Integrative approach reveals new insights into photosynthetic and redox protection in ex vitro tobacco plantlets acclimatization to increasing light intensity

Vieira, Cinthya F.; Carvalho, Fernanda Torres de; Lima‐Melo, Yugo; Carvalho, Cristina Paiva da Silveira; Neto, Milton Costa Lima; Martins, Márcio O.; Silveira, Joaquim Albenísio Gomes da

doi:10.1016/j.biori.2020.04.001

Cited by 7 publications

(6 citation statements)

References 62 publications

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“…That is because several anatomical, morphological, and physiological characteristics of in vitro growth (e.g., reduced epicuticular waxes, malfunctional stomata, and low levels of chlorophyll) limit photosynthetic capacity and water-loss regulation during the initial phases of ex vitro acclimation (Gago et al, 2014;S aez et al, 2013). Therefore, it is generally recommended to progressively change environmental conditions such as PPFD and humidity during acclimation to reduce shrinkage or minimize growth delays of TC transplants (Chandra et al, 2010;Posp ı silov a et al, 1999;Vieira et al, 2019;Zobayed, 2020). In commercial greenhouses, this is typically achieved by controlling shade curtains, supplemental lighting, and mist irrigation, among others.…”

Section: Discussionmentioning

confidence: 99%

“…1 and 2). However, further studies are needed to identify the PPFD threshold that will maximize growth without inducing undesirable stress responses, which could include the production of reactive oxygen species in chloroplasts and the accumulation of antioxidant enzymes, among others (Van Huylenbroeck et al, 2000;Vieira et al, 2019). Accordingly, although not measured in Expt.…”

Section: Discussionmentioning

confidence: 99%

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Radiation Intensity and Quality Affect Indoor Acclimation of Blueberry Transplants

Gómez

Poudel

Yegros

et al. 2021

horts

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The objectives were to characterize and compare shrinkage (i.e., transplant loss) and growth of tissue-cultured blueberry (Vaccinium corymbosum) transplants acclimated in greenhouses or indoors under 1) different photosynthetic photon flux densities (PPFDs) (Expt. 1); or 2) spectral changes over time using broad-spectrum white (W; 400 to 700 nm) light-emitting diodes (LEDs) without or with red or far-red (FR) radiation (Expt. 2). In Expt. 1, ‘Emerald’ and ‘Snowchaser’ transplants were acclimated for 8 weeks under PPFDs of 35, 70, 105, or 140 ± 5 µmol·m‒2·s‒1 provided by W LED fixtures for 20 h·d−1. In another treatment, PPFD was increased over time by moving transplants from treatment compartments providing 70 to 140 µmol·m‒2·s‒1 at the end of week 4. Transplants were also acclimated in either a research or a commercial greenhouse (RGH or CGH, respectively). Shrinkage was unaffected by PPFD, but all transplants acclimated indoors had lower shrinkage (≤4%) than those in the greenhouse (15% and 17% in RGH and CGH, respectively), and generally produced more shoot and root biomass, regardless of PPFD. Growth responses to increasing PPFD were linear in most cases, although treatment effects after finishing were generally not significant among PPFD treatments. In Expt. 2, ‘Emerald’ transplants were acclimated for 8 weeks under constant W, W + red (WR), or W + FR (WFR) radiation, all of which provided a PPFD of 70 ± 2 μmol·m−2·s−1 for 20 h·d−1. At the end of week 4, a group of transplants from WR and WFR were moved to treatment compartments with W (WRW or WFRW, respectively) or from W to a research greenhouse (WGH), where another group of transplants were also acclimated for 8 weeks (GH). Shrinkage of transplants acclimated indoors was also low in Expt. 2, ranging from 1% to 4%. In contrast, shrinkage of transplants acclimated in GH or under WGH was 37% or 14%, respectively. Growth of indoor-acclimated transplants was generally greater than that in GH or under WGH. Although growth responses were generally similar indoors, plants acclimated under WFR had a higher root dry mass (DM) and longer roots compared with GH and WGH.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Radiation Intensity and Quality Affect Indoor Acclimation of Blueberry Transplants

Gómez

Poudel

Yegros

et al. 2021

horts

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“…In this study, the shoots obtained with all treatments achieved high survival rates irrespective of the presence or absence of roots. Survival during acclimatization is related to the plant vigor and a highly developed foliar system (Monja-Mio et al, 2015;Martínez-Estrada et al, 2019;Vieira et al, 2020). Additionally, the wax content and thickness of the cuticle in this species, coupled with the type of metabolism (CAM), allowed a high survival rate during the acclimatization process.…”

Section: B Amentioning

confidence: 99%

“…Additionally, the wax content and thickness of the cuticle in this species, coupled with the type of metabolism (CAM), allowed a high survival rate during the acclimatization process. There are also external factors regulating ex vitro survival, such as water content (irrigation), fertilization, type of substrate, and environmental conditions (light, temperature, and relative humidity, among others) (Hoang et al, 2020;Vieira et al, 2020). Moreover, the anatomy and physiology of the genus Agave, together with appropriate greenhouse management, probably contributed to the adequate development of plantlets ex vivo without affecting their survival.…”

Section: B Amentioning

confidence: 99%

Micropropagation of Agave (Agave potatorum Zucc.) through Direct Organo-genesis

Ramírez-Mosqueda¹,

Cárcamo-Corona²,

Aguilar-Jiménez³

et al. 2022

agro

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Populations of Agave potatorum Zucc. have been overexploited from their habitat for the commercial production of mezcal, a traditional Mexican liquor. For this species, micropropagation is the only method for cloning selected genotypes. The aim of this study was to establish an efficient protocol for the in-vitro propagation of A. potatorum by use of individual shoots. In the propagation stage, the interaction between different concentrations of the cytokinin benzylaminopurine (BAP: 0, 1.5, and 3.0 mg L–1)and the auxin indole acetic acid (IAA: 0, 1.5, and 3.0 mg L–1) was evaluated. For in-vitro rooting, the interaction between different auxin concentrations, namely naphthaleneacetic acid (NAA: 0, 1.5 and 3.0 mg L–1) and IAA (0, 1.5 and 3.0 mg L–1) was evaluated. In the propagation stage, the highest number of shoots was obtained with the combinations of 3.0 mg L–1 BAP + 3.0 mg L–1 IAA, 1.5 mg L–1 BAP + 3.0 mg L–1 IAA, and 3.0 mg L–1 BAP + 1.5 mg L–1 IAA, which yielded 9.87, 9.73, and 9.73 shoots per explant, respectively. In the rooting stage, the best shoot development was observed in the control treatment and when only 3.0 mg L–1 IAA was supplemented. Finally, after the rooting stage, the plantlets obtained were acclimatized and grown in the field, yielding a survival rate of 98-100%. In conclusion, this propagation protocol contributes to obtaining commercial propagules suitable for establishment in the field.

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“…Gaseous exchange between the vials atmosphere and the outside environments favors the growth and development of plant tissues (explant) during in vitro growth. It improves the plant's adaptation to the acclimatization stage since the gas exchange is directly involved in the quantity and stomatal efficiency and plants' metabolite production (Iarema et al, 2012;Mohamed and Alsadon, 2010;Taiz et al, 2017;Vieira et al, 2019). Some techniques have been used to control gas concentrations and gas exchange in vitro growth, such as caps with a microporous membrane (Mohamed and Alsadon, 2011;Santana et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Biochar as an alternative to improve the in vitro environment for Pitaya (Hylocereus undatus Haw) and strawberry (Fragaria x ananassa Duch) growing

Laura¹,

Érika²,

Claudinéia³

et al. 2023

Afr. J. Agric. Res.

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In vitro cultivation is widely used for micropropagation of several plant species; however, the increase in the gas concentration, such as ethylene gas, inside the growth vials can cause physiological changes. Thus, 4 experiments were carried out at the Federal University of Minas Gerais to evaluate the biochar use from sugarcane bagasse in vials with or without caps with porous membrane in the in vitro growing of pitaya (Hylocereus undatus Haw) (experiment 1) and strawberry (Fragaria x ananassa Duch) (experiment 2) and evaluating in combination of the use of biochar from sugarcane bagasse and AgNO 3 in the in vitro growing of pitaya (experiment 3) and strawberry (experiment 4). The types of caps did not affect the growth and number of pitaya and strawberry; however, using caps with porous membrane obtained more magnificent roots dry matter yield. The addition of 4 g L -1 of biochar, regardless of cap type, favored the pitaya and strawberry roots' growth and development. AgNO 3 alone contributed to the higher length and dry mass of pitaya roots and greater dry mass of strawberry roots. Adding biochar decreases the AgNO 3 effects in vitro culture. Biochar is an alternative to improve the in vitro environment of plant species.

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Integrative approach reveals new insights into photosynthetic and redox protection in ex vitro tobacco plantlets acclimatization to increasing light intensity

Cited by 7 publications

References 62 publications

Radiation Intensity and Quality Affect Indoor Acclimation of Blueberry Transplants

Radiation Intensity and Quality Affect Indoor Acclimation of Blueberry Transplants

Micropropagation of Agave (Agave potatorum Zucc.) through Direct Organo-genesis

Biochar as an alternative to improve the in vitro environment for Pitaya (Hylocereus undatus Haw) and strawberry (Fragaria x ananassa Duch) growing

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