Integrative Metabolomic Characterization Reveals the Mediating Effect of Bifidobacterium breve on Amino Acid Metabolism in a Mouse Model of Alzheimer’s Disease

Zhu, Guangsu; Guo, Min; Zhao, Jianxin; Zhang, Hao; Wang, Gang; Chen, Wei

doi:10.3390/nu14040735

Cited by 23 publications

(27 citation statements)

References 34 publications

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“…The system was used to detect metabolites in the host serum. The instruments’ operating conditions were set according to the method described by Zhu et al [ 17 ]. Briefly, a Waters Acquity UPLC T3 column (2.1 × 100 mm, 1.8 µm) was used for gradient elution at an operating temperature of 30 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The system was used to detect metabolites in the host serum. The instruments' operating conditions were set according to the method described by Zhu et al [17]. Briefly, a Waters Acquity UPLC T3 column (2.1 × 100 mm, 1.8 µm) was used for gradient elution at an operating temperature of 30 • C. Mobile phases of (A) 0.1% formic acid-water and (B) acetonitrile were used for positive ion scanning modes; (A) 5 mmol/L ammonium acetate aqueous solution and (B) acetonitrile were used for negative ion scanning modes.…”

Section: Lc/ms Non-targeted Metabolomics Analysismentioning

confidence: 99%

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Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Wang

et al. 2023

Nutrients

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Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the relationship between the gut microbiome and metabolites. This study used 16S rRNA microbial genomics and non-target metabolome based on liquid chromatography–mass spectrometry to analyze the gut microbiota composition and serum metabolic profiles of 30 FC patients and 28 healthy individuals. We found that patients with FC and healthy individuals have different gut microbiota structures and serum metabolic profiles. FC patients had more Bacteroides and butyrate-producing bacteria (Roseburia, Faecaliberium, Butyriccoccus). The upstream products of host arginine biosynthesis (2-oxoglutaric acid, L-glutamic acid, N-acetylornithine, and L-ornithine) were significantly reduced in FC patients’ serum metabolites. In summary, our study describes the gut microbiome and serum metabolome of patients with functional constipation. It reveals that functional constipation may be associated with increased Bacteroidetes and downregulation of upstream products of host arginine biosynthesis, which may be potential markers for diagnosing functional constipation.

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Section: Methodsmentioning

confidence: 99%

Section: Lc/ms Non-targeted Metabolomics Analysismentioning

confidence: 99%

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Wang

et al. 2023

Nutrients

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“…The detailed protocol for fecal metabolomics analysis, including metabolite sample preparation and the HPLC-MS analysis parameters are described in the Supplementary methods. The metabolomic data analysis were detailed in a previous report ( 19 ).…”

Section: Methodsmentioning

confidence: 99%

“…Bifidobacterium breve CCFM1025 is a promising psychobiotic strain that has been isolated from fecal samples of healthy humans ( 17 , 18 ). It has been shown to have several neuroprotective effects against AD and depression, and the underlying mechanisms involve the suppression of neuroinflammation, modulation of the microbiota, and the ability to colonize the gastrointestinal tract ( 19 , 20 ). The aim of this study was to investigate the effects of combined B. breve CCFM1025 with EE treatment on cognition and the gut microbiome and its metabolites in mice with AD.…”

Section: Introductionmentioning

confidence: 99%

Bifidobacterium breve intervention combined with environmental enrichment alleviates cognitive impairment by regulating the gut microbiota and microbial metabolites in Alzheimer’s disease mice

et al. 2022

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Alzheimer’s disease (AD) is characterized by behavioral and cognitive impairments and its increasing prevalence imposes a healthcare burden on society. To date, most intervention studies have only focused on a single AD-related factor and have yielded modest cognitive improvements. Here, we show that environmental enrichment (EE) training combined with Bifidobacterium breve CCFM1025 intervention significantly alleviated amyloid-β (Aβ)-induced cognitive impairment and inhibited neuroinflammation in mice. Moreover, we found that EE combined with B. breve CCFM1025 treatment restored AD-associated gut microbiota dysbiosis and reversed microbial metabolites changes. By integrating behavioral and neurological data with metabolomic profiles, we corroborated the microbiota–metabolite–brain interactions, with acetate and tryptophan metabolism as potential drivers. Taken together, our results provide a promising multidomain intervention strategy to prevent cognitive decline and delay the progression of AD through a combination of dietary microbiome-based approaches and lifestyle interventions.

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“…The metabolite samples were analysed using an ItiMateU-3000 ultraperformance liquid-chromatography (UPLC) system (Thermo Fisher Scientific, MA, USA) coupled to a high-resolution Q Exactive mass spectrometer (MS) (Thermo Fisher Scientific, MA, USA). The UPLC-MS analysis parameters and metabolomic data analysis have been described in a previous report [27].…”

Section: Metabolomicsmentioning

confidence: 99%

Environmental enrichment in combination with Bifidobacterium breve HNXY26M4 intervention amplifies neuroprotective benefits in a mouse model of Alzheimer's disease by modulating glutamine metabolism of the gut microbiome

Zhu¹,

Guo²,

Zhao³

et al. 2024

Food Science and Human Wellness

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The gut microbiota-brain axis has emerged as a novel target for Alzheimer's disease (AD), a neurodegenerative disease characterised by behavioural and cognitive impairment. However, most previous microbiome-based intervention studies have focused on single factors and yielded only modest cognitive improvements. Here, we proposed a multidomain intervention strategy that combined Bifidobacterium breve treatment with environmental enrichment (EE) training. In this study, we found that compared with EE or B. breve treatment alone, B. breve intervention combined with EE amplified its neuroprotective effects on AD mice, as reflected by improved cognition, inhibited neuroinflammation and enhanced synaptic function. Moreover, using microbiome and metabolome profiling, we found that the combination of B. breve and EE treatment restored AD-related gut microbiota dysbiosis and reversed microbial metabolite changes. Finally, by integrating behavioural and neurological data with metabolomic profiles, we revealed that the underlying mechanism may involve the modulation of microbiota-derived glutamine metabolism via gut-brain interactions. Collectively, combined B. breve intervention with EE treatment can alleviate AD-related cognitive impairment and improve brain function by regulating glutamine metabolism of the gut microbiome. Our findings provide a promising multidomain intervention strategy, with a combination of dietary microbiome-based and lifestyle-targeted interventions, to promote brain function and delay the progression of AD.

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Integrative Metabolomic Characterization Reveals the Mediating Effect of Bifidobacterium breve on Amino Acid Metabolism in a Mouse Model of Alzheimer’s Disease

Cited by 23 publications

References 34 publications

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Bifidobacterium breve intervention combined with environmental enrichment alleviates cognitive impairment by regulating the gut microbiota and microbial metabolites in Alzheimer’s disease mice

Environmental enrichment in combination with Bifidobacterium breve HNXY26M4 intervention amplifies neuroprotective benefits in a mouse model of Alzheimer's disease by modulating glutamine metabolism of the gut microbiome

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