Integrative Research of Induction of Pluripotent Stem Cells

Abstract

Since the Japanese scientist Shinya Yamanaka used a viral vector to transfer the combination of 4 factors into differentiated somatic cells and reprogramed them to obtain similar embryonic stem cells and induced pluripotent stem cells (iPSCs), it provided one integrative method for studying many medical fields. Patient-derived iPSCs have provided an opportunity to study human diseases for which no suitable model systems are available. iPSC technology has since become a major breakthrough in the field of stem cell research. With the continuous development of iPSC technology and the continuous improvement of technical levels, excellent advances have become more and more common in the basic research and medical fields of life sciences. This article reviews the development history, clinical application, and problems and prospects of iPSC, and focuses on the application of iPSC in neurological diseases.

Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency

“…Human iPSCs have been generated from various cell types and have a great potential for regenerative medicine, because they enable the derivation of patient‐specific pluripotent cells and serve as a platform for stem‐based research, disease modeling, and drug discovery/repurposing . Despite extensive research toward understanding of the reprogramming process, the underlying mechanisms are not fully understood , hindering their effective application in clinical studies . A number of molecular and cellular barriers of reprogramming have been identified to date , resulting in an overall 2%–5% efficiency, thus indicating that the majority of cells are unable to complete reprogramming toward pluripotency .…”