Integrative transcriptomic profiling reveals the key pathways in the regulation mechanism of fish intestine-spleen immunity during the bacterial challenges

Gao, Chengbin; Cai, Xin; Lymbery, A.J.; Ma, Le; Cao, Min; Li, Chao

doi:10.1016/j.aquaculture.2023.739320

Cited by 3 publications

(3 citation statements)

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“…In this study, 16 pathways related to immune response have been The activation of the 'intestinal immune network for IgA production pathway' has allowed the intestine to secrete large amounts of IgA antibodies to neutralize external antigens and toxins (Gao et al, 2023;Løkka & Koppang, 2016). Some studies have found that the induction of the 'intestinal immune network for IgA production pathway' was suppressed mainly by the activation of F I G U R E 9 Validation of the expression levels of 10 randomly selected immunerelated DEGs in RNA-seq data and qPCR data.…”

Section: Discussionmentioning

confidence: 99%

“…The activation of the ‘intestinal immune network for IgA production pathway’ has allowed the intestine to secrete large amounts of IgA antibodies to neutralize external antigens and toxins (Gao et al., 2023; Løkka & Koppang, 2016). Some studies have found that the induction of the ‘intestinal immune network for IgA production pathway’ was suppressed mainly by the activation of the ‘MAPK signaling pathway’ (Li, Hu, et al., 2020) and ‘calcium signaling pathway’ (Wang et al., 2022).…”

Section: Discussionmentioning

confidence: 99%

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Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

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Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed‐based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis and aeromoniasis in Oreochromis sp. However, the mechanisms of immunities in vaccinated fish still need clarification. Here, the hindgut transcriptome of vaccinated and control fish was determined, as the gut displays higher affinity towards antigen uptake and nutrient absorption. The efficacy of FBBV to improve fish immunity was evaluated according to the expression of immune‐related genes in the vaccinated fish hindgut throughout the 8‐week experimental period using RT‐qPCR. The vaccinated fish hindgut at week 6 was further subjected to transcriptomic analysis due to the high expression of immune‐related genes and contained killed whole organisms. Results demonstrated the expression of immune‐related genes was in correlation with the presence of killed whole organisms in the vaccinated fish hindgut. Transcriptomic analysis has allowed the prediction of robust immune‐related pathways, including innate and adaptive immunological responses in vaccinated fish hindgut than control fish. Pathways related to the regulation of lipid metabolism and modulation of the immune system were also significantly enriched (p ≤ .05). Overall, results offer a fundamental study on understanding the immunological response in Oreochromis sp. following vaccination with the FBBV pellet and support further application to prevent bacterial diseases in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Recent studies demonstrated that the spleen serves antibacterial functions in various fish species, with a high quantity of melano-macrophages that assist in phagocytosis during the immune response [47] . The rise of macrophages and the change in the pathological and physiological circumstances in fish are correlated [48] .…”

Section: Discussionmentioning

confidence: 99%

Linalool’s Multifaceted Antimicrobial Potential: Unveiling its Antimicrobial Efficacy and Immunomodulatory Role AgainstSaprolegnia parasitica

Tang,

Zhong,

Tang

et al. 2024

Preprint

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Saprolegnia parasitica(S.parasitica) is one of the most virulent oomycete species in freshwater aquatic environments, causing severe saprolegniasis and leading to significant economic losses in the aquaculture industry. Thus far, the prevention and control of saprolegniasis face a shortage of medications. Linalool, a natural antibiotic alternative found in various essential oils, exhibits promising antimicrobial activity against a wide range of pathogens. In this study, the specific role of linalool in protectingS.parasiticainfection at bothin vitroandin vivolevels was investigated. Linalool showed multifaceted anti-oomycetes potential by both of antimicrobial efficacy and immunomodulatory efficacy. Forin vitrotest, linalool exhibited strong anti-oomycetes activity and mode of action included: (1) Linalool disrupted the cell membrane of the mycelium, causing the intracellular components leak out; (2) Linalool prohibited ribosome function, thereby inhibiting protein synthesis and ultimately affecting mycelium growth. Surprisingly, meanwhile we found the potential immune protective mechanism of linalool in thein vivotest: (1) Linalool enhanced the complement and coagulation system which in turn activated host immune defense and lysateS.parasiticacells; (2) Linalool promoted wound healing, tissue repair, and phagocytosis to cope withS.parasiticainfection; (3) Linalool positively modulated the immune response by increasing the abundance of beneficial Actinobacteriota; (4) Linalool stimulated the production of inflammatory cytokines and chemokines to lyseS.parasiticacells. In all, our findings showed that linalool possessed multifaceted anti-oomycetes potential which would be a promising natural antibiotic alternative to cope withS.parasiticainfection in the aquaculture industry.

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The impact of Lactococcus lactis KUST48 on the transcription profile of Aeromonas hydrophila -infected zebrafish spleen

Chen,

Li,

Fan

et al. 2024

Microbiol Spectr

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Aeromonas hydrophila , an aquatic pathogenic bacterium, has been found to infect many fish species and cause huge aquaculture losses. Antibiotics are the most common drugs used to treat these infections. However, antibiotic abuse can lead to the development of antibiotic resistance. Probiotics have the potential to replace antibiotics for preventing infections. Zebrafish ( Danio rerio ) is a model organism used to study the innate immune system and host-pathogen interactions. Currently, there is little information on how the fish immune system responds to A. hydrophila and probiotic treatment. To increase the understanding of the molecular mechanisms behind the zebrafish defense against A. hydrophila and provide evidence that antibiotics can be replaced by probiotics, a transcriptome analysis of the zebrafish spleen was conducted 48 hours after infection by A. hydrophila , as well as after treatment using Lactococcus lactis KUST48 4 hours after infection. A total of 36,499 genes were obtained. There were 3,337 genes found to have significant differential expression between treatment and control groups. According to further annotation and enrichment analysis, differentially expressed genes (DEGs) were involved in signal transduction, endocrine system cancer, and the immune system. Insulin resistance disappeared in the zebrafish after treatment. Quantitative real-time PCR was performed to confirm the significant regulation of immune defense DEGs, the results of which were consistent with the RNA-sequencing data. These results could serve as a basis for future studies on the immune response to A. hydrophila and provide suggestions for probiotic alternatives to antibiotics, which will be of great significance to aquaculture and environmental protection. IMPORTANCE In recent years, the unreasonable use of antibiotics has led to the emergence of drug-resistant pathogenic bacteria, antibiotic residues, cross infection, toxic side effects, and so on, which has caused a serious threat to human food safety and life health. In recent years, many studies have demonstrated the potential of probiotics as a substitute for antibiotics, but there is still a lack of understanding of the molecular mechanisms underlying probiotic therapy. We conduct a research on the impact of Lactococcus lactis KUST48 on the transcription profile of Aeromonas hydrophila -infected zebrafish spleen. Mortality of zebrafish infected with A. hydrophila was significantly reduced after treatment with L. lactis KUST48. Our results can help to strengthen our understanding of the pathogenic mechanisms of zebrafish and provide a valuable reference for the molecular mechanisms of probiotic therapy.

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Integrative transcriptomic profiling reveals the key pathways in the regulation mechanism of fish intestine-spleen immunity during the bacterial challenges

Cited by 3 publications

References 75 publications

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Linalool’s Multifaceted Antimicrobial Potential: Unveiling its Antimicrobial Efficacy and Immunomodulatory Role AgainstSaprolegnia parasitica

The impact of Lactococcus lactis KUST48 on the transcription profile of Aeromonas hydrophila -infected zebrafish spleen

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