2018
DOI: 10.1038/nature26003
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Integrative structure and functional anatomy of a nuclear pore complex

Abstract: Summary Despite the central role of Nuclear Pore Complexes (NPCs) as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm, their large size and dynamic nature have impeded a full structural and functional elucidation. Here, we have determined a subnanometer precision structure for the entire 552-protein yeast NPC by satisfying diverse data including stoichiometry, a cryo-electron tomography map, and chemical cross-links. The structure reveals the NPC’s functional elements in unprecede… Show more

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Cited by 484 publications
(991 citation statements)
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“…NPC might be the perfect example to observe both these evolutionary forces acting at the same time and thus leading to a species‐specific interaction network. To support our view, two recent reports on tomography structure of NPC from Chlamydomonas reinhardtii and S. cerevisiae depict the architectural differences in the arrangement of major subcomplexes as compared to the H. sapiens NPC. Hence, it is important to elucidate the structural information of NPC in species‐specific context to fully understand its transport functions.…”
Section: Resultssupporting
confidence: 74%
“…NPC might be the perfect example to observe both these evolutionary forces acting at the same time and thus leading to a species‐specific interaction network. To support our view, two recent reports on tomography structure of NPC from Chlamydomonas reinhardtii and S. cerevisiae depict the architectural differences in the arrangement of major subcomplexes as compared to the H. sapiens NPC. Hence, it is important to elucidate the structural information of NPC in species‐specific context to fully understand its transport functions.…”
Section: Resultssupporting
confidence: 74%
“…3,22 Nup35 is a component of the Nup93 complex, which is the major structural unit of the inner ring, and interacts with Nups in the same complex (Nups93, 155, and 205) as well as other Nups (Nup98 and Ndc1) and the membrane. 3,22 Nup35 is a component of the Nup93 complex, which is the major structural unit of the inner ring, and interacts with Nups in the same complex (Nups93, 155, and 205) as well as other Nups (Nup98 and Ndc1) and the membrane.…”
Section: Discussionmentioning
confidence: 99%
“…Molecular traffic across the NE is controlled by nuclear pore complexes (NPCs), ~100 MD protein channels in Metazoans (~50 MD in budding yeast) that are formed by concentric inner, outer and membrane ring assemblies that scaffold a central transport channel, cytosolic filaments and nuclear basket (Figure 1)[7,8]. These architectural units are themselves constructed from modular subcomplexes of proteins termed nucleoporins or nups that are assembled step-wise into the NE, with an emerging role for unstructured motifs that connect subcomplexes together to help build the NPC[810].…”
Section: Introductionmentioning
confidence: 99%
“…These architectural units are themselves constructed from modular subcomplexes of proteins termed nucleoporins or nups that are assembled step-wise into the NE, with an emerging role for unstructured motifs that connect subcomplexes together to help build the NPC[810]. There remains considerable interest in understanding the biochemical and morphological differences that define post-mitotic and interphase modes of NPC assembly[1113].…”
Section: Introductionmentioning
confidence: 99%
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