Integrative Analysis of the Coloring Mechanism of Red Longan Pericarp through Metabolome and Transcriptome Analyses

Yi, Debao; Zhang, Hongna; Lai, Bo; Liu, Liqin; Pan, Xiaolu; Ma, Zhengqiang; Wang, Yicheng; Xie, Jianhua; Wei, Yongzan

doi:10.1021/acs.jafc.0c05023

Cited by 76 publications

(81 citation statements)

References 46 publications

(95 reference statements)

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“…This result is similar to a study that determines the pathway by which red longan ( Dimocarpus longan ) fruits were produced. Yi et al (2021) revealed that genes related to enzymes leading up to dihydrokaempferol were significantly upregulated in red pericarp longan fruits. While the pathways to produce red pigments might be similar between these two distantly related species, the timing for activating them is quite different as the pathway is initiated in young leaves of H. hainanensis and in maturing fruits of longan.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, we found that in the anthocyanin biosynthesis of RU vs. RL, some UFGT genes (cluster-15967.49721) were negatively correlated with pelargonidin-3-o-glucoside (−0.831) as the log2Fold Change of this gene in RU vs. GU was −1.83. Downregulation of some UFGT genes was also found in red pericarp longan fruits ( Yi et al, 2021 ) and thought to be involved with increased anthocyanin accumulation. Furthermore, the high expression of FLS and the accumulation of the flavonoid apigenin (pmp000571) also revealed that flavonoids, in addition to anthocyanins, pelargonidin, and cyanidin, are also responsible for the red color of young red-leaved lines.…”

Section: Discussionmentioning

confidence: 99%

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Integrated Metabolome and Transcriptome Analyses Reveal Dissimilarities in the Anthocyanin Synthesis Pathway Between Different Developmental Leaf Color Transitions in Hopea hainanensis (Dipterocarpaceae)

et al. 2022

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Changes in plant leaf color during development are directly related to the accumulation or degradation of certain phytochemicals such as anthocyanins. Since some anthocyanins can be beneficial to human health and provide insights into the biology of leaves, the underlying processes and timing by which plants produce these molecules has been the focus of numerous studies. The tree species Hopea hainanensis generally produces green leaves at all growth stages; however, a few explored individuals have been identified possessing red leaves on the top of the seedlings at a young stage. While the phenomenon of leaf color varying with age has been studied in several species, the underlying mechanisms are largely unknown in H. hainanensis. Using a metabolomics approach, the young red leaves in H. hainanensis were found to contain higher levels of anthocyanins and flavonoids than the young green-leaved individuals. Among anthocyanins, pelargonidin and cyanidin were the most likely candidates contributing to the red color of the young leaves. Transcriptome results indicated the genes related to the production of these anthocyanins were significantly upregulated, leading to greater accumulation of red pigments. Specifically, the expression of several MYB and bHLH genes in young red leaf lines was significantly higher than that in the young green leaf lines, especially HhMYB66, HhMYB91, HhMYB6, and HhbHLH70. As such these four transcription factors are probably the main regulatory genes resulting in young red leaves in H. hainanensis. From these results, comparative analyses with other species can be made to better understand the evolution of pigment biosynthesis and how anthocyanins function in plant metabolism and evolution/adaptation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated Metabolome and Transcriptome Analyses Reveal Dissimilarities in the Anthocyanin Synthesis Pathway Between Different Developmental Leaf Color Transitions in Hopea hainanensis (Dipterocarpaceae)

et al. 2022

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“…Notably, the levels of two anthocyanins, cyanidin O -syringic acid and pelargonidin 3- O -beta-d-glucoside (callitephin chloride), increased significantly during fruit peel development in C. humilis . Besides, Yi et al [ 40 ] found that Cyanidin O -syringic acid is one of the main anthocyanin derivatives of red pericarp longan. Accordingly, we suggested that the accumulation of these anthocyanins accounted may be the reason for the red pigmentation of fruit peel during fruit development.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of Cerasus humilis Peel Coloration

Ren

Zhang

et al. 2021

IJMS

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Cerasus humilis is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolomics analysis, 16 anthocyanin components were identified at four developmental stages, and high levels of cyanidin O-syringic acid and pelargonidin 3-O-beta-d-glucoside (callitephin chloride) were correlated with the reddening of the fruit peel. A co-expression analysis revealed that ANS and UFGT play key roles in pigmentation (PCC > 0.82). Additionally, transcriptome data showed that most anthocyanin biosynthetic genes and two MYB transcription factors were significantly up-regulated. QRT-PCR results for these differentially expressed genes were generally consistent with the high-throughput sequencing. Moreover, the overexpression of ChMYB1 (TRINITY_DN21536_c0_g1) in apple calli could contribute to the accumulation of anthocyanin. It was also found that UFGT (TRINITY_DN19893_c1_g5) and ChMYB1 (TRINITY_DN21536_c0_g1) have similar expression patterns. These findings provide insight into the mechanisms underlying anthocyanin accumulation and coloration during fruit peel development, providing a basis for the breeding of anthocyanin-rich C. humilis cultivars.

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“…The expression abundance of unigene was represented as FPKM (Fragments Per Kilobase of transcript per Million fragments mapped) value, and the DEGs [false discovery rate (FDR) < 0.05, and | Log 2 FC | ≥ 1.5] between red leaves and green leaves were obtained using the DESeq 1.8.3 package [47]. Function annotation was performed using Gene Ontology (GO) and the pathways with signi cant enrichment were identi ed based on KEGG pathway employing the cluster Pro ler R package [48].…”

Section: Rna Extraction Sequencing and Transcriptome Data Analysismentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in red walnut

Wang¹,

Li²,

Zhao³

et al. 2021

Preprint

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Background Walnut is one of the most important dry fruit crops worldwide, typically green leaves and yellow-brown or gray-yellow seed coats. A specific walnut type, red walnut ‘RW-1’ with red leaves and seed coats was selected as plant material because of higher anthocyanins contents. Anthocyanins are important colorants with strong antioxidant activity, especially, benefic for human health. However, few studies focused on the molecular mechanism of anthocyanin biosynthesis in walnut. Results From the results of Widely Targeted Metabolome and anthocyanidin detection analysis, 395 substances, including 4 procyanidins and 26 anthocyanins, were identified from the red-leaf walnuts of RW-1 natural hybrid progenies (SR) and the green-leaf walnuts of RW-1 natural hybrid progenies (SG). Among these, all the anthocyanins in SR were significantly up-accumulated comparing with SG. Also, delphinidin 3-O-galactoside, cyanidin 3-O-galactoside, delphinidin 3-O-glucoside and cyanidin 3-O-glucoside were identified to the primary components of anthocyanidins because of the higher contents. It was noted that 9 anthocyanins including malvidin 3-O-galactoside, malvidin 3-O-arabinoside, cyanidin 3-O-(6-O-malonyl-beta-D-glucoside), delphinidin 3-O-glucoside, delphinidin 3,5-O-diglucoside (Delphin), peonidin 3-O-(6-O-malonyl-beta-D-glucoside), petunidin 3-O-(6-O-malonyl-beta-D-glucoside), petunidin 3-O-arabinoside and pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside) were detected only in SR walnut. Furthermore, transcriptome analysis demonstrated that the expression of structural genes (C4H, F3H, F3’5’H and UFGTs), and four MYBs in anthocyanin biosynthetic pathway were significantly higher in SR walnut. Conclusions We identified the color formation of SR leaves is due to the accumulation of anthocyanins. And our results obtained the valuable information on the anthocyanin metabolites and candidate genes in anthocyanin biosynthesis, which provided new insights into the anthocyanin biosynthesis in walnuts.

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Integrative Analysis of the Coloring Mechanism of Red Longan Pericarp through Metabolome and Transcriptome Analyses

Cited by 76 publications

References 46 publications

Integrated Metabolome and Transcriptome Analyses Reveal Dissimilarities in the Anthocyanin Synthesis Pathway Between Different Developmental Leaf Color Transitions in Hopea hainanensis (Dipterocarpaceae)

Integrated Metabolome and Transcriptome Analyses Reveal Dissimilarities in the Anthocyanin Synthesis Pathway Between Different Developmental Leaf Color Transitions in Hopea hainanensis (Dipterocarpaceae)

Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of Cerasus humilis Peel Coloration

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in red walnut

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