Integrative SMRT sequencing and ginsenoside profiling analysis provide insights into the biosynthesis of ginsenoside in Panax quinquefolium

A systematic review of ginsenoside biosynthesis, spatiotemporal distribution, and response to biotic and abiotic factors in American ginseng

“…74 Additionally, certain UGT and CYP450 genes have been identified as candidates involved in downstream ginsenoside biosynthesis in American ginseng, such as UGT917 , UGT913 , UGT716 , and UGT711 , which have been positively correlated with pseudoginsenoside F 11 , notoginsenoside R1, notoginsenoside R2, and pseudoginsenoside RT 5 in American ginseng. 75 Four cytochrome P450 enzymes (CYP450) have been identified as potential catalysts for hydroxylation at specific positions (C-16 and C-30) of the triterpene backbone in ginsenoside biosynthesis. Additionally, UDP-xylose synthase has been selected as a candidate enzyme that is likely involved in the biosynthesis of ginsenoside Rb3.…”

Mass spectrometry-based ginsenoside profiling: Recent applications, limitations, and perspectives

“…Such a large-scale multiomics study regarding Panax species has not yet been reported. However, small-scale studies integrating transcriptome and ginsenoside profiles were recently conducted and provided many insights into ginsenoside biosynthesis [ [83] , [84] , [85] , [86] , [87] ], the tissue-level distributions of ginsenosides and their biosynthesis [ 88 , 89 ], and the effects of microorganisms [ 90 , 91 ]. We anticipate that further multiomics studies with Panax species will be conducted in the near future.…”

Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius

“…For the expression patterns of different tissues, we used RNA-seq data that we reported previously [43]. We completed the transcription sequencing for the identification of MeJA-responsive PqMYBs.…”