Integrative analysis of OIP5‐AS1/HUR1 to discover new potential biomarkers and therapeutic targets in multiple sclerosis

Abstract

Multiple sclerosis (MS) is a devastating autoimmune disease of the central nervous system associated with demyelination and axonal injury. This study was designed to find potential lncRNAs and their targets that are associated with the molecular basis of MS pathogenesis. In this study, peripheral blood samples were obtained from 50 relapsing‐remitting MS (RR‐MS) patients and 50 healthy controls. lncRNAs and their target were selected for validation using TaqMan Real‐Time PCR. Interactions were studied based on approaches that used to investigation biological functions and signaling pathways affected by differentially expressed messenger RNAs (mRNAs). The results of this study indicate an increase in the expression of HUR1 (p = 0.0001), CPSF7 (p = 0.02), and reduction of CSTF2 expression (p = 0.04). Also, an increase in the expression of OIP5‐AS1 (p = 0.01) was observed in men less than 30 years old. We performed a comparative analysis of the long noncoding RNAs (lncRNAs), and then we ranked them as candidate biomarkers according to a decreasing area under the receiver operating characteristic (ROC) curve (AUC) and plotted the results. Dysregulation of lncRNA expression has been linked to diseases. Further studies on the HUR1 gene can be used as diagnostic tools for the identification of high‐risk individuals in families with a history of disease before, during, and even after treatment. Our data uncovered the expression profiles of lncRNAs and mRNAs in MS patients, which will help delineate the molecular mechanisms in MS pathogenesis. However, further studies need to determine the precise role of these genes in the pathological process in MS.

Serum Biomarkers for Inflammatory Bowel Disease

“…LncRNAs have been proven to play important roles in IBD pathogenesis, including regulation of the intestinal epithelial barrier, cell apoptosis, and various immune system processes (138,139). There is growing evidence that lncRNAs may be promising diagnostic markers of various cancers, cardiovascular disease, and autoimmune disorders because they are relatively stable and simple to detect (140)(141)(142). Chen et al evaluated circulating lncRNAs levels of CD patients using microarray screening and qRT-PCR.…”

Long Non-Coding RNAs, Novel Offenders or Guardians in Multiple Sclerosis: A Scoping Review

“…Upregulation of AFAP1-AS1 promotes metastasis via modulation actin filament integrity ( 124 ). Due to its antiapoptotic properties in peripheral immune cells, it might be involved in the pathogenesis of MS ( 40 ).…”

“…GAS8-AS1 has been downregulated, while AFAP-AS1 has been upregulated in MS patients. Regarding the role of AFAP1-AS1 in the pathogenesis and progression of MS, it can be hypothesized that dysregulation of GAS8-AS1 might be involved in the pathogenesis of MS (40,125). Zha et al revealed that GAS8-AS1 negatively regulated the expression of UCA1.…”

Identification of lncRNA–miRNA–mRNA regulatory network associated with primary open angle glaucoma

“…Thus far, among these hub lncRNAs, DNAJC27-AS1, AF121898, and SNX29P2 have not been reported in any study. OIP5-AS1 is an antisense lncRNA that has been reported to play a critical role in various disorders, including oral squamous cell carcinoma [40], gastric cancer [41], cardiovascular disease [42], and multiple sclerosis [43]. Li and colleague's study revealed that OIP5-AS1 could promote the progression of oral squamous cell carcinoma by regulating the miR-338-3p/NRP1 axis [40].…”