Integration of stable carbon isotope, microbial community, dissolved hydrogen gas, and 2HH2O tracer data to assess bioaugmentation for chlorinated ethene degradation in fractured rocks

Bioremediation in Fractured Rock: 2. Mobilization of Chloroethene Compounds from the Rock Matrix

“…In general, similar conditions noted by Révész et al (2014) after 2 years of monitoring continue for the subsequent monitoring through July 2013 ( Figure 3, see Imbrigiotta et al 2017). Concentrations of TCE in 36BR-A and 73BR-D2 continued to be lower in comparison to prebioremediation levels, while concentrations of DCE, VC, and ETH in these monitoring intervals continued to be higher than prebioremediation levels.…”

“…All injected solutions were maintained under anaerobic conditions at land surface prior to their injection. Révész et al (2014) also noted that changes in the CE concentrations observed at 71BR-B and 71BR-C were more difficult to attribute to enhanced reductive dechlorination; CE concentrations for 71BR-C are shown in Figure 3 and the result for 71BR-B are given by Imbrigiotta et al (2017). Révész et al (2014) presented results from the bioremediation for a period of 2 years following the amendment injection.…”

“…In addition, an emulsified vegetable oil solution (EOS™) (Borden et al 2007) that included sodium lactate was injected to act as a fermentable organic substrate to promote the production of H 2 . Additional details of the preparation of the injection solutions and the injection method are given by Révész et al (2014), who provide preliminary estimates of the volume of the amendments injected. Additional details of the preparation of the injection solutions and the injection method are given by Révész et al (2014), who provide preliminary estimates of the volume of the amendments injected.…”

“…Additional details of the preparation of the injection solutions and the injection method are given by Révész et al (2014), who provide preliminary estimates of the volume of the amendments injected. Révész et al (2014) presented results from the bioremediation for a period of 2 years following the amendment injection. An additional 396 L of the emulsified vegetable oil solution was then injected and 177 L of untreated groundwater was then used to flush the injection amendments from the borehole into the formation.…”

Ecophysiology and environmental distribution of organohalide-respiring bacteria

“…This knowledge is crucial to guide design and optimization of ERD efforts but remains limited. To date, ERD related microbial monitoring efforts have mainly focused on quantitative PCR (qPCR) based tracking of key OHRB and their rdhA genes [197,[379][380][381][382] or occasionally of selected non-dechlorinating microbial guilds [361].…”