Integrative analysis of miRNAs-mRNAs reveals that miR-182 up-regulation contributes to proliferation and invasion of nasopharyngeal carcinoma by targeting PTEN

Shi, Zhongqi; Wang, Rushi; Huang, Li-Gui; Chen, Xiao; Xu, Min; Zha, Dingjun; Ma, Yue

doi:10.18632/aging.103316

Cited by 6 publications

(6 citation statements)

References 24 publications

(32 reference statements)

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“…The dual-luciferase reporter gene assay confirmed that miR-107 decreased the transcription of TPM3-WT, while the fluorescence intensity did not change significantly in the plasmid containing TPM3-MUT. This confirmed the predicted binding area in 3'UTR (Figure 5B), which is a classical kind of regulating pattern between miRNAs and mRNAs (27,28). Furthermore, the experiments revealed that miR-107 mimics limited the proliferation, invasion, and migration, but facilitated the apoptosis of ESCC cell lines.…”

Section: Mir-107 Plays An Anti-cancer Role In Escc By Inhibiting Tpm3...supporting

confidence: 81%

MiR-107 inhibits the malignant biological behavior of esophageal squamous cell carcinoma by targeting TPM3

Zhang¹,

Zhang²,

Jiang³

et al. 2022

J Gastrointest Oncol

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Background: Esophageal squamous cell carcinoma (ESCC) is a highly lethal and aggressive tumor. Our previous study revealed that tropomyosin 3 (TPM3) is up-regulated in the late stage of ESCC and promotes epithelial-mesenchymal transition (EMT) via MMP2/MMP9. However, we have not yet explored the upstream regulator of TPM3. In this study, miR-107, a microRNA molecule, was predicted as an inhibitor targeting TPM3, and in vivo and in vitro experiments confirmed this hypothesis. Methods: Western blot and fluorescence quantitative polymerase chain reaction (qPCR) were applied to analyze the expression of miR-107 and TPM3. Flow cytometry, cell counting kit-8 (CCK8) assay, woundhealing assay, colony formation assay, transwell assay, and a BALB/c nude mouse (male, 8 weeks old, 20±2 g) model were used to detect the function of miR-107 and TPM3 in ESCC. Dual-luciferase assay was used to analyze the suppressed TPM3 expression induced by miR-107. Rescue experiments were also conducted in our research. Results:The cell and nude mouse models verified that TPM3 promotes proliferation, invasion and metastasis, and inhibits apoptosis, which is the opposite effect of miR-107 in ESCC. Meanwhile, the expression of TPM3 was up-regulated in the ESCC sample and cell lines, and miR-107 was down-regulated correspondingly. Dual-luciferase detection confirmed that miR-107 decreased the expression of TPM3 by targeting the 3'-untranslated region (3'-UTR) at the end of the TPM3 transcript. Further experiments verified that TPM3 could rescue the tumor suppression effect derived from miR-107.Conclusions: MiR-107 negatively regulates TPM3 expression and plays a tumor suppression role in ESCC.

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Section: Mir-107 Plays An Anti-cancer Role In Escc By Inhibiting Tpm3...supporting

confidence: 81%

MiR-107 inhibits the malignant biological behavior of esophageal squamous cell carcinoma by targeting TPM3

Zhang¹,

Zhang²,

Jiang³

et al. 2022

J Gastrointest Oncol

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“…However, luciferase assay results showed that miR‐182 did not target Mob1 (Figure 4I), suggesting that it may regulate activation of the HIPPO pathway in other ways. PTEN has been found to be a direct target of miR‐182 35 . At the same time, this study also found that miR‐182 and PTEN expression showed a significant negative correlation (Figure 4H).…”

Section: Discussionsupporting

confidence: 68%

“…PTEN has been found to be a direct target of miR-182. 35 At the same time, this study also found that miR-182 and PTEN expression showed a significant negative correlation (Figure 4H). Therefore, we hypothesized that miR-182 may indirectly regulate the activation of HIPPO pathway by targeting PTEN/AKT pathway.…”

Section: Discussionsupporting

confidence: 60%

Indirect regulation of HIPPO pathway by miRNA mediates high‐intensity intermittent exercise to ameliorate aging skeletal muscle function

Wang

et al. 2023

Scandinavian Med Sci Sports

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Exercise‐induced microRNA (miRNA) and HIPPO pathways participate in the regulation of skeletal muscle plasticity but their underlying mechanisms remain unclear. We aimed to investigate the effect of high‐intensity interval training (HIIT) on miRNA expression and the HIPPO pathway in the skeletal muscle of aging rats to determine its role in the amelioration of muscle aging. Thirty‐six 18‐month‐old female rats were randomly divided into sedentary control (SED, n = 12), moderate‐intensity continuous training (MICT, n = 12), and HIIT (n = 12) groups, with continuous exercise for 8 months. Quantitative reverse transcription‐polymerase chain reaction, immunoblotting, KEGG enrichment, and dual‐luciferase assays were performed on the target skeletal muscle. Compared with the SED group, the MICT and HIIT groups showed a significant trend of improvement in Lee's index and grip strength and a marked increase in skeletal muscle mitochondrial function, apoptosis, antioxidant, and lipolysis‐related protein expression. They also exhibited PI3K/AKT pathway activation and a decrease in expression of HIPPO pathway‐related proteins; 20 miRNAs were differentially expressed and enriched in the exercise group compared with the SED group, including the HIPPO pathway and metabolic pathways. Further analysis of L6 cells confirmed that miR‐182 may target PTEN, which indirectly regulates HIPPO signaling, but not Mob1. the combined application of HIIT and MICT increased the antioxidant and lipolytic capacities of skeletal muscle and improved atrophy of aging skeletal muscle; HIIT was more effective than MICT. This may be related to HIIT‐mediated AKT pathway activation and HIPPO pathway inhibition by miRNAs (miR‐486 and miR‐182).

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“…In breast cancer, it was shown that the expression of miR-182-3p was significantly increased compared to a healthy individual; the increased onco-microRNA-182-3p inhibited T cells [ 10 ]. Additionally, Shi et al (2020) [ 12 ] demonstrated that the proliferation and invasion of nasopharyngeal cancer cells were significantly increased in cells with miR-182-3p overexpression. However, in osteosarcoma (OS), the low expression of miR-182-3p in OS cell lines compared to hMSC was observed.…”

Section: Discussionmentioning

confidence: 99%

Identification of Mir-182-3p/FLI-1 Axis as a Key Signaling in Immune Response in Cervical Cancer: A Comprehensive Bioinformatic Analysis

Salmerón-Bárcenas

Mendoza-Catalán

Ramírez-Bautista

et al. 2023

IJMS

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miRNAs modulate gene expression and play critical functions as oncomiRs or tumor suppressors. The miR-182-3p is important in chemoresistance and cancer progression in breast, lung, osteosarcoma, and ovarian cancer. However, the role of miR-182-3p in cervical cancer (CC) has not been elucidated. Aim: To analyze the role of miR-182-3p in CC through a comprehensive bioinformatic analysis. Methods: Gene Expression Omnibus (GEO) databases were used for the expression analysis. The mRNA targets of miR-182-3p were identified using miRDB, TargetScanHuman, and miRPathDB. The prediction of island CpG was performed using the MethPrimer program. The transcription factor binding sites in the FLI-1 promoter were identified using ConSite+, Alibaba2, and ALGGEN-PROMO. The protein–protein interaction (PPI) analysis was performed in STRING 11.5. Results: miR-182-3p was significantly overexpressed in CC patients and has potential as a diagnostic. We identified 330 targets of miR-182-3p including FLI-1, which downregulates its expression in CC. Additionally, the aberrant methylation of the FLI-1 promoter and Ap2a transcription factor could be involved in downregulating FLI1 expression. Finally, we found that FLI-1 is a possible key gene in the immune response in CC. Conclusions: The miR-182-3p/FLI-1 axis plays a critical role in immune response in CC.

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Integrative analysis of miRNAs-mRNAs reveals that miR-182 up-regulation contributes to proliferation and invasion of nasopharyngeal carcinoma by targeting PTEN

Cited by 6 publications

References 24 publications

MiR-107 inhibits the malignant biological behavior of esophageal squamous cell carcinoma by targeting TPM3

MiR-107 inhibits the malignant biological behavior of esophageal squamous cell carcinoma by targeting TPM3

Indirect regulation of HIPPO pathway by miRNA mediates high‐intensity intermittent exercise to ameliorate aging skeletal muscle function

Identification of Mir-182-3p/FLI-1 Axis as a Key Signaling in Immune Response in Cervical Cancer: A Comprehensive Bioinformatic Analysis

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