Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies

Cortese, Mirko; Lee, Ji Young; Cerikan, Berati; Neufeldt, Christopher J; Oorschot, Viola; Köhrer, Sebastian; Hennies, Julian; Schieber, Nicole L.; Ronchi, Paolo; Mizzon, Giulia; Romero-Brey, Inés; Santarella-Mellwig, Rachel; Schorb, Martin; Boermel, Mandy; Mocaer, Karel; Beckwith, Marianne Sandvold; Templin, Rachel; Gross, Viktoriia; Pape, Constantin; Tischer, Christian; Frankish, Jamie; Horvat, Natalie K.; Laketa, Vibor; Stanifer, Megan L.; Boulant, Steeve; Ruggieri, Alessia; Chatel‐Chaix, Laurent; Schwab, Yannick; Bartenschlager, Ralf

doi:10.1016/j.chom.2020.11.003

Cited by 235 publications

(319 citation statements)

References 45 publications

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“…In particular, COVID-altered cytoskeletal filament proteins shed light on viral trafficking in host cells. SARS-CoV-2 infection induces profound remodeling of the cytoskeleton, and replicating viral vesicles are surrounded by a network of intermediate filaments ( 72 ). The cytoskeletal network appears to facilitate coronavirus transport and expulsion, with thickening actin filaments providing the bending force to extrude viral vesicles ( 73 ).…”

Section: Resultsmentioning

confidence: 99%

An Autoantigen Atlas from Human Lung HFL1 Cells Offers Clues to Neurological and Diverse Autoimmune Manifestations of COVID-19

Wang¹,

Zhang

Mw³

et al. 2021

Preprint

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COVID-19 is accompanied by a myriad of both transient and long-lasting autoimmune responses. Dermatan sulfate (DS), a glycosaminoglycan crucial for wound healing, has unique affinity for autoantigens (autoAgs) from apoptotic cells. DS-autoAg complexes are capable of stimulating autoreactive B cells and autoantibody production. Using DS affinity, we identified an autoantigenome of 408 proteins from human fetal lung fibroblast HFL11 cells, at least 231 of which are known autoAgs. Comparing with available COVID data, 352 proteins of the autoantigenome have thus far been found to be altered at protein or RNA levels in SARS-Cov-2 infection, 210 of which are known autoAgs. The COVID-altered proteins are significantly associated with RNA metabolism, translation, vesicles and vesicle transport, cell death, supramolecular fibrils, cytoskeleton, extracellular matrix, and interleukin signaling. They offer clues to neurological problems, fibrosis, smooth muscle dysfunction, and thrombosis. In particular, 150 altered proteins are related to the nervous system, including axon, myelin sheath, neuron projection, neuronal cell body, and olfactory bulb. An association with the melanosome is also identified. The findings from our study illustrate a strong connection between viral infection and autoimmunity. The vast number of COVID-altered proteins with propensity to become autoAgs offers an explanation for the diverse autoimmune complications in COVID patients. The variety of autoAgs related to mRNA metabolism, translation, and vesicles raises concerns about potential adverse effects of mRNA vaccines. The COVID autoantigen atlas we are establishing provides a detailed molecular map for further investigation of autoimmune sequelae of the pandemic.Summary sentenceAn autoantigenome by dermatan sulfate affinity from human lung HFL1 cells may explain neurological and autoimmune manifestations of COVID-19

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Section: Resultsmentioning

confidence: 99%

An Autoantigen Atlas from Human Lung HFL1 Cells Offers Clues to Neurological and Diverse Autoimmune Manifestations of COVID-19

Wang¹,

Zhang

Mw³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Für einen schweren Verlauf einer COVID-19-Pneumonie werden zumeist als Risikofaktoren das Vorliegen einer Arteriosklerose oder Diabetes mellitus diskutiert, denen beide jeweils eine Mikro-und Makroangiopathie mit vorgeschädigtem Endothel zugrunde liegt. Nach Infektion der Alveolarepithelzellen und der Endothelzellen kommt es zur intrazellulären viralen Replikation im endoplasmatischen Retikulum [15][16][17]. Gleichermaßen unterliegen Zellbestandteile, wie z.…”

Section: Endotheliale Dysfunktion Und Angiogeneseunclassified

“…Gleichermaßen unterliegen Zellbestandteile, wie z. B. Zellmembran, Golgi-Apparat oder Mitochondrien, sowie das Zytoskelett virolytischen Veränderungen und Umbauvorgängen [15,16]. Nach mehrfacher Replikation und Ausschleusung der Viruspartikel aus der Zelle [17] kommt es zur Apoptose [15,16].…”

Section: Endotheliale Dysfunktion Und Angiogeneseunclassified

“…B. Zellmembran, Golgi-Apparat oder Mitochondrien, sowie das Zytoskelett virolytischen Veränderungen und Umbauvorgängen [ 15 , 16 ]. Nach mehrfacher Replikation und Ausschleusung der Viruspartikel aus der Zelle [ 17 ] kommt es zur Apoptose [ 15 , 16 ]. Die apoptotische Endothelzelle [ 14 , 15 ] löst sich durch Aufklaffen der engen Zellkontakten, wie z.…”

Section: Covid-19-pneumonieunclassified

“…Nach mehrfacher Replikation und Ausschleusung der Viruspartikel aus der Zelle [ 17 ] kommt es zur Apoptose [ 15 , 16 ]. Die apoptotische Endothelzelle [ 14 , 15 ] löst sich durch Aufklaffen der engen Zellkontakten, wie z. B. über „tight junctions“, Desmosomen und Hemidesmosomen, von den Nachbarzellen und der Basalmembran.…”

Section: Covid-19-pneumonieunclassified

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COVID-19: Auswirkungen auf Lunge und Herz

et al. 2021

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Autophagy as an innate immunity response against pathogens: a Tango dance

Aguilera,

Delgui,

Reggiori

et al. 2023

FEBS Letters

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Intracellular infections as well as changes in the cell nutritional environment are main events that trigger cellular stress responses. One crucial cell response to stress conditions is autophagy. During the last 30 years, several scenarios involving autophagy induction or inhibition over the course of an intracellular invasion by pathogens have been uncovered. In this review, we will present how this knowledge was gained by studying different microorganisms. We intend to discuss how the cell, via autophagy, tries to repel these attacks with the objective of destroying the intruder, but also how some pathogens have developed strategies to subvert this. These two fates can be compared with a Tango, a dance originated in Buenos Aires, Argentina, in which the partner dancers are in close connection. One of them is the leader, embracing and involving the partner, but the follower may respond escaping from the leader. This joint dance is indeed highly synchronized and controlled, perfectly reflecting the interaction between autophagy and microorganism.

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Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies

Cited by 235 publications

References 45 publications

An Autoantigen Atlas from Human Lung HFL1 Cells Offers Clues to Neurological and Diverse Autoimmune Manifestations of COVID-19

An Autoantigen Atlas from Human Lung HFL1 Cells Offers Clues to Neurological and Diverse Autoimmune Manifestations of COVID-19

COVID-19: Auswirkungen auf Lunge und Herz

Autophagy as an innate immunity response against pathogens: a Tango dance

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