Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming Crab Portunus trituberculatus

Meng, Xianliang; Zhang, Mengqian; Gao, Bingbo; Lv, Jianjian; Li, Jian; Ping, Liu

doi:10.3389/fendo.2020.00533

Cited by 14 publications

(9 citation statements)

References 71 publications

(76 reference statements)

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“…β-actin was used as the internal control. Specific primers were designed based on gene sequences from our previous transcriptome data or previous studies (Zhang et al, 2009;Cui et al, 2010;Meng et al, 2014Meng et al, , 2015Meng et al, , 2020Ren et al, 2016; Table 1). The reactions were performed with the following program: 95 • C for 30 s; 94 • C for 5 s, and 60 • C for 15 s (35-40 cycles).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Long-Term Ammonia Toxicity in the Hepatopancreas of Swimming Crab Portunus trituberculatus: Cellular Stress Response and Tissue Damage

Zhang

Cao

et al. 2022

Front. Mar. Sci.

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Ammonia is the most common contaminant in aquaculture systems. Due to the unexpected deterioration of natural seawater quality caused by harmful alga blooms in summer, the water exchange cycle for the culture ponds of the swimming crab Portunus trituberculatus, an important aquaculture species in China, is often much longer, and the crabs may be exposed to long-term ammonia stress. However, less information is available regarding the long-term effects of ammonia in marine decapod crustaceans. Therefore, it is of great significance to understand the toxic effects of prolonged ammonia in decapods. In this study, alteration of histology, cellular stress responses (CSRs), and apoptosis in the hepatopancreas of P. trituberculatus, an important aquaculture species, during ammonia stress (5, 15, and 45 mg⋅L–1) for 1, 7, and 15 days and recovery at the normal condition for 7 days, were analyzed. Our results demonstrated a serious impact of long-term (15 days) ammonia stress by depressing crab CSR especially when ammonia concentration exceeds 15 mg⋅L–1. Overall, short- (1 day) and medium-term (7 days) ammonia stress induced CSR, evidenced by upregulated expression of the genes involved in antioxidant defense (SOD, CAT, and GPX), apoptosis (p53, Bax, and Caspase-3), heat shock response (HSR) (Hsp70 and Hsp90), unfolded protein response (UPR) (IRE1, ATF6, and XBP1), and DNA damage response (DDR) (ATR and DNA-PKcs). However, long-term (15 days) ammonia stress, especially when exposed to the ammonia of 15 and 45 mg⋅L–1, resulted in a higher level of apoptosis and severe damage of hepatopancreas, which may be related to the depressed CSR including antioxidant defense, HSR, UPR, and DDR. Notably, after recovery, the expressions of many genes involved in apoptosis, antioxidant response, HSR, UPR, and DDR in the groups exposed to ammonia at 15 and 45 mg⋅L–1 were still significantly different to that of the control group. In summary, care should be taken when P. trituberculatus is exposed to ammonia over 15 mg⋅L–1 especially when exposure duration is longer than 15 days, as the CSR could be compromised. This study provides a reference for a comprehensive understanding of CSR in decapod crustaceans under ammonia stress and will be beneficial for management in the intensive culture of the swimming crab.

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Long-Term Ammonia Toxicity in the Hepatopancreas of Swimming Crab Portunus trituberculatus: Cellular Stress Response and Tissue Damage

Zhang

Cao

et al. 2022

Front. Mar. Sci.

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“…miRNAs are also essential for ovulation and oocyte maturation in invertebrates (Kadener et al, 2009). In decapods, several sexrelated miRNAs have been identified in S. paramamosain (Jia et al, 2018;Waiho et al, 2019a;Zhou et al, 2019Zhou et al, , 2020, Portunus trituberculatus (Meng et al, 2018(Meng et al, , 2020, E. sinensis (Song et al, 2014;He et al, 2015), M. rosenbergii (Liu X. et al, 2019;Qiao et al, 2019) M. nipponense (Sb et al, 2015;Jin et al, 2017), P. monodon (Zhao et al, 2018), and Fenneropenaeus merguiensis (Saetan and Chotigeat, 2019). The roles of sex-related miRNAs in decapods are summarized in Table 3.…”

Section: Non-coding Rnas (Ncrnas)mentioning

confidence: 99%

“…In addition, the expression level of miR-87 and miR-7 was significantly increased and they were predicted to target cyclin-dependent kinase 2 (Cdk2)7 and cell division cycle4 (Cdc4), which are important for regulation of the G1-to-S-phase transition during the cell cycle (Schnackenberg et al, 2007). Meng et al (2020) showed that MF synthesis and FAMeT expression are directly regulated by miR-4171 and miR-263a. Also, miR-317, miR-2b, and miR-466f-3p repress the expression of calcium signal transduction (CaM).…”

Section: Non-coding Rnas (Ncrnas)mentioning

confidence: 99%

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

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Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-related genes (i.e., CFSH, DEAD-box family, Tra-2, Sxl, Dsx, Fem-1, Sox gene family, Foxl2, and Dmrt gene family) have been identified via transcriptomic analysis in crustaceans, indicating that sexual differentiation in crustaceans is more complicated than previously expected. It has been found that several non-coding RNAs (i.e., miRNAs, lncRNAs, and piRNAs) and IAG receptors may be involved in the sexual development of decapods. Identification and study of the regulation mechanism of sex-related genes, non-coding RNAs, and IAG receptors will provide valuable information regarding sexual development in decapods. In this review, the roles of hormonal and genetic factors in both males and females are discussed. In males, crustacean female sex hormone (CFSH), Sxl, Dmrt gene family, Dsx, Sox gene family, GEM, Fem-1, l-GnRH-III, and corazonin play important roles in IAG regulation in the “eyestalk-IAG-testis” endocrine axis. Unlike males, the regulation mechanism and interaction of sexual genes are relatively unknown in females. However, CFSH, IAG, Fem-1, FAMeT, Slo, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH seem to play crucial roles during ovarian development. This study summarizes the available information in the field, highlights gaps, and lays the foundations for further studies and a better understanding of the regulatory mechanism of sexual development in decapods.

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“…The swimming crab, Portunus trituberculatus (Crustacea: Decapoda: Brachyura) is common edible crab. It is widely distributed in the estuary and coastal waters of Korea, Japan, China, and Southeast Asia ( Meng et al., 2020 ). The only neuropeptides reported in this species include CHH ( Xie et al., 2014 ), MIH ( Wang et al., 2013 ), and CCAP ( Sun et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Wang

et al. 2021

PeerJ

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Neuropeptides and their G protein-coupled receptors (GPCRs) regulate multiple physiological processes. Currently, little is known about the identity of native neuropeptides and their receptors in Portunus trituberculatus. This study employed RNA-sequencing and reverse transcription-polymerase chain reaction (RT-PCR) techniques to identify neuropeptides and their receptors that might be involved in regulation of reproductive processes of P. trituberculatus. In the central nervous system transcriptome data, 47 neuropeptide transcripts were identified. In further analyses, the tissue expression profile of 32 putative neuropeptide-encoding transcripts was estimated. Results showed that the 32 transcripts were expressed in the central nervous system and 23 of them were expressed in the ovary. A total of 47 GPCR-encoding transcripts belonging to two classes were identified, including 39 encoding GPCR-A family and eight encoding GPCR-B family. In addition, we assessed the tissue expression profile of 33 GPCRs (27 GPCR-As and six GPCR-Bs) transcripts. These GPCRs were found to be widely expressed in different tissues. Similar to the expression profiles of neuropeptides, 20 of these putative GPCR-encoding transcripts were also detected in the ovary. This is the first study to establish the identify of neuropeptides and their GPCRs in P. trituberculatus, and provide information for further investigations into the effect of neuropeptides on the physiology and behavior of decapod crustaceans.

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Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming Crab Portunus trituberculatus

Cited by 14 publications

References 71 publications

Long-Term Ammonia Toxicity in the Hepatopancreas of Swimming Crab Portunus trituberculatus: Cellular Stress Response and Tissue Damage

Long-Term Ammonia Toxicity in the Hepatopancreas of Swimming Crab Portunus trituberculatus: Cellular Stress Response and Tissue Damage

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

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