Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Lv, Junheng; Deng, Minghua; Li, Zuosen; Zhu, Haishan; Wang, Ziran; Yue, Yanling; Zhou, Yang; Xu, Junqiang; Jiang, Shurui; Zhao, Wei; Li, Jing; Zhao, Kai

doi:10.1016/j.hpj.2022.12.008

Cited by 5 publications

(8 citation statements)

References 57 publications

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“…Similarly, NB-LRR genes were overexpressed in a TSWV resistant tetraploid peanut cultivar than the susceptible tetraploid cultivar following TSWV infection ( Catto et al., 2021 ). NB-LLR genes also were overexpressed in response to TSWV infection in TSWV-resistant tomato lines in another study ( Lv et al., 2023 ). The overexpression of defense genes following thrips-mediated TSWV inoculation in this study provides mechanistic reasons for the observed response against TSWV in A. valida .…”

Section: Discussionmentioning

confidence: 89%

“…WRKY transcription factors also were involved in triggering immunity against a range of pathogens including viruses by recognizing pathogen associated molecular patterns (PAMPs) ( Pandey and Somssich, 2009 ; Lee et al., 2023 ). WRKY was overexpressed in a tomato genotype with resistance to TSWV ( Catoni et al., 2009 ; Lv et al., 2023 ). Similarly, WRKY contigs were substantially overexpressed in A. valida and slightly in the hybrid.…”

Section: Discussionmentioning

confidence: 99%

“…Results in this study showed that genes related to abscisic acid (ABA) and auxin were underexpressed in wild peanut species. Likewise, the DEGs associated with auxin were underexpressed following TSWV infection in susceptible and resistant tomato lines, while DEGs related to ethylene were overexpressed ( Lv et al., 2023 ). In contrast, the miRNA associated with auxin pathways were overexpressed in pepper plants following TSWV infection ( Tao et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of gene expression patterns in response to an orthotospovirus infection between two diploid peanut species and their hybrid

Chen,

Catto,

Pandey

et al. 2023

Front. Plant Sci.

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Tomato spotted wilt orthotospovirus (TSWV) transmitted by thrips causes significant yield loss in peanut (Arachis hypogaea L.) production. Use of peanut cultivars with moderate field resistance has been critical for TSWV management. However, current TSWV resistance is often not adequate, and the availability of sources of tetraploid resistance to TSWV is very limited. Allotetraploids derived by crossing wild diploid species could help introgress alleles that confer TSWV resistance into cultivated peanut. Thrips-mediated TSWV screening identified two diploids and their allotetraploid possessing the AA, BB, and AABB genomes Arachis stenosperma V10309, Arachis valida GK30011, and [A. stenosperma × A. valida]4x (ValSten1), respectively. These genotypes had reduced TSWV infection and accumulation in comparison with peanut of pure cultivated pedigree. Transcriptomes from TSWV-infected and non-infected samples from A. stenosperma, A. valida, and ValSten1 were assembled, and differentially expressed genes (DEGs) following TSWV infection were assessed. There were 3,196, 8,380, and 1,312 significant DEGs in A. stenosperma, A. valida, and ValSten1, respectively. A higher proportion of genes decreased in expression following TSWV infection for A. stenosperma and ValSten1, whereas a higher proportion of genes increased in expression following infection in A. valida. The number of DEGs previously annotated as defense-related in relation to abiotic and biotic stress was highest in A. valida followed by ValSten1 and A. stenosperma. Plant phytohormone and photosynthesis genes also were differentially expressed in greater numbers in A. valida followed by ValSten1 and A. stenosperma, with over half of those exhibiting decreases in expression.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of gene expression patterns in response to an orthotospovirus infection between two diploid peanut species and their hybrid

Chen,

Catto,

Pandey

et al. 2023

Front. Plant Sci.

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“…Multi-omics studies play a crucial role in exploring plant-pathogen interactions. Recently, an increasing number of studies used metabolome and transcriptome association analyses to identify differential metabolites and genes ( Li et al, 2020 ; Lv et al, 2023 ). These studies provide a theoretical foundation to comprehensively investigate the molecular mechanisms underlying plant-virus interactions.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive phytohormone metabolomic and transcriptomic analysis of tobacco (Nicotiana tabacum) infected by tomato spotted wilt virus (TSWV)

Liang,

Wang,

Zhang

et al. 2024

Virus Research

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“…Both PTI and ETI induce the production of defense‐related secondary metabolites, pathogen‐associated transcription factors (TFs) and pathogenesis‐related (PR) proteins, as well as activate hormone signaling and calcium (Ca 2+ ) signaling (Chai et al, 2021). Recent studies have utilized transcriptomics and metabolomics to analyze the response mechanisms of plants such as sealwort, tomato, and Chinese prickly ash under pathogen stress (Li et al, 2021; Pang et al, 2022; Lv et al, 2022). These studies have laid a foundation for the discovery and utilization of resistance genes and biomarkers for disease prevention and treatment.…”

Section: Introductionmentioning

confidence: 99%

The endophytic fungal community plays a crucial role in the resistance of host plants to necrotic bacterial pathogens

Yang,

Gao,

Guo

et al. 2024

Physiologia Plantarum

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Konjac species (Amorphophallus spp.) are the only plant species in the world that are rich in a large amount of konjac glucomannan (KGM). These plants are widely cultivated as cash crops in tropical and subtropical countries in Asia, including China. Pectobacterium carotovorum subsp. carotovorum (Pcc) is one of the most destructive bacterial pathogens of konjac. Here, we analyzed the interactions between Pcc and susceptible and resistant konjac species from multiple perspectives. At the transcriptional and metabolic levels, the susceptible species A. konjac and resistant species A. muelleri exhibit similar molecular responses, activating plant hormone signaling pathways and metabolizing defense compounds such as phenylpropanoids and flavonoids to resist infection. Interestingly, we found that Pcc stress can lead to rapid recombination of endophytic microbial communities within a very short period (96 h). Under conditions of bacterial pathogen infection, the relative abundance of most bacterial communities in konjac tissue decreased sharply compared with that in healthy plants, while the relative abundance of some beneficial fungal communities increased significantly. The relative abundance of Cladosporium increased significantly in both kinds of infected konjac compared to that in healthy plants, and the relative abundance in resistant A. muelleri plants was greater than that in susceptible A. konjac plants. Among the isolated cultivable microorganisms, all three strains of Cladosporium strongly inhibited Pcc growth. Our results further elucidate the potential mechanism underlying konjac resistance to Pcc infection, highlighting the important role of endophytic microbial communities in resisting bacterial pathogen infections, especially the more direct role of fungal communities in inhibiting pathogen growth.

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Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Cited by 5 publications

References 57 publications

Characterization of gene expression patterns in response to an orthotospovirus infection between two diploid peanut species and their hybrid

Characterization of gene expression patterns in response to an orthotospovirus infection between two diploid peanut species and their hybrid

Comprehensive phytohormone metabolomic and transcriptomic analysis of tobacco (Nicotiana tabacum) infected by tomato spotted wilt virus (TSWV)

The endophytic fungal community plays a crucial role in the resistance of host plants to necrotic bacterial pathogens

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