Integrative proteomics to understand the transmission mechanism of Barley yellow dwarf virus-GPV by its insect vector Rhopalosiphum padi

Wang, Hui; Wu, Keke; Liu, Yan; Wu, Yunfeng; Wang, Xifeng

doi:10.1038/srep10971

Cited by 52 publications

(67 citation statements)

References 62 publications

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“…In addition, heritable aphid and endosymbiont proteins are found to be implicated in the transmission of circulative polerovirus (Cilia et al ., ). Integrative proteomics can be used to uncover the mechanism of barley yellow dwarf virus‐GPV by the aphid Rhopalosiphum padi (Wang et al ., ). Furthermore, proteomic studies of the interaction between rice stripe virus and the vector, the small brown planthopper ( Laodelphax striatellus ), provide evidence for new functions of a hemipetran cuticular protein (Liu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Killiny

2017

Physiol. Entomol

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Asian citrus psyllid Diaphorina citri is the vector of the citrus Huanglongbing (HLB) associated bacterial agent ‘Candidatus Liberibacter asiaticus’ (CLas). The molecular interactions between CLas and D. citri remain unclear. In the present study, protein profiles of mitochondrial, microsomal and cytosolic fractions from uninfected and CLas‐infected adult D. citri are investigated using two‐dimensional gel electrophoresis. The comparative analysis reveals a total of 18, 24 and 20 protein spots that are unique or differentially expressed in mitochondrial, microsomal and cytosolic proteins fractions respectively. These proteins are successfully identified by mass spectrometry. Among the 62 identified proteins, 30 are up‐regulated, whereas 32 are down‐regulated. These proteins include important components in energy metabolism such as ATP synthase, ATPase, ATP/ADP carrier protein, etc.; host stress responses such as heat shock proteins; host detoxification processes (i.e., cytochrome P450 and glutathione S‐transferase); and the cytoskeleton (such as actin, tubulin, myosin and tropomyosin). These data suggest that, after CLas infection, several proteins of D. citri, especially energy metabolism and protein biosynthesis, are altered, and extensive host defence responses are induced. In conclusion, the present study reports proteomic information that is helpful in understanding the vector–pathogen relationship between CLas and D. citri, and could be used to identify potential targets for limiting the spread of CLas, as well as to provide new insights into HLB management.

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Section: Discussionmentioning

confidence: 97%

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Killiny

2017

Physiol. Entomol

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“…Protein function classification was based on the annotation from UniProt67. The subcellular localization was the integration of prediction results through WoLF PSORT (http://www.genscript.com/wolf-psort.html), Plant-mPLoc (http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/)68 and UniprotKB.…”

Section: Methodsmentioning

confidence: 99%

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.Plants encounter a variety of biotic and abiotic stresses during growth 1 . These stresses unbalance cellular homeostasis and lead to morphological, physiological, and molecular changes 2 . These changes have a negative impact on survival and biomass production, and can reduce final yield by up to 82% 3 . Global warming and climate change may also be exacerbating the effects of abiotic stresses on crop production in many areas of the world today. Previous reports have suggested that a temperature increase of 1 °C can produce a decrease in yield of up to 10% 4 . Drought in particular, severely impairs plant growth and development and limits crop productivity more than any other environmental factor 5,6 . As the climate continues to change, drought may become a more frequent and cause severe problem 7 . Drought stress induces a range of physiological and biochemical responses in plants. Under drought conditions, the plant root cap produces abscisic acid (ABA), which mediates stomatal closure. This in turn, suppresses cell growth, photosynthetic efficiency, and respiration [8][9][10] . Recently, ABA and stomata have been implicated in the regulation of systemic responses to abiotic stresses 11 . In addition, plants generate toxic ions and reactive oxygen species (ROS) that can decrease enzyme activities and damage essential proteins 12 . The generation of ROS occurs early in the response to water-stress, and ROS are key secondary messengers triggering subsequent defensive measures in plants 1,[13][14][15] . Plants have developed a sophisticated and elaborate system for scavenging high levels of ROS using antioxidant enzymes that include superoxid...

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“…A CP of the hemipteran vector, Laodelphax striatellus , was found to interact with the nucleocapsid protein (pc3) of Rice stripe virus (genus Tenuivirus , family Phenuiviridae ) and was hypothesized to be involved in viral transmission and to possibly protect the virus from degradation by a host immune response in the hemolymph (19). Recently, a CP of another hemipteran vector, Rhopalosiphum padi , was identified to interact with Barley yellow dwarf virus-GPV (genus Luteovirus , family Luteoviridae ) readthrough protein, and the gene transcript of this particular CP was differentially expressed in viruliferous compared to virus-free aphids (38). At the transcript level, thrips cuticular proteins of different types - including the thrips CPRs used in the present study to phylogenetically place the three cuticular TIPs - were identified to be downregulated in TSWV-infected first instar larvae (18).…”

Section: Discussionmentioning

confidence: 99%

Discovery of novel thrips vector proteins that bind to the viral attachment protein of the plant bunyavirus, tomato spotted wilt virus

Badillo-Vargas¹,

Chen

Martin

et al. 2018

Preprint

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16The plant-pathogenic virus, tomato spotted wilt virus (TSWV), encodes a structural 17 glycoprotein (G N ) that, like with other bunyavirus/vector interactions, serves a role in viral 18 attachment and possibly entry into arthropod vector host cells. It is well documented that 19Frankliniella occidentalis is one of seven competent thrips vectors of TSWV transmission to 20 plant hosts, however, the insect molecules that interact with viral proteins, such as G N , during 21 infection and dissemination in thrips vector tissues are unknown. The goals of this project 22 Importance Statement 37 Thrips-transmitted viruses cause devastating losses to numerous food crops worldwide. For 38 negative-sense RNA viruses that infect plants, the arthropod serves as a host as well by 39 supporting virus replication in specific tissues and organs of the vector. The goal of this work 40 was to identify vector/host proteins that bind directly to the viral attachment protein and thus 41 may play a role in the infection cycle in the insect. Using the model plant bunyavirus, tomato 42 spotted wilt virus (TSWV), and the most efficient thrips vector, we identified and validated 43 six TSWV-interacting proteins from Frankliniella occidentalis first instar larvae. Two 44 proteins, an endocuticle structural glycoprotein and cyclophilin, were able to interact directly 45 with the TSWV attachment protein, G N, in insect cells. The TSWV G N -interacting proteins 46 3 provide new targets for disrupting the virus-vector interaction and could be putative 47 determinants of vector competence. 48 49Vector-borne diseases caused by animal-and plant-infecting viruses are some of the 50 most important medical, veterinary, and agricultural problems worldwide (1, 2). The majority 51 of viruses infecting plants and animals are transmitted by arthropods. Understanding the viral 52 and arthropod determinants of vector competence is important for basic knowledge of virus-53 vector interactions and development of new interdiction strategies to control disease. 54Significant progress has been made towards identification of viral determinants of 55 transmission, but the interacting molecules in vectors remain largely elusive. For negative-56 sense RNA viruses, vector factors that mediate the transmission process have not been well 57 characterized. 58Bunyavirales is the largest order of negative-sense RNA viruses; twelve families are 59 described (http://www.ictvonline.org/virustaxonomy.asp). The Bunyavirales contains plant 60 and insect vector-infecting viruses that make up the Family Tospoviridae (3-5). Within this 61 family, there are eighteen species and several unassigned viruses that most likely will be 62 classified as unequivocal members of the Orthotospovirus genus. Tomato spotted wilt 63 orthotospovirus is the type species within this genus and has been best characterized in terms 64 of viral host range, genome organization and protein functions (6, 7). 65 Tomato spotted wilt virus (TSWV) infects both monocotyledonous and dicotyledonous 66 plants enc...

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Integrative proteomics to understand the transmission mechanism of Barley yellow dwarf virus-GPV by its insect vector Rhopalosiphum padi

Cited by 52 publications

References 62 publications

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Huanglongbing pathogen CandidatusLiberibacter asiaticus exploits the energy metabolism and host defence responses of its vector Diaphorina citri

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Discovery of novel thrips vector proteins that bind to the viral attachment protein of the plant bunyavirus, tomato spotted wilt virus

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