Integrative Characterization of Toxic Response of Zebra Fish(Danio rerio)to Deltamethrin Based on AChE Activity and Behavior Strength

Abstract

In order to characterize the toxic response of zebra fish (Danio rerio) to Deltamethrin (DM), behavior strength (BS) and muscle AChE activity of zebra fish were investigated. The results showed that the average values of both BS and AChE activity showed a similarly decreased tendency as DM concentration increased, which confirmed the dose-effect relationship, and high and low levels of AChE and BS partly matched low and high levels of exposure concentrations in self-organizing map. These indicated that AChE and BS had slight different aspects of toxicity although overall trend was similar. Behavior activity suggested a possibility of reviving circadian rhythm in test organisms after exposure to the chemical in lower concentration (0.1 TU). This type of rhythm disappeared in higher concentrations (1.0 TU and 2.0 TU). Time series trend analysis of BS and AChE showed an evident time delayed effect of AChE, and a 2 h AChE inhibition delay with higher correlation coefficients (r) in different treatments was observed. It was confirmed that muscle AChE inhibition of zebra fish is a factor for swimming behavior change, though there was a 2 h delay, and other factors should be investigated to illustrate the detailed behavior response mechanism.

Ecotoxicity Assessment of Fe3O4 Magnetic Nanoparticle Exposure in Adult Zebrafish at an Environmental Pertinent Concentration by Behavioral and Biochemical Testing

“…AChE plays primary control in multiple physiological processes [58,59] and has been proven to impart swimming disorders in fish [59,60]. We observed a significant reduction in the amount of AChE in fish brain tissues at a high concentration of 10 ppm MNPs as well as at 1 ppm MNPs when compared to the control group ( p = 0.03).…”

The Monitoring and Assessment of Cd2+ Stress Using Zebrafish (Danio rerio)

“…Then, we checked p value to see whether these two variables are correlated significantly (p < 0.05). When r is high (absolute r > 0.5) with significance (p < 0.05), it indicates that the data correlation is significant [53,54].…”

Phototropic response features for different systematic groups of mesoplankton under adverse environmental conditions

“…Over the past two decades, quite a few studies have been devoted to water biotesting techniques based on the analysis of various features of aquatic organisms, namely survival registration (OECD, 2013(OECD, , 2019, reproducibility, offspring quality, morphological parameter changes (OECD, 2013), physiological functions, and behavioral responses (Lechelt et al, 2000;Morgalev et al, 2015;Nikitin, 2014;Wang et al, 2019). The most promising are the methods of water biomonitoring using behavioral responses of local species of hydrobionts, and primarily bivalves (Sukharenko et al, 2017), branchiopoda (Carreño-León et al, 2014) and copepoda (Lechelt et al, 2000;Pan et al, 2015Pan et al, , 2017Ren et al, 2017), and fish (Ren et al, 2016). Daphnia are particularly interesting with regard to these methods.…”