This paper presents faunistic records of spiders in the forest ecosystems of the Třesín National Nature Monument. Spiders were surveyed from 29 April 2013 to 25 October 2013. A total of 1012 individual spiders were collected from eight sites by pitfall traps, individual collection, sweeping grasses and herb vegetation, beating shrubs and trees, and shi ing leaf litters. Spiders were identifi ed as 146 species from 94 genera and 27 families. The families Linyphiidae, Lycosidae, Gnaphosidae, and Thomisidae exhibited high species diversity. Three species listed on the Red List of Threatened Species in the Czech Republic were recorded: Dysdera moravica (Řezáč, Gasparo, Král & Heneberg, 2014), Megalepthyphantes pseudocollinus (Saaristo, 1997), and Nusoncus nasutus (Schenkel, 1925). The fi nding of N. nasutus is among the fi rst reports of this spider in the Moravia region. Several fi ndings represent the northernmost occurrences of rare thermophilous spiders in Moravia and even the Czech Republic. The great richness of araneofauna and the occurrence of rare and poorly known spider species confi rm the high biotic value of Třesín within the agriculturally intensifi ed landscape of Moravia.