Integrative omics analysis reveals the protective role of vitamin C on perfluorooctanoic acid-induced hepatoxicity

Li, Rong; Guo, Chao; Lin, Xiao; Chan, Ting‐Fung; Su, Min; Zhang, Zhiyong; Lai, Keng Po

doi:10.1016/j.jare.2021.04.003

Cited by 10 publications

(5 citation statements)

References 68 publications

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“…A number of studies have shown that PFCs disrupt the endocrine system in mammals [26]. For example, PFOA exposure interfered with the production of primary bile acids, steroids, and unsaturated fatty acids in mice [27]. Another in vitro study indicated that PFOA exposure significantly affected the expression of genes related to glucocorticoid and androgen biosynthesis in rat primary Sertoli cells [28].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Hepatotoxicity of Perfluorooctanoic Acid in Black-Spotted Frogs (Rana nigromaculata)

et al. 2022

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Perfluorooctanoic acid (PFOA) is frequently detected in the environment and accumulates in amphibians such as black-spotted frogs (Rana nigromaculata) with toxic effects; however, the mechanism underlying this toxicity is unclear. In this study, male black-spotted frogs were exposed to 10 μg/L waterborne PFOA for 21 days. Subsequently, the effect of PFOA exposure on gene expression in liver tissue was investigated using transcriptomic techniques. In total, 754 differentially expressed genes (DEGs; 389 up-regulated and 365 down-regulated) were identified. According to Kyoto Encyclopedia of Genes and Genomes enrichment and Gene Ontology functional enrichment analyses, the DEGs were mainly involved in lipid metabolism, endocrine functions, and immunity. Quantitative real-time polymerase chain reaction analysis of 15 selected DEGs revealed a high correlation (R2 = 0.9917) with the transcriptomic results. These results indicated that the PFOA hepatotoxicity in frogs is mediated mainly by lipid metabolism dysregulation, endocrine system disruption, and immunotoxicity. This study provides insights into the hepatotoxic mechanism of PFOA and other perfluorinated compounds in amphibians.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Hepatotoxicity of Perfluorooctanoic Acid in Black-Spotted Frogs (Rana nigromaculata)

et al. 2022

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“…The cerebral ischemic core area of the MCAO rats was white, and the brain tissue around the ischemic penumbra was regarded as the ischemic core area. Total RNA was extracted using TRIzol reagent, following the manufacturer's instructions, as previously described (12). Highquality RNA samples with an RNA integrity number > 7.0 were used to construct the sequencing library as previously described (12).…”

Section: Comparative Transcriptome Sequencing and Bioinformatic Analysismentioning

confidence: 99%

“…Total RNA was extracted using TRIzol reagent, following the manufacturer's instructions, as previously described (12). Highquality RNA samples with an RNA integrity number > 7.0 were used to construct the sequencing library as previously described (12). Paired-end sequencing (PE150) of 2 × 150 bp was performed using a DNBSEQ sequencer as the customer service provided by the Beijing Genomics Institute.…”

Section: Comparative Transcriptome Sequencing and Bioinformatic Analysismentioning

confidence: 99%

Molecular mechanisms for the prevention and promoting the recovery from ischemic stroke by nutraceutical laminarin: A comparative transcriptomic approach

et al. 2022

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Stroke is the second leading cause of death and a major cause of disability worldwide. Ischemic stroke caused by atherosclerosis accounts for approximately 87% of all stroke cases. Ischemic stroke is a preventable disease; therefore, a better understanding of the molecular mechanisms underlying its pathogenesis and recovery processes could provide therapeutic targets for drug development and reduce the associated mortality rate. Laminarin, a polysaccharide, is a nutraceutical that can be found in brown algae. Accumulating evidence suggests that laminarin could reduce the detrimental effects of neuroinflammation on brain damage after stroke. However, the molecular mechanism underlying its beneficial effects remains largely unknown. In the present study, we used a middle cerebral artery occlusion (MCAO) rat model and applied comparative transcriptomics to investigate the molecular targets and pathways involved in the beneficial effects of laminarin on ischemic stroke. Our results show the involvement of laminarin targets in biological processes related to blood circulation, oxygen supply, and anti-inflammatory responses in the normal brain. More importantly, laminarin treatment attenuated brain damage and neurodeficits caused by ischemic stroke. These beneficial effects are controlled by biological processes related to blood vessel development and brain cell death through the regulation of canonical pathways. Our study, for the first time, delineated the molecular mechanisms underlying the beneficial effects of laminarin on ischemic stroke prevention and recovery and provides novel therapeutic targets for drug development against ischemic stroke.

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“…There may be hyper or hyposecretions of digestive fluids owing to the feed taken by pigs in the digestion processes (Wenk, 2001). Some researchers proved that ascorbic acid a had protective role on the liver when perfluorooctanoic acid was used to induce hepatic steatosis (Li et al, 2022). Also, some researchers showed the ameliorative effect of ascorbic acid on the hepatotoxic effect of two analgesics (Al-Hayder et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Impact of Dietary Supplemental Ascorbic Acid on Organ Weight, Villi Morphometry, Organoleptic Attributes and Organ Histology of Weaned Pigs

Ojediran,

Afolabi,

Kehinde

et al. 2023

Malays. anim. husb. J.

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This study aimed at evaluating the impact of dietary supplemental ascorbic acid on organ weight, villi morphometry, organoleptic attributes, and organ histology of weaned pigs using thirty-two Large white-Landrace crossbred with an average weight of 12.32±0.59 kg. The pigs were grouped into four treatments with eight replicates each. Diet C0 was the basal diet (control). Diets C1, C2, and C3 contained the basal diet with 1g/kg, 2g/kg and 3g/kg of ascorbic acid respectively for seven weeks. The weight of liver, kidney, spleen, heart, and lungs were significantly different (p<0.05) with pigs offered ascorbic acid-supplemented diet having higher values. Also, the villus height, villus width and muscle thickness were significantly influenced (p<0.05) with pigs fed diet C3 having the highest value for villus height and width. The organoleptic properties did not differ significantly (p>0.05). The jejunum villi histology showed that pigs fed diets C0 and C1 were essentially normal while those fed diets C2 and C3 had moderate enteritis. No observable lesions were recorded in the structure of the nephron. Moreover, the hepatic histology of pigs fed diet C1 was essentially normal while those fed diets C2 and C3 showed normal hepatocytes but with moderate sinusoidal congestion. It can be concluded that ascorbic acid supplementation caused increased organ weight, aided villus height and width, and reduced muscle thickness with moderate sinusoidal congestion at up to 3g/kg with no deleterious effects on organoleptic attributes.

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Integrative omics analysis reveals the protective role of vitamin C on perfluorooctanoic acid-induced hepatoxicity

Abstract: Graphical abstract

Cited by 10 publications

References 68 publications

Transcriptomic Analysis Reveals the Hepatotoxicity of Perfluorooctanoic Acid in Black-Spotted Frogs (Rana nigromaculata)

Transcriptomic Analysis Reveals the Hepatotoxicity of Perfluorooctanoic Acid in Black-Spotted Frogs (Rana nigromaculata)

Molecular mechanisms for the prevention and promoting the recovery from ischemic stroke by nutraceutical laminarin: A comparative transcriptomic approach

Impact of Dietary Supplemental Ascorbic Acid on Organ Weight, Villi Morphometry, Organoleptic Attributes and Organ Histology of Weaned Pigs

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