Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development

Abstract

Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development.

Identification and Expression Profiling of miRNAome in Goat longissimus dorsi Muscle from Prenatal Stages to a Neonatal Stage

“…For instance, Zhao et al described the differential expression of genes, like GSK3B , IKBKB , and ACVR1 , which are associated with myogenesis in both obese and lean pig breeds [13]. In addition to the myomiRs (miR-1, miR-133 and miR-206), other miRNAs, such as miR-542-3p and let-7a, are highly expressed in skeletal muscle throughout the prenatal and neonatal periods, suggesting that other miRNAs also play important roles in regulating pig myogenesis [14]. The multifaceted roles of miRNAs in muscle growth are also true for poultry, as evidenced by a comparative analysis revealing interaction of several miRNAs with the ACVR2B gene, resulting in distinct muscle development of broiler and layer chickens [16].…”

Global transcriptional profiling of longissimus thoracis muscle tissue in fetal and juvenile domestic goat using RNA sequencing

“…Over the past few years, there has been substantial progress in understanding of the physiological processes and molecular mechanism underlying skeletal muscle growth and development in mammals (Qin et al 2013;Zhang et al 2013), including production animals (Bismuth & Relaix 2010;Du et al 2010), but the majority of studies examined the expression and function(s) of single genes, such as those in the MRF and MEF2 families (Berkes & Tapscott 2005;Ishibashi et al 2005), STIM1 (Kiviluoto et al 2011) andDNAJA1 (Cassar-Malek et al 2011). In addition, the majority of the previous studies examined single developmental pathways.…”

Role of microRNA-101a in the regulation of goat skeletal muscle satellite cell proliferation and differentiation

“…However, recently miR-101a has been detected via Solexa sequencing in skeletal muscle of mammals (Qin et al, 2013), and the expression level of mature miR-101a gradually increased during the induction time from 1 d to 5 d after the medium of C2C12 cells was changed to DM (Li et al, 2012). This expression model of miR-101a was similar with the expression profile of many miRNAs which play importantly regulatory role in myogenesis, such as miR-24 , miR-26a (Wong and Tellam, 2008), miR-206, miR-378 and miR-486 .…”