Integrin-Dependent Interaction of Human Vascular Endothelial Cells on Biomimetic Peptide Surfactant Polymers

Murugesan, Gurunathan; Ruegsegger, Mark; Kligman, Faina; Marchant, Roger; Kottke‐Marchant, Kandice

doi:10.1080/15419060214148

Cited by 21 publications

(14 citation statements)

References 65 publications

(98 reference statements)

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“…In their resting state, arterial endothelial cells express predominantly integrins ␣ 5 ␤ 1 , ␣ V ␤ 3 , and ␣ 2 ␤ 1 , which are receptors for the ECM proteins fibronectin, vitronectin, and collagen, respectively. 6 In the present study, all PDMS substrates, smooth and grooved alike, were uniformly covered with the same areal density of fibronectin. Because of this, the ability of fibronectin to promote integrin-mediated adhesion of endothelial cells was similar for all substrates.…”

Section: Discussionmentioning

confidence: 99%

“…The flow rates of CF 4 , SF 6 , and O 2 , the pressure, and the etch time used to achieve channel depths from 200 nm to 5 m are given in Table I. After RIE, the photomask was removed in a solution containing 155 g ceric ammonium nitrate, 60 mL nitric acid, and 985 mL deionized water.…”

Section: Microfabrication Of Silicon Moldmentioning

confidence: 99%

“…1 For the case of a polymeric template, successful adhesion of endothelial cells to a typically inert, unmodified polymer depends on the chemical constitution of the surface. Surface modifications, such as the adsorption of extracellular matrix (ECM) proteins [2][3][4] and the covalent attachment of specific RGD peptide sequences, 1,[5][6][7] have been found to promote endothelial cell adhesion onto a variety of synthetic materials. Relative to studies involving surface chemistry, systematic studies of surface topography have only recently received significant attention.…”

Section: Introductionmentioning

confidence: 99%

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Topographic guidance of endothelial cells on silicone surfaces with micro‐ to nanogrooves: Orientation of actin filaments and focal adhesions

Uttayarat

Toworfe

Dietrich

et al. 2005

J Biomedical Materials Res

185

174

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To mimic the uniformly elongated endothelium in natural linear vessels, bovine aortic endothelial cells (BAECs) are cultured on micro- to nanogrooved, model poly(dimethylsiloxane) (PDMS) substrates preadsorbed with about 300 ng/cm(2) of fibronectin. BAEC alignment, elongation, and projected area were investigated for channel depths of 200 nm, 500 nm, 1 microm, and 5 microm, as well as smooth surfaces. Except for the 5 microm case, the ridge and channel widths were held nearly constant about 3.5 microm. With increasing channel depth, the percentage of aligned BAECs increased by factors of 2, 2, 1.8, and 1.7 for 1, 4, 24, and 48 h. Maximum alignment, about 90%, was observed for 1 microm deep channels at 1 h. The alignment of BAECs on grooved PDMS was maintained at least until cells reached near confluence. F-actin and vinculin at focal adhesions also aligned with channel direction. Analysis of confocal microscopy images showed that focal adhesions localized at corners and along the sidewalls of 1-microm deep channels. In contrast, focal adhesions could not form on the bottom of the 5-microm deep channels. Cell proliferation was similar on grooved and smooth substrates. In summary, PDMS substrates engraved with micro- and nanochannels provide a powerful method for investigating the interplay between topography and cell/cytoskeletal alignment.

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Section: Discussionmentioning

confidence: 99%

Section: Microfabrication Of Silicon Moldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Topographic guidance of endothelial cells on silicone surfaces with micro‐ to nanogrooves: Orientation of actin filaments and focal adhesions

Uttayarat

Toworfe

Dietrich

et al. 2005

J Biomedical Materials Res

185

174

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“…Endothelial cells are known to express integrin alpha 5 and beta 1 [21], both of which interact with an arginine-glycine-aspartic acid (RGD) peptide carried by FN. The integrin-mediated cell adhesion seems to be crucial for anchorage-dependent endothelial cells for their proliferation and cell-cell network formation [22,23].…”

Section: Preparation Of Culture Substratementioning

confidence: 99%

A thin carboxymethyl cellulose culture substrate for the cellulase-induced harvesting of an endothelial cell sheet

Kato²,

Iwata³

2005

Journal of Biomaterials Science, Polymer Edition

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Engineered tissues constructed with two-dimensionally organized cells provide promising parts for reconstructing damaged tissues. Here we propose a new method for fabricating a 2D sheet made of an endothelial cell monolayer. First a culture substrate was prepared by treating the glass surface with an amine-terminated organosilicon derivative, followed by the covalent attachment of a thin carboxymethyl cellulose (CMC) layer. Fibronectin was immobilized onto the CMC-coated surface to promote cell adhesion. These surfaces were characterized step by step by means of contact angle measurement and X-ray photoelectron spectroscopy. Porcine aortic endothelial cells adhered to the culture substrate and consequently formed a confluent monolayer. When the substrate-cell composite was immersed in a cellulase solution, a cell sheet was spontaneously detached from the substrate due to enzymatic digestion of the CMC layer. The cell-cell connections were well preserved in the cell sheet, even after detachment from the substrate, most likely due to the fact that cellulase is harmless to mammalian cells. The cell sheet could be transferred to other culture dish with the aid of a hydrophilic membrane support, retaining the proliferation activity of the cells. The results obtained in this study demonstrate that cellulase treatment of the CMC layer is a rational and efficient method for obtaining a 2D cell sheet.

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“…Up to date, the approach to prevent thrombosis and to improve the biocompatibility of synthetic grafts is to introduce a functional monolayer of endothelial cells onto the graft surface [1]. This requires modifications of polymer surface such as adsorption with extracellular matrix (ECM) proteins [2][3][4] or covalent attachment with RGD peptide sequences [1,[5][6][7] to promote cell adhesion. Additional to surface chemical composition, topographic textures of the surface also guides changes in the morphology and the orientation of adherent endothelial cells [4,8].…”

Section: Introductionmentioning

confidence: 99%

Effect of Nano-to Micro-Scale Surface Topography on the Orientation of Endothelial Cells

2004

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The effect of grating textures on the alignment of cell shape and intracellular actin cytoskeleton has been investigated in bovine aortic endothelial cells (BAECs) cultured on a model cross-linked poly(dimethylsiloxane) (PDMS). Grating-textured PDMS substrates, having a variation in channel depths of 200 nm, 500 nm, 1 µm and 5 µm, were coated with fibronectin (Fn) to promote endothelial cell adhesion and cell orientation. As cells adhered to the Fn-coated surface, the underlying grating texture has shown to direct the alignment of cell shape, F-actin and focal contacts parallel to the channels. Cell alignment was observed to increase with increasing channel depths, reaching the maximum orientation where most cells aligned parallel to channels on 1-µm textured surface. Immunofluorescence studies showed that F-actin stress fibers and vinculin at focal contacts also aligned parallel to the channels. Cell proliferation was found to be independent of grating textures and the alignment of cell shape was maintained at confluence. ABSTRACTThe effect of grating textures on the alignment of cell shape and intracellular actin cytoskeleton has been investigated in bovine aortic endothelial cells (BAECs) cultured on a model cross-linked poly(dimethylsiloxane) (PDMS). Grating-textured PDMS substrates, having a variation in channel depths of 200 nm, 500 nm, 1 µm and 5 µm, were coated with fibronectin (Fn) to promote endothelial cell adhesion and cell orientation. As cells adhered to the Fn-coated surface, the underlying grating texture has shown to direct the alignment of cell shape, F-actin and focal contacts parallel to the channels. Cell alignment was observed to increase with increasing channel depths, reaching the maximum orientation where most cells aligned parallel to channels on 1-µm textured surface. Immunofluorescence studies showed that F-actin stress fibers and vinculin at focal contacts also aligned parallel to the channels. Cell proliferation was found to be independent of grating textures and the alignment of cell shape was maintained at confluence.

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Integrin-Dependent Interaction of Human Vascular Endothelial Cells on Biomimetic Peptide Surfactant Polymers

Cited by 21 publications

References 65 publications

Topographic guidance of endothelial cells on silicone surfaces with micro‐ to nanogrooves: Orientation of actin filaments and focal adhesions

Topographic guidance of endothelial cells on silicone surfaces with micro‐ to nanogrooves: Orientation of actin filaments and focal adhesions

A thin carboxymethyl cellulose culture substrate for the cellulase-induced harvesting of an endothelial cell sheet

Effect of Nano-to Micro-Scale Surface Topography on the Orientation of Endothelial Cells

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