Integrative analysis of genes and miRNA alterations in human embryonic stem cells-derived neural cells after exposure to silver nanoparticles

Oh, Jung‐Hwa; Son, Mi‐Young; Choi, Mi-Sun; Kim, Soojin; Choi, Ae Jin; Lee, Hyang‐Ae; Kim, Ki‐Suk; Kim, Jang Hwan; Song, Chang Woo; Yoon, Seokjoo

doi:10.1016/j.taap.2015.11.004

Cited by 62 publications

(51 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oxidative stress, results from an imbalance between the occurrence of reactive oxygen species (ROS) and the antioxidant defense system, has been identified as a major mechanism of nanoparticles-induced toxicity Driessen et al, 2015;Liu et al, 2015;Oh et al, 2016;Pisoschi and Pop, 2015). The so called oxidative stress always indicated by increased ROS could induce various cellular responses including several antioxidant responses (Xia et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Development of HSPA1A promoter-driven luciferase reporter gene assays in human cells for assessing the oxidative damage induced by silver nanoparticles

Xin

Wang

Zhang

et al. 2016

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Development of HSPA1A promoter-driven luciferase reporter gene assays in human cells for assessing the oxidative damage induced by silver nanoparticles

Xin

Wang

Zhang

et al. 2016

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

“…In vitro and in vivo studies also suggest that AgNPs can induce transcriptomic and non-coding RNA changes in neuronal cells and various brain regions, especially the hippocampus [12][13][14][15][16][17][18][19][20] . Behavioral disruptions, especially in cognitive functions, potential anxiogenic effects, depression-like behaviors, and altered activity levels, have been reported after direct or developmental exposure of rodents and zebrafish to AgNPs 12,13,[21][22][23][24][25] .…”

mentioning

confidence: 99%

Gut Dysbiosis and Neurobehavioral Alterations in Rats Exposed to Silver Nanoparticles

Javurek

Suresh

Spollen

et al. 2017

Sci Rep

100

View full text Add to dashboard Cite

Due to their antimicrobial properties, silver nanoparticles (AgNPs) are being used in non-edible and edible consumer products. It is not clear though if exposure to these chemicals can exert toxic effects on the host and gut microbiome. Conflicting studies have been reported on whether AgNPs result in gut dysbiosis and other changes within the host. We sought to examine whether exposure of Sprague-Dawley male rats for two weeks to different shapes of AgNPs, cube (AgNC) and sphere (AgNS) affects gut microbiota, select behaviors, and induces histopathological changes in the gastrointestinal system and brain. In the elevated plus maze (EPM), AgNS-exposed rats showed greater number of entries into closed arms and center compared to controls and those exposed to AgNC. AgNS and AgNC treated groups had select reductions in gut microbiota relative to controls. Clostridium spp., Bacteroides uniformis, Christensenellaceae, and Coprococcus eutactus were decreased in AgNC exposed group, whereas, Oscillospira spp., Dehalobacterium spp., Peptococcaeceae, Corynebacterium spp., Aggregatibacter pneumotropica were reduced in AgNS exposed group. Bacterial reductions correlated with select behavioral changes measured in the EPM. No significant histopathological changes were evident in the gastrointestinal system or brain. Findings suggest short-term exposure to AgNS or AgNC can lead to behavioral and gut microbiome changes.

show abstract

“…However, they suffer from low tissue availability and unrepresentative cellular responses, respectively. A number of recent studies have therefore used human ESC‐derived neural cell models to explore the toxicity of nanoparticles and have demonstrated that stem cells provide an excellent platform for studying the cytotoxicity of nanomaterials (Begum, Aguilar, Elias, & Hong, ; Oh et al, ; Repar et al, ). We were therefore motivated to test the utility of ESC‐derived RPE cells to evaluate the cytotoxicity of GO nanosheets and to compare their responses to two commonly used models: primary hRPEs and ARPE19 cell lines.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the cytotoxicity of graphene oxide using embryonic stem cells‐derived cells

Fu²,

Rong

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

Graphene oxide (GO) has several potential biomedical applications and therefore cytotoxic evaluation of GO is very important. However, the two most common in vitro models for testing cytotoxicity—primary human cells and immortalized cell lines—suffer serious limitations, namely limited supplies of cells and unrealistic cellular responses, respectively. Here, we demonstrate the use of embryonic stem cell (ESC)‐derived cells to study GO cytotoxicity. We tested the use of retinal pigment epithelium (RPE) cells derived from three‐dimensional human ESC cultures (“ESC‐RPE” cells) as a model of GO cytotoxicity by exposing them to varying concentrations of GO nanosheets. For comparison, we also performed the same test with primary human retinal pigment epithelium cells (“hRPE”), and with cells derived from a human RPE cell line (“ARPE19” cells). We found that cytotoxicity metrics (viability, apoptosis, intracellular reactive oxygen species, and mitochondrial membrane potential) were very similar in ESC‐RPE cells and hRPE cells, and those in ARPE19 cells were very different. We conclude that cell models of GO cytotoxicity derived from ESCs are an excellent alternative to primary human cells, without the limitations of tissue availability.

show abstract

Integrative analysis of genes and miRNA alterations in human embryonic stem cells-derived neural cells after exposure to silver nanoparticles

Cited by 62 publications

References 78 publications

Development of HSPA1A promoter-driven luciferase reporter gene assays in human cells for assessing the oxidative damage induced by silver nanoparticles

Development of HSPA1A promoter-driven luciferase reporter gene assays in human cells for assessing the oxidative damage induced by silver nanoparticles

Gut Dysbiosis and Neurobehavioral Alterations in Rats Exposed to Silver Nanoparticles

Evaluating the cytotoxicity of graphene oxide using embryonic stem cells‐derived cells

Contact Info

Product

Resources

About