Integration Traditional Chinese Medicine and Nibble Debridement and Dressing Method Reduce Thrombosis and Inflammatory Response in the Treatment of Thromboangiitis Obliterans

Abstract

Background: Thromboangiitis obliterans (TAO), also known as Buerger's disease, is an occlusive arterial disease. However, the pathogenesis of TAO is still unclear. Research has shown that traditional Chinese medicine (TCM) has significant advantages in the treatment of TAO. Our purpose is to explore the underlying roles of TCM in combination with nibble debridement and dressing method (NDDM) in TAO rat model.Methods: 10 mg/ml sodium laurate was utilized to establish a TAO rat model, and then the TAO mode rats were treated with notoginseng powder (NP), maifusheng (MFS) or the combination of NP or MFS and NDDM. Ganrene’s classification and, blood rheology was evaluated; the pathological characteristics of rat limbs were examined by H&E staining and Masson staining; CD3+ and CD20+ levels were measured by immunohistochemistry and flow cytometry. In addition, inflammation-associated cytokines were analyzed by RT-qPCR, Western blot and ELISA assays. Results: ntegration NP or MFS and NDDM dramatically reduced the ganrene’s classification and affected blood rheology parameter of TAO model rats compared with NP and MFS alone. Meanwhile, NP or MFS in combination with NDDM decreased CD3+CD20+T cells, reduced thrombosis and inflammatory cell infiltration, and dramatically decreased the levels of inflammation-associated cytokines.Conclusion: Our results suggested that integration NP or MFS and NDDM could relieve the symptoms of TAO model rats induced by sodium laurate, which might provide new management strategy for TAO.