Integrative transcriptomics and proteomics analysis of longissimus dorsi muscles of Canadian double-muscled Large White pigs

Li, Shuqin; Han, Wenpeng; Jiang, Shunyan; Zhao, Chunjiang; Wu, Changxin

doi:10.1016/j.gene.2015.11.016

Cited by 10 publications

(6 citation statements)

References 65 publications

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“…We found that the IMF of Mashen pigs was 3.73% and Large White pigs was 2.51%. Consistently, it has been reported that the IMF of European pigs is 2% to 3%, while in Chinese indigenous pigs, the IMF is around 4% or even higher (Liu et al 2016;Yang et al 2016). In addition, Mashen pigs exhibited reduced loin eye area, reduced lean meat rate, lower drip loss, lower WBSF, and lower lightness values than Large White pigs.…”

Section: Discussionsupporting

confidence: 56%

Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

Guo

Qin

Yang

et al. 2019

Italian Journal of Animal Science

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The aim of this study was to evaluate the differences in carcase traits, meat quality and muscle characteristics (mean fibre diameter and MyHCs levels of expression) between Mashen and Large White pigs. A total of 60 pigs (30 Mashen pigs and 30 Large White pigs, half male and half female in each breed) were used in this experiment. Results showed that Mashen pigs exhibited lower slaughter weight, reduced back-fat thickness, reduced loin eye area and lower lean meat ratio than Large White pigs (p < .05). Meat from Large White pigs had higher pH1, greater drip losses, higher Warner-Bratzler shear force values, higher lightness, higher yellowness, and greater mean diameter of muscle fibres. Also, meat from Large white pigs had lower cooked yield, lower redness, and reduced intramuscular fat content than meat from Mashen pigs (p < .05). The contents of 17 amino acids, total amino acids, essential amino acids and umami amino acids in the longissimus dorsi of Mashen pigs were higher than those in Large White pigs (p < .05). Large White pigs had higher monounsaturated fatty acids and lower polyunsaturated fatty acids than Mashen pigs (p < .05). Meanwhile, the expression of MyHCI was significantly higher and the expression of MyHCIIb was lower in Mashen than in Large White pigs (p < .05). The higher MyHCI and lower MyHCIIb found in muscles from Mashen pigs might partially explain meat quality differences found between the two breeds in the present study. These results provide valuable information for meat quality differences between Mashen and Large White pigs. HIGHLIGHTSCarcase traits and meat quality between Large White and Mashen pigs exhibited significant differences. The total amino acids, essential amino acids and umami amino acids in Mashen pigs were higher than those in Large White pigs. Large White pigs had higher monounsaturated fatty acids and lower polyunsaturated fatty acids than Mashen pigs. The expression of MyHCI was significantly higher and the expression of MyHCIIb was lower in Mashen than in Large White pigs. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 56%

Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

Guo

Qin

Yang

et al. 2019

Italian Journal of Animal Science

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“…It has been reported that the IMF of European pigs is 2% to 3%, while in Chinese indigenous pigs it is always 4%, and in Min pig it could achieve 5%. Some studies suggest that high quality pork contains high IMF [ 20 , 21 ]. Thus, an effective means to increase meat quality is increasing IMF, which impels us to find out the underlying mechanism.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics Analysis on Excellent Meat Quality Traits of Skeletal Muscles of the Chinese Indigenous Min Pig Compared with the Large White Breed

Liu

Yang

Jing

et al. 2017

IJMS

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The Min pig (Sus scrofa) is a well-known indigenous breed in China. One of its main advantages over European breeds is its high meat quality. Additionally, different cuts of pig also show some different traits of meat quality. To explore the underlying mechanism responsible for the differences of meat quality between different breeds or cuts, the longissimus dorsi muscle (LM) and the biceps femoris muscle (BF) from Min and Large White pigs were investigated using transcriptome analysis. The gene expression profiling identified 1371 differentially expressed genes (DEGs) between LM muscles from Min and Large White pigs, and 114 DEGs between LM and BF muscles from the same Min pigs. Gene Ontology (GO) enrichment of biological functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the gene products were mainly involved in the IRS1/Akt/FoxO1 signaling pathway, adenosine 5′-monophosphate-activated protein kinase (AMPK) cascade effects, lipid metabolism and amino acid metabolism pathway. Such pathways contributed to fatty acid metabolism, intramuscular fat deposition, and skeletal muscle growth in Min pig. These results give an insight into the mechanisms underlying the formation of skeletal muscle and provide candidate genes for improving meat quality. It will contribute to improving meat quality of pigs through molecular breeding.

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“…4). And the results of GO and KEGG analyses revealed that these proteins were enriched in the protein binding, muscle contraction and energy metabolism pathways which took part in the regulation in the process of embryonic skeletal muscle development and growth of sheep [31][32][33][34][35] . And the cytoskeletal proteins with higher abundance in stage of muscle fibers proliferation and the energy metabolism played a major role in the embryonic muscle hypertrophy 13,36 , this indicated that D85N to D105N was the stage of embryonic muscle fibers proliferation and D105N to D135N was hypertrophy.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analyses of sheep (ovis aries) embryonic skeletal muscle

Wang

Shi

Zhao

et al. 2020

Sci Rep

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The growth and development of embryonic skeletal muscle plays a crucial role in sheep muscle mass. But proteomic analyses for embryonic skeletal development in sheep had been little involved in the past research. In this study, we explored differential abundance proteins during embryonic skeletal muscle development by the tandem mass tags (TMT) and performed a protein profile analyses in the longissimus dorsi of Chinese merino sheep at embryonic ages Day85 (D85N), Day105 (D105N) and Day135 (D135N). 5,520 proteins in sheep embryonic skeletal muscle were identified, and 1,316 of them were differential abundance (fold change ≥1.5 and p-value < 0.05). After the KEGG enrichment analyses, these differential abundance proteins were significant enriched in the protein binding, muscle contraction and energy metabolism pathways. After validation of the protein quantification with the parallel reaction monitoring (PRM), 41% (16/39) significant abundance proteins were validated, which was similar to the results of protein quantification with TMT. All results indicated that D85N to D105N was the stage of embryonic muscle fibers proliferation, while D105N to D135N was the stage of their hypertrophy. These findings provided a deeper understanding of the function and rules of proteins in different phases of sheep embryonic skeletal muscle growth and development.

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Integrative transcriptomics and proteomics analysis of longissimus dorsi muscles of Canadian double-muscled Large White pigs

Cited by 10 publications

References 65 publications

Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

Transcriptomics Analysis on Excellent Meat Quality Traits of Skeletal Muscles of the Chinese Indigenous Min Pig Compared with the Large White Breed

Proteomic analyses of sheep (ovis aries) embryonic skeletal muscle

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