Integration Patterns of Cryopreserved Amniotic Membranes into the Human Cornea

Resch, Miklós; Schlötzer‐Schrehardt, Ursula; Hofmann-Rummelt, Carmen; Sauer, Roland; Kruse, Friedrich E.; Beckmann, Matthias W.; Seitz, Berthold

doi:10.1016/j.ophtha.2006.03.065

Cited by 57 publications

(44 citation statements)

References 31 publications

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“…Moreover, integration pattern of AM after applying the graft technique result in major visual limitation, if located in the pupillary region. 31 In this study, it was found that defects limited only to the centre of the graft had a better surgical success rate. This might be due to the size of the lesions.…”

Section: Discussionmentioning

confidence: 61%

Amniotic membrane transplantation for persistent corneal epithelial defects in eyes after penetrating keratoplasty

Seitz

Das

Sauer

et al. 2008

Eye

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Section: Discussionmentioning

confidence: 61%

Amniotic membrane transplantation for persistent corneal epithelial defects in eyes after penetrating keratoplasty

Seitz

Das

Sauer

et al. 2008

Eye

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“…When sutures are used to secure AM for treating human 12 or rabbit 11 corneal epithelial defects, the epithelium might grow under AM, presumably because sutures only provide a focal pin-point seal. In contrast, fibrin glue creates a 2-dimensional seal between AM and the ocular surface, hence not allowing epithelial growth under the membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Recent findings suggest that epithelialization may occur both over and under AM when sutures are used to secure the membrane to rabbit 11 or human 12 corneas. Therefore, one may wonder whether epithelialization may be influenced by transplanted AM if sutures are used.…”

mentioning

confidence: 99%

Sutureless Amniotic Membrane Transplantation for Partial Limbal Stem Cell Deficiency

Kheirkhah

Casas

Raju

et al. 2008

American Journal of Ophthalmology

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PURPOSE-To evaluate the results of sutureless amniotic membrane (AM) transplantation using fibrin glue for reconstructing corneal surfaces with partial limbal stem cell deficiency (LSCD). DESIGN-Retrospective noncomparative interventional case series.METHODS-Eleven eyes of nine patients that had LSCD with 120 degrees to almost 360 degrees of limbal involvement underwent superficial keratectomy to remove the conjunctivalized pannus followed by AM transplantation using fibrin glue. Additional sutureless AM patch (ProKera; BioTissue, Inc, Miami, Florida, USA) was used in seven patients, and mitomycin C was applied on the cornea in four eyes and during fornix reconstruction in seven eyes. The surgery was repeated in three eyes for residual pannus.RESULTS-During a mean follow-up of 14.2 ± 7.7 months (range, six to 26 months), all eyes maintained a smooth and stable corneal epithelial surface without recurrent erosion or persistent epithelial defect, and showed less stromal cloudiness and vascularization. Best-corrected visual acuity improved in nine eyes (81.8%). Corneal epithelialization proceeded by epithelial growth over AM (n = 4), accompanied by dissolution of AM (n = 4) or a combination of both (n = 3). No complication was noted regarding initial or repeated uses of fibrin glue.CONCLUSION-AM transplantation using fibrin glue appears to be a safe and effective method of restoring a stable corneal epithelium for cases with partial LSCD. This approach avoids the need of transplanting limbal epithelial stem cells. THE MAINTENANCE OF A HEALTHY CORNEAL EPITHElium under both normal and traumatic conditions is provided by a unique subpopulation of stem cells located at the limbus. 1,2 When limbal epithelial stem cells are destroyed and/or their supporting stromal environment becomes dysfunctional, a pathological state known as limbal stem cell deficiency (LSCD) develops (for review; see Reference 3 ). LSCD carries the cytologic evidence of conjunctivalization of the corneal surface by a goblet cell-containing conjunctival epithelium. 4 Pathologically, limbal-deficient corneas exhibit destruction of the basement membrane, superficial neovascularization, chronic inflammation, scarring, and poor epithelial integrity (for reviews; see References 3 and 5 ). Clinically, LSCD is a major cause of corneal blindness unable to be corrected by traditional corneal transplantation alone.For eyes inflicted with partial LSCD, where a part of the limbus is damaged, the corneal surface can be reconstructed by debridement of conjunctivalized epithelium with 6-8 or without 9 transplantation of amniotic membrane (AM). Several studies have shown that in eyes with partial LSCD, AM promotes expansion of remaining limbal epithelial stem cells. [6][7][8]10 NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript these studies, sutures were used to secure AM to the cornea following the removal of conjunctivalized pannus from the corneal surface. Recent findings suggest that epithelialization may occur both over and unde...

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“…Aller dings haben mittlerweile einige Studien ergeben, dass die Rezidivrate bei der Am nionmembrantransplantation höher ist [1,7,8,13,20]. Die Amnionmembran stellt die innerste Schicht des Amnionsackes dar und besteht aus avaskulärem Stroma, das von einer dünnen Epithelschicht be deckt ist [11,15]. Kryokonservierte Am nionmembranen stellen eine funktionel le Basalmembran zur Verfügung und er leichtern dadurch die Epithelmigration und adhäsion, während sie gleichzeitig die epitheliale Differenzierung begünsti gen und Apoptose verhindern [18,21].…”

Section: Abb 1 8 Klinische Bilder Von Patient 3 Er Wurde Mit Dem Zwunclassified

Behandlung des schweren rezidivierenden Pterygiums mit Symblepharonbildung

et al. 2013

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Integration Patterns of Cryopreserved Amniotic Membranes into the Human Cornea

Cited by 57 publications

References 31 publications

Amniotic membrane transplantation for persistent corneal epithelial defects in eyes after penetrating keratoplasty

Amniotic membrane transplantation for persistent corneal epithelial defects in eyes after penetrating keratoplasty

Sutureless Amniotic Membrane Transplantation for Partial Limbal Stem Cell Deficiency

Behandlung des schweren rezidivierenden Pterygiums mit Symblepharonbildung

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