Integrative analysis of DNA phylogeography and morphology of the European rose chafer (Cetonia aurata) to infer species taxonomy and patterns of postglacial colonisation in Europe

Ahrens, Dirk; Fabrizi, Silvia; Šípek, Petr; Lago, Paul K.

doi:10.1016/j.ympev.2013.05.016

Cited by 26 publications

(24 citation statements)

References 62 publications

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“…While the first scenario is well supported by the wide congruence of the DNA-based species delimitation methods and by the geographical distribution of the clades, the other two scenarios (ii, iii) cannot be entirely rejected due to the absence of clear morphological characters supporting or separating the clades. The average pairwise genetic distances of Cox1 between these three clades (4–6) are well above the interspecific divergence range published for pleurostict Scarabaeoidea [ 52 , 72 – 75 ], but see [ 13 ]. The distances within subclades of clade 6 range from 1.27 to 4.12% and correspond largely with the consensually accepted sub-specific values.…”

Section: Discussionsupporting

confidence: 56%

“…These results were corroborated later by Zauli et al [ 76 ]. The phylogeographic analysis of the European rose chafer ( Cetonia aurata ) [ 13 ] showed incongruent results from nuclear, mitochondrial, and morphological datasets. The average pairwise genetic distance between the endemic Sicilian subspecies Cetonia aurata sicula Aliquó, 1983 and populations of Cetonia aurata pisana Heer, 1841 from the Apennine peninsula was 2.1% using the mtDNA marker Cox1 .…”

Section: Discussionmentioning

confidence: 99%

“…This type of integrated approach has proven to be useful for understanding species variability and genetic diversity in related taxa (e.g. [ 13 ]). Additionally, we tested (i) the alleged species status of P .…”

Section: Introductionmentioning

confidence: 99%

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Phylogeography and DNA-based species delimitation provide insight into the taxonomy of the polymorphic rose chafer Protaetia (Potosia) cuprea species complex (Coleoptera: Scarabaeidae: Cetoniinae) in the Western Palearctic

Vondráček¹,

Fuchsová²,

Ahrens³

et al. 2018

PLoS ONE

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The development of modern methods of species delimitation, unified under the “integrated taxonomy” approach, allows a critical examination and re-evaluation of complex taxonomic groups. The rose chafer Protaetia (Potosia) cuprea is a highly polymorphic species group with a large distribution range. Despite its overall commonness, its taxonomy is unclear and subject to conflicting hypotheses, most of which largely fail to account for its evolutionary history. Based on the sequences of two mitochondrial markers from 65 individuals collected across the species range, and a detailed analysis of morphological characters including a geometric morphometry approach, we infer the evolutionary history and phylogeography of the P. cuprea species complex. Our results demonstrate the existence of three separate lineages in the Western Palearctic region, presumably with a species status. However, these lineages are in conflict with current taxonomic concepts. None of the 29 analyzed morphological characters commonly used in the taxonomy of this group proved to be unambiguously species- or subspecies- specific. The geometric morphometry analysis reveals a large overlap in the shape of the analyzed structures (pronotum, meso-metaventral projection, elytra and aedeagus), failing to identify either the genetically detected clades or the classical species entities. Our results question the monophyly of P. cuprea in regard to P. cuprina, as well as the species status of P. metallica. On the other hand, we found support for the species status of the Sicilian P. hypocrita. Collectively, our findings provide a new and original insight into the taxonomy and phylogeny of the P. cuprea species complex. At the same time, the results represent the first attempt to elucidate the phylogeography of these polymorphic beetles.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

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Phylogeography and DNA-based species delimitation provide insight into the taxonomy of the polymorphic rose chafer Protaetia (Potosia) cuprea species complex (Coleoptera: Scarabaeidae: Cetoniinae) in the Western Palearctic

Vondráček¹,

Fuchsová²,

Ahrens³

et al. 2018

PLoS ONE

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“…For example, barcoding studies of German ground beetles (Carabidae) showed intraspecific distances of up to 3.8% in the COI gene, weakly overlapping with interspecific distances which showed a minimum 3% p-distance [ 39 ]. Other Europe-wide scale studies focusing on rose beetles found intraspecific distances of up to 9% [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Local Hotspots of Endemism or Artifacts of Incorrect Taxonomy? The Status of Microendemic Pill Millipede Species of the Genus Glomeris in Northern Italy (Diplopoda, Glomerida)

Wesener‍

Conrad

2016

PLoS ONE

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Local endemic species with their unique evolutionary history always stirred the interest of scientists. One such area especially rich in endemics is northern Italy. In case of pill millipedes of the genus Glomeris Latreille, 1803, only a single species is found in northern Europe, while 22 country-endemics alone are known from Italy. Many of these endemics, however, have not been studied in several decades; therefore we aimed to determine whether this diversity is the result of overlooked synonymies or natural processes. A focus was placed on the local endemics that are in some aspects morphologically similar to the widespread and variable G. klugii Brandt, 1833. The local endemics Glomeris larii Verhoeff, 1921, G. primordialis Verhoeff, 1930, G. oblongoguttata Verhoeff, 1894, G. oropensis Verhoeff, 1936, G. transalpina Koch, 1836, G. romana Verhoeff, 1900, G. ligurica Latzel, 1884 and G. apuana Verhoeff, 1911 were included in a molecular analysis incorporating ribosomal nuclear (28S) and mitochondrial (COI) genes. Individuals were sequenced and compared to 31 specimens from 18 localities of G. klugii. The final dataset included 657 base pairs for 56 terminals in the COI, and 14 terminals with 1068 base pairs in the combined 28S and COI analysis. Our analysis shows intraspecific distances of up to 5% in the COI gene in G. klugii that are not strictly correlated to geography or color pattern. G. larii is discovered to be genetically and morphologically identical to G. klugii and is synonymised with the latter. Interspecific distances in our dataset vary between 6.7 to 15.9%, with the lowest (6.7–9.0%) between G. primordialis and G. klugii. Our analysis confirms the species status of the local endemics G. primordialis, G. oblongoguttata, G. oropensis, G. transalpina, G. ligurica and G. apuana. We also confirm the synonymy of G. undulata Koch, 1844 under G. klugii. G. genuensis Latzel, 1886 is indistinguishable from G. ligurica.

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“…However, this evidence is not strong enough to propose specific rank for each of them. A recent study showed that the high genetic divergence of COI alone cannot be a reason for species separation in Cetonia aurata aurata (Ahrens et al 2013). There is a need for additional analyses with representative sample sizes and the use of multiple genetic loci to reconfirm our results.…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of the genus Dicronocephalus (Coleoptera, Scarabaeidae, Cetoniinae) based on mtCOI and 16S rRNA genes

Lee¹,

Han²,

Jeong³

et al. 2015

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The seven species belonging to the genus Dicronocephalus are a very interesting group with a unique appearance and distinct sexual dimorphism. Only one species among them, Dicronocephalus adamsi, has been known in the Korean fauna. This species is recognized as having a wide distribution from Tibet to Korean Peninsula and is currently represented by two subspecies that have separated geographical ranges. The phylogenetic relationships of Dicronocephalus adamsi were still unclear. The phylogeny of Dicronocephalus is reconstructed with a phylogenetic study of five species including four subspecies based on a molecular approach using mitochondrial COI and 16S rRNA genes. Our results are compared with the results obtained by previous authors based on morphological characters. They show that the tested taxa are divided into two major clades. Clade A consists of two species (Dicronocephalus adamsi + Dicranocephalus yui) and Clade B includes the others (Dicronocephalus dabryi + Dicranocephalus uenoi + Dicranocephalus wallichii). This result generally supports Kurosawa’s proposal except that Dicronocephalus dabryi and Dicranocephalus uenoi are newly recognized as members of a monophyletic group. We propose that Dicronocephalus adamsi drumonti is a junior subjective synonym of Dicronocephalus adamsi adamsi. These results show that three members of the Dicranocephalus wallichii group should be treated as species rather than subspecies. However, further research including analyses of different genetic markers is needed to reconfirm our results.

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Integrative analysis of DNA phylogeography and morphology of the European rose chafer (Cetonia aurata) to infer species taxonomy and patterns of postglacial colonisation in Europe

Cited by 26 publications

References 62 publications

Phylogeography and DNA-based species delimitation provide insight into the taxonomy of the polymorphic rose chafer Protaetia (Potosia) cuprea species complex (Coleoptera: Scarabaeidae: Cetoniinae) in the Western Palearctic

Phylogeography and DNA-based species delimitation provide insight into the taxonomy of the polymorphic rose chafer Protaetia (Potosia) cuprea species complex (Coleoptera: Scarabaeidae: Cetoniinae) in the Western Palearctic

Local Hotspots of Endemism or Artifacts of Incorrect Taxonomy? The Status of Microendemic Pill Millipede Species of the Genus Glomeris in Northern Italy (Diplopoda, Glomerida)

Molecular phylogeny of the genus Dicronocephalus (Coleoptera, Scarabaeidae, Cetoniinae) based on mtCOI and 16S rRNA genes

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