap-1b Does Not Colocalize With Ap-2mentioning

confidence: 96%

AP-1B facilitates endocytosis during cell migration

Kell¹,

Ang²,

Pigati³

et al. 2019

Preprint

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The epithelial cell-specific clathrin adaptor AP-1B has a well-established role in polarized sorting of cargos to the basolateral membrane. Here we demonstrate a novel function for AP-1B during collective cell migration of epithelial sheets. We show that AP-1B colocalized with b1 integrin in focal adhesions during cell migration using confocal microscopy and total internal reflection fluorescence (TIRF) microscopy on fixed specimens. Further, AP-1B labeling in cell protrusion was distinct from labeling for the canonical endocytic adaptor complex AP-2. Using stochastic optical reconstruction microscopy (STORM) and live TIRF imaging we identified numerous AP-1B-coated structures at or close to the plasma membrane in cell protrusions.Importantly, immuno-electron microscopy (EM) showed AP-1B in clathrin-coated pits and budding vesicles at the plasma membrane during cell migration. Our data therefore established a novel function for AP-1B in endocytosis. We further show that b1 integrin was dependent on AP-1B and its co-adaptor, autosomal recessive hypercholesterolemia protein (ARH), for sorting to the basolateral membrane. Notably, we found that expression of AP-1B (and ARH) slowed epithelial-cell migration, and qRT-PCR analysis of human epithelial-derived cell lines revealed a loss of AP-1B expression in highly metastatic cancer cells indicating that AP-1B-facilitated endocytosis during cell migration might be an anti-cancer mechanism.

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Spatiotemporal Regulation of the Bone Immune Microenvironment via Dam‐Like Biphasic Bionic Periosteum for Bone Regeneration

Tang

et al. 2022

Adv Healthcare Materials

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The bone immune microenvironment (BIM) regulates bone regeneration and affects the prognosis of fractures. However, there is currently no effective strategy that can precisely modulate macrophage polarization to improve BIM for bone regeneration. Herein, a hybridized biphasic bionic periosteum, inspired by the BIM and functional structure of the natural periosteum, is presented. The gel phase is composed of genipin-crosslinked carboxymethyl chitosan and collagen self-assembled hybrid hydrogels, which act as the "dam" to intercept IL-4 released during the initial burst from the bionic periosteum fiber phase, thus maintaining the moderate inflammatory response of M1 macrophages for mesenchymal stem cell recruitment and vascular sprouting at the acute fracture. With the degradation of the gel phase, released IL-4 cooperates with collagen to promote the polarization towards M2 macrophages, which reconfigure the local microenvironment by secreting PDGF-BB and BMP-2 to improve vascular maturation and osteogenesis twofold. In rat cranial defect models, the controlled regulation of the BIM is validated with the temporal transition of the inflammatory/anti-inflammatory process to achieve faster and better bone defect repair. This strategy provides a drug delivery system that constructs a coordinated BIM, so as to break through the predicament of the contradiction between immune response and bone tissue regeneration.

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Integrin trafficking in cells and tissues

Cited by 296 publications

References 148 publications

Integrins protect nociceptive neurons in models of paclitaxel-mediated peripheral sensory neuropathy

Integrins protect nociceptive neurons in models of paclitaxel-mediated peripheral sensory neuropathy

AP-1B facilitates endocytosis during cell migration

Spatiotemporal Regulation of the Bone Immune Microenvironment via Dam‐Like Biphasic Bionic Periosteum for Bone Regeneration

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