Integration potential of mouse and human bone marrow-derived mesenchymal stem cells

Kuzma‐Kuzniarska, Maria; Rak-Raszewska, Aleksandra; Kenny, Simon E.; Edgar, David; Wilm, Bettina; Mora, Cristina; Davies, Jamie A.; Murray, Patricia

doi:10.1016/j.diff.2011.11.004

Cited by 19 publications

(25 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…Using the same chimeric rudiment culture assay, we have recently shown that mouse and human bone marrow-derived mesenchymal stem cells (MSCs) were unable to integrate into developing renal structures, and similar to Bra - cells, had a negative effect on rudiment development 43 . Conditioned medium derived from the MSCs had a similar negative effect, 43 suggesting that secreted molecules were likely to be responsible. A previous study has shown that human bone marrow-derived CD34 + cells, which consist mainly of hematopoietic stem cells, were also unable to generate renal structures in both kidney rudiments and damaged adult kidneys, and instead contributed to hematopoietic lineages, and to a lesser extent, endothelial lineages 44 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of embryonic stem cells in recombinant kidneys

et al. 2012

Self Cite

View full text Add to dashboard Cite

Embryonic stem cells (ESC) are self-renewing and can generate all cell types during normal development. Previous studies have begun to explore fates of ESCs and their mesodermal derivatives after injection into explanted intact metanephric kidneys and neonatal kidneys maturing in vivo. Here, we exploited a recently described recombinant organ culture model, mixing fluorescent quantum dot labeled mouse exogenous cells with host metanephric cells. We compared abilities of undifferentiated ESCs with ESC-derived mesodermal or non-mesodermal cells to contribute to tissue compartments within recombinant, chimeric metanephroi. ESC-derived mesodermal cells downregulated Oct4, a marker of undifferentiated cells, and, as assessed by locations of quantum dots, contributed to Wilms’ tumor 1-expressing forming nephrons, synaptopodin-expressing glomeruli, and organic ion-transporting tubular epithelia. Similar results were observed when labeled native metanephric cells were recombined with host cells. In striking contrast, non-mesodermal ESC-derived cells strongly inhibited growth of embryonic kidneys, while undifferentiated ESCs predominantly formed Oct4 expressing colonies between forming nephrons and glomeruli. These findings clarify the conclusion that ESC-derived mesodermal cells have functional nephrogenic potential, supporting the idea that they could potentially replace damaged epithelia in diseased kidneys. On the other hand, undifferentiated ESCs and non-mesodermal precursors derived from ESCs would appear to be less suitable materials for use in kidney cell therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of embryonic stem cells in recombinant kidneys

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cells were fixed using 4% (w/v) paraformaldehyde, and immunofluorescence and microscopy were performed as previously described [35]. The primary antibodies used were as follows: anti-mouse/human type II collagen antibody (CIICI; Hybridoma bank, NIH), anti-mouse osteocalcin (OG1; Santa Cruz) and anti-human osteocalcin (Osteocalcin; Santa Cruz).…”

Section: Methodsmentioning

confidence: 99%

Induction of mesenchymal stem cell chondrogenesis by polyacrylate substrates

et al. 2013

Self Cite

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) can generate chondrocytes in vitro, but typically need to be cultured as aggregates in the presence of transforming growth factor beta (TGF-β), which makes scale-up difficult. Here we investigated if polyacrylate substrates modelled on the functional group composition and distribution of the Arg-Gly-Asp (RGD) integrin-binding site could induce MSCs to undergo chondrogenesis in the absence of exogenous TGF-β. Within a few days of culture on the biomimetic polyacrylates, both mouse and human MSCs, and a mesenchymal-like mouse-kidney-derived stem cell line, began to form multi-layered aggregates and started to express the chondrocyte-specific markers, Sox9, collagen II and aggrecan. Moreover, collagen II tended to be expressed in the centre of the aggregates, similarly to developing limb buds in vivo. Surface analysis of the substrates indicated that those with the highest surface amine content were most effective at promoting MSC chondrogenesis. These results highlight the importance of surface group functionality and the distribution of those groups in the design of substrates to induce MSC chondrogenesis.

show abstract

“…Many signaling pathways have been reported to be dysregulated in PKD kidneys, and there is recent evidence indicating that metabolic reprogramming plays a critical role in ADPKD kidneys. 4,5,[7][8][9] This has resulted in preclinical studies targeting metabolic pathways, such as glycolysis and the energy sensor AMP-activated protein kinase. 7,8,10 Warner et al 1 now show that reducing food intake to levels that do not cause malnutrition (30%-50%) achieves an impressive improvement in the progression of PKD in animal models.…”

Section: Alessandra Bolettamentioning

confidence: 99%

“…These chimeric rudiments could be cultured in vitro, presenting excellent test systems for assessing whether the exogenous stem cells are capable generating specialized renal cells. 6 Using this assay, it has been shown that certain types of stem cells, most notably pluripotent stem cells 7 and amniotic fluid stem cells, 8 are capable of integrating into developing renal structures and generating specialized renal cells, whereas other cell types, such as mesenchymal stem/stromal cells, are unable to do so, 9 unless they have been engineered to overexpress glial cell line-derived neurotrophic factor (GDNF). 10 The chimeric kidney rudiment assay, therefore, allows researchers to identify stem cell types with nephrogenic potential that could be used for applications in regenerative medicine, drug discovery, and disease modeling.…”

mentioning

confidence: 99%