Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response to Magnaporthe oryzae reveals different expression patterns at the molecular level of durably resistant rice cultivar Mowanggu

Abstract

Rice blast, caused by Magnaporthe oryzae is one of the most destructive diseases of rice (Oryza sativa L.) in most rice-cultivated areas worldwide. Mowanggu (MWG) is a traditional landrace rice variety in Yunnan with broad-spectrum and durable blast resistance against rice blast fungus. However, the underlying disease-resistance mechanisms remain unknown. An integrative transcriptomic, proteomic, and phosphoproteomic analysis of MWG was performed after inoculation with M. oryzae in this study. The transcriptomic and proteomic results revealed that MWG was moderately correlated at the transcriptional and protein levels. Differentially expressed genes and proteins were up-regulated and significantly enriched in protein phosphorylation, peroxisome, plant-pathogen interactions, phenylpropanoid metabolism and phenylalanine biosynthesis pathways. The phosphoproteomic profile and phosphorylated-protein-interaction network revealed that the altered phosphoproteins were primarily associated with reactive oxygen species (ROS), glycolysis, MAPK signaling pathways, and amino acid biosynthesis. In addition, a series of physiological and biochemical parameters, including ROS, soluble sugars, soluble protein and callus accumulation and defense-related enzyme activities, were used to validate the possible blast resistance mechanisms of MWG. The integrative transcriptomic, proteomic, and phosphoproteomic analysis revealed the different expression patterns at the molecular level of the durably resistant rice cultivar MWG after inoculation with M. oryzae, which provides insight into the molecular mechanisms of rice blast resistance.

Transcriptomic–Proteomic Analysis Revealed the Regulatory Mechanism of Peanut in Response to Fusarium oxysporum

“…In this study, the activities of PAL and 4CL increased significantly 2 d after inoculation, indicating that the inoculation of F. oxysporum promoted the process of phenylpropanoid metabolism pathways, promoted the metabolism of some phenolics, and increased the content of TF to fight against the disease. This is also similar to the determination related to the disease course of rice blast [45,46]. However, the results of decreased C4H activity are different from other reports [47].…”

Chilling stress response in tobacco seedlings: insights from transcriptome, proteome, and phosphoproteome analyses

“…Among them, PEX5 increased significantly, while PEX16 and PEX14 decreased at the levels of phosphopeptide abundance. This pathway was also significantly enriched in the rice variety MWG, which is resistant to Magnaporthe oryzae , the causal agent of rice blast, when the plant has been inoculated with this fungus ( Peng et al., 2023 ). Peroxisomes are dynamic organelles that are enveloped in membranes, and they enable the cells to rapidly respond to changing conditions.…”

“…Despite some progress in elucidating the molecular mechanisms used by tobacco to tolerate chilling stress, there is limited understanding of the post-translational phosphorylation modifications of proteins that are mediated by this type of stress. Currently, the comprehensive analysis of two or three types of omics data provides an important way to elucidate the regulatory mechanisms that plants use to respond to biotic or abiotic stress ( Hu et al., 2022 ; Peng et al., 2023 ). Samples of tobacco seedlings were analyzed at the transcriptomic, proteomic and phosphoproteomic levels to determine the global alteration of genes and proteins and the levels of their phosphorylation from 24 h of chilling treatment.…”