Integrative analysis of the metabolome and transcriptome provides novel insights into the mechanisms of flavonoid biosynthesis in Camellia lanceoleosa

Song, Qiling; Gong, Wenfang; Yu, Xinran; Ji, Ke; Chang, Yihong; Wang, Linkai; Yuan, Deyi

doi:10.1016/j.scienta.2022.111357

Cited by 5 publications

(2 citation statements)

References 83 publications

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“…It is a rather a complex genetic network containing multiple genes. It has been reported that TFs play an important role in plant biological processes by regulating relative gene expression, including plant growth, synthesis of secondary metabolites ( Song et al., 2022 ), stress/stimulus responses ( Luo et al., 2022 ), flower senescence ( Ji et al, 2022 ), SI reaction processes ( Hou et al., 2022 ), pollen tube growth ( Zou et al., 2010 ) and other important biological processes. In tomato, progressive mutation of TFs led to a transition from outcrossing to self-pollination ( Benoit, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility in Camellia oleifera

et al. 2023

Front. Plant Sci.

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IntroductionSelf-incompatibility (SI) is an important strategy for plants to maintain abundant variation to enhance their adaptability to the environment. Camellia oleifera is one of the most important woody oil plants and is widely cultivated in China. Late acting self-incompatibility (LSI) in C. oleifera results in a relatively poor fruit yield in the natural state, and understanding of the LSI mechanism remains limited. MethodsTo better understand the molecular expression and gene coexpression network in the LSI reaction in C. oleifera, we conducted self- and cross-pollination experiments at two different flower bud developmental stages (3–4 d before flowering and 1 d before flowering), and cytological observation, fruit setting rate (FSR) investigation and RNA-Seq analysis were performed to investigate the mechanism of the male −female interaction and identify hub genes responsible for the LSI in C. oleifera.ResultsBased on the 21 ovary transcriptomes, a total of 7669 DEGs were identified after filtering out low-expression genes. Weighted gene coexpression network analysis (WGCNA) divided the DEGs into 15 modules. Genes in the blue module (1163 genes) were positively correlated with FSR, and genes in the pink module (339 genes) were negatively correlated with FSR. KEGG analysis indicated that flavonoid biosynthesis, plant MAPK signaling pathways, ubiquitin-mediated proteolysis, and plant-pathogen interaction were the crucial pathways for the LSI reaction. Fifty four transcription factors (TFs) were obtained in the two key modules, and WRKY and MYB were potentially involved in the LSI reaction in C. oleifera. Network establishment indicated that genes encoding G-type lectin S-receptor-like serine (lecRLK), isoflavone 3’-hydroxylase-like (CYP81Q32), cytochrome P450 87A3-like (CYP87A3), and probable calcium-binding protein (CML41) were the hub genes that positively responded to the LSI reaction. The other DEGs inside the two modules, including protein RALF-like 10 (RALF), F-box and pectin acetylesterase (MTERF5), might also play vital roles in the LSI reaction in C. oleifera.DiscussionOverall, our study provides a meaningful resource for gene network studies of the LSI reaction process and subsequent analyses of pollen−pistil interactions and TF roles in the LSI reaction, and it also provides new insights for exploring the mechanisms of the LSI response.

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Section: Discussionmentioning

confidence: 99%

Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility in Camellia oleifera

et al. 2023

Front. Plant Sci.

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“…Camellia oleifera, commonly known as the tea-oil tree, is a significant oil crop that is widely cultivated in China, South Korea, India, and Vietnam [1,2]. As one of the four major woody oil species in the world, C. oleifera seed oil possesses significant medicinal and nutritional value, and is rich in unsaturated fatty acids and bioactive compounds [3,4]. At present, the cultivation of elite cultivars with heterosis by hybrid breeding is an important way to increase the yield of C. oleifera.…”

Section: Introductionmentioning

confidence: 99%

Abnormal Calcium Accumulation and ROS Homeostasis-Induced Tapetal Programmed Cell Death Lead to Pollen Abortion of Petaloid-Type Cytoplasmic Male Sterility in Camellia oleifera

Gao,

Yang,

et al. 2024

Agronomy

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Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of heterosis. The petaloid anther abortion in oil tea (Camellia oleifera Abel.) constitutes a CMS phenomenon, which is of great value for the hybrid breeding of oil tea. However, as the mechanism of its CMS is still poorly understood, it is necessary to study the cytology and physiological characteristics of anther abortion. In this study, a C. oleifera cultivar, Huashuo (HS), and its petalized CMS mutant (HSP) were used as materials to explore this mechanism. Compared with HS, cytological analysis demonstrated that HSP showed early-onset tapetum programmed cell death (PCD) and an organelle disorder phenotype during the tetrad stage. In HSP, anthers exhibited elevated levels of calcium deposition in anther wall tissues, tapetum layers, and microspores, and yet calcium accumulation was abnormal at the later stage. The contents of hydrogen peroxide and MDA in HSP anthers were higher, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were lower than those of HS, which resulted in an excessive accumulation of reactive oxygen species (ROS). Real-time quantitative PCR confirmed that the transcription levels of CoPOD and CoCAT genes encoding key antioxidant enzymes in HSP were downregulated compared with HS in early pollen development; the gene CoCPK, which encodes a calcium-dependent protein kinase associated with antioxidase, was upregulated during the critical period. Thus, we suggest that excessive ROS as a signal breaks the balance of the antioxidant system, and along with an abnormal distribution of calcium ions, leads to the early initiation of PCD in the tapetum, and ultimately leads to pollen abortion for HSP. These results lay a cytological and physiological foundation for further studies on the CMS mechanism, and provide information for breeding male-sterile lines of C. oleifera.

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Polymethoxyflavone (PMF) Accumulation and PMF Biosynthesis-Related Gene Expression in Tachibana (Citrus tachibana Tanaka) Fruit Treated Using a Combination of Gibberellic Acid and Prohydrojasmon and Post-harvest Deep-Ultraviolet Light-Emitting Diode Irradiation

Yamaga,

Endo,

et al. 2023

J Plant Growth Regul

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Integrative analysis of the metabolome and transcriptome provides novel insights into the mechanisms of flavonoid biosynthesis in Camellia lanceoleosa

Cited by 5 publications

References 83 publications

Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility in Camellia oleifera

Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility in Camellia oleifera

Abnormal Calcium Accumulation and ROS Homeostasis-Induced Tapetal Programmed Cell Death Lead to Pollen Abortion of Petaloid-Type Cytoplasmic Male Sterility in Camellia oleifera

Polymethoxyflavone (PMF) Accumulation and PMF Biosynthesis-Related Gene Expression in Tachibana (Citrus tachibana Tanaka) Fruit Treated Using a Combination of Gibberellic Acid and Prohydrojasmon and Post-harvest Deep-Ultraviolet Light-Emitting Diode Irradiation

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